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- W4300089340 abstract "Dogs frequently suffer from traumatic spinal cord injury (SCI). Most cases of SCI have a favorable prognosis but 40–50% of dogs with paraplegia and absence of nociception do not regain ambulatory abilities, eventually leading to euthanasia. Microglia and infiltrating macrophages play a crucial role in inflammatory process after SCI. However, little is known about microglia/macrophage phenotypes representing a potential target for future therapeutic strategies. In the present study, the microglia/macrophage phenotype was characterized by immunohistochemistry in the morphologically unaltered canine spinal cord (10 control dogs) and during acute and subacute SCI (1–4 and 5–10 days post injury, 9 and 8 dogs, respectively) using antibodies directed against IBA1, MAC387, MHC-II, lysozyme, EGR2, myeloperoxidase, CD18, CD204 and lectin from Griffonia simplicifolia (BS-1). The expression of these markers was also analyzed in the spleen as reference for the phenotype of histiocytic cells. Histological lesions were absent in controls. In acute SCI, 4 dogs showed mild to moderate hemorrhages, 2 dogs bilateral gray matter necrosis and 6 dogs mild multifocal axonal swellings and myelin sheath dilation. One dog with acute SCI did not show histological alterations except for few dilated myelin sheaths. In subacute SCI, variable numbers of gitter cells, axonal changes and dilated myelin sheaths were present in all dogs and large areas of tissue necrosis in 2 dogs. Neuronal chromatolysis was found in 3 dogs with acute and subacute SCI, respectively. In control dogs, microglia/macrophage constitutively expressed IBA1 and rarely other markers. In acute SCI, a similar marker expression was found except for an increase in MAC387-positive cells in the spinal cord white matter due to an infiltration of few blood-borne macrophages. In subacute SCI, increased numbers of microglia/macrophages expressed CD18, CD204 and MHC-II in the gray matter SCI indicating enhanced antigen recognition, processing and presentation as well as cell migration and phagocytosis during this stage. Interestingly, only CD204-positive cells were upregulated in the white matter, which might be related to gray-white matter heterogeneity of microglia as previously described in humans. The present findings contribute to the understanding of the immunological processes during SCI in a large animal model for human SCI." @default.
- W4300089340 created "2022-10-03" @default.
- W4300089340 creator A5015115206 @default.
- W4300089340 creator A5019483995 @default.
- W4300089340 creator A5031612388 @default.
- W4300089340 creator A5039349322 @default.
- W4300089340 date "2022-10-03" @default.
- W4300089340 modified "2023-10-02" @default.
- W4300089340 title "Characterization of microglia/macrophage phenotypes in the spinal cord following intervertebral disc herniation" @default.
- W4300089340 cites W1534280844 @default.
- W4300089340 cites W1540820582 @default.
- W4300089340 cites W1593213689 @default.
- W4300089340 cites W1598872310 @default.
- W4300089340 cites W1963729509 @default.
- W4300089340 cites W1969548331 @default.
- W4300089340 cites W1970495423 @default.
- W4300089340 cites W1970748867 @default.
- W4300089340 cites W1974626393 @default.
- W4300089340 cites W1975011653 @default.
- W4300089340 cites W1978405668 @default.
- W4300089340 cites W1979413998 @default.
- W4300089340 cites W1983285229 @default.
- W4300089340 cites W1985567445 @default.
- W4300089340 cites W1986830129 @default.
- W4300089340 cites W1987286176 @default.
- W4300089340 cites W1988624151 @default.
- W4300089340 cites W1988868629 @default.
- W4300089340 cites W1989556210 @default.
- W4300089340 cites W1989689056 @default.
- W4300089340 cites W1991805788 @default.
- W4300089340 cites W1995365045 @default.
- W4300089340 cites W1997795701 @default.
- W4300089340 cites W1999881215 @default.
- W4300089340 cites W2006517495 @default.
- W4300089340 cites W2010459185 @default.
- W4300089340 cites W2011713316 @default.
- W4300089340 cites W2017913835 @default.
- W4300089340 cites W2018546126 @default.
- W4300089340 cites W2020829506 @default.
- W4300089340 cites W2021342630 @default.
- W4300089340 cites W2023420622 @default.
- W4300089340 cites W2025797605 @default.
- W4300089340 cites W2025967875 @default.
- W4300089340 cites W2026359714 @default.
- W4300089340 cites W2026469557 @default.
- W4300089340 cites W2027673638 @default.
- W4300089340 cites W2034199603 @default.
- W4300089340 cites W2035779766 @default.
- W4300089340 cites W2037303707 @default.
- W4300089340 cites W2038991568 @default.
- W4300089340 cites W2039334993 @default.
- W4300089340 cites W2039420382 @default.
- W4300089340 cites W2044373854 @default.
- W4300089340 cites W2049585466 @default.
- W4300089340 cites W2053176712 @default.
- W4300089340 cites W2053684855 @default.
- W4300089340 cites W2054640342 @default.
- W4300089340 cites W2055267476 @default.
- W4300089340 cites W2059028717 @default.
- W4300089340 cites W2060197017 @default.
- W4300089340 cites W2060896724 @default.
- W4300089340 cites W2063339343 @default.
- W4300089340 cites W2068467835 @default.
- W4300089340 cites W2074010869 @default.
- W4300089340 cites W2080008292 @default.
- W4300089340 cites W2082184929 @default.
- W4300089340 cites W2085716611 @default.
- W4300089340 cites W2085868504 @default.
- W4300089340 cites W2096129702 @default.
- W4300089340 cites W2096182847 @default.
- W4300089340 cites W2107446553 @default.
- W4300089340 cites W2109759840 @default.
- W4300089340 cites W2112516157 @default.
- W4300089340 cites W2116077352 @default.
- W4300089340 cites W2116605894 @default.
- W4300089340 cites W2118851633 @default.
- W4300089340 cites W2124342825 @default.
- W4300089340 cites W2127522048 @default.
- W4300089340 cites W2129861885 @default.
- W4300089340 cites W2130408786 @default.
- W4300089340 cites W2131832510 @default.
- W4300089340 cites W2140967407 @default.
- W4300089340 cites W2142958905 @default.
- W4300089340 cites W2144623814 @default.
- W4300089340 cites W2146358032 @default.
- W4300089340 cites W2152362568 @default.
- W4300089340 cites W2160838897 @default.
- W4300089340 cites W2161456562 @default.
- W4300089340 cites W2280077905 @default.
- W4300089340 cites W2314358957 @default.
- W4300089340 cites W2407337075 @default.
- W4300089340 cites W2409214245 @default.
- W4300089340 cites W2462418565 @default.
- W4300089340 cites W2509443154 @default.
- W4300089340 cites W2591533639 @default.
- W4300089340 cites W2602024874 @default.
- W4300089340 cites W27022565 @default.
- W4300089340 cites W2768859792 @default.
- W4300089340 cites W2804025158 @default.
- W4300089340 cites W2921234203 @default.