Matches in SemOpenAlex for { <https://semopenalex.org/work/W4300152324> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W4300152324 abstract "Chromosomal aneuploidy, one of the main causes leading to embryonic development arrest, implantation failure, or pregnancy loss, has been well documented in human embryos. Preimplantation genetic testing for aneuploidy (PGT-A) is a genetic test that significantly improves reproductive outcomes by detecting chromosomal abnormalities of embryos. Next-generation sequencing (NGS) provides a high-throughput and cost-effective approach for genetic analysis and has shown clinical applicability in PGT-A. Here, we present a rapid and low-cost semiconductor sequencing-based NGS method for screening of aneuploidy in embryos. The first step of the workflow is whole genome amplification (WGA) of the biopsied embryo specimen, followed by construction of sequencing library, and subsequent sequencing on the semiconductor sequencing system. Generally, for a PGT-A application, 24 samples can be loaded and sequenced on each chip generating 60−80 million reads at an average read length of 150 base pairs. The method provides a refined protocol for performing template amplification and enrichment of sequencing library, making the PGT-A detection reproducible, high-throughput, cost-efficient, and timesaving. The running time of this semiconductor sequencer is only 2−4 hours, shortening the turnaround time from receiving samples to issuing reports into 5 days. All these advantages make this assay an ideal method to detect chromosomal aneuploidies from embryos and thus, facilitate its wide application in PGT-A." @default.
- W4300152324 created "2022-10-03" @default.
- W4300152324 creator A5005131762 @default.
- W4300152324 creator A5014272356 @default.
- W4300152324 creator A5015186381 @default.
- W4300152324 creator A5019291477 @default.
- W4300152324 creator A5020124067 @default.
- W4300152324 creator A5020872625 @default.
- W4300152324 creator A5029498306 @default.
- W4300152324 creator A5050264113 @default.
- W4300152324 creator A5073082306 @default.
- W4300152324 creator A5081884704 @default.
- W4300152324 date "2019-08-25" @default.
- W4300152324 modified "2023-09-26" @default.
- W4300152324 title "Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy" @default.
- W4300152324 doi "https://doi.org/10.3791/59273-v" @default.
- W4300152324 hasPublicationYear "2019" @default.
- W4300152324 type Work @default.
- W4300152324 citedByCount "0" @default.
- W4300152324 crossrefType "journal-article" @default.
- W4300152324 hasAuthorship W4300152324A5005131762 @default.
- W4300152324 hasAuthorship W4300152324A5014272356 @default.
- W4300152324 hasAuthorship W4300152324A5015186381 @default.
- W4300152324 hasAuthorship W4300152324A5019291477 @default.
- W4300152324 hasAuthorship W4300152324A5020124067 @default.
- W4300152324 hasAuthorship W4300152324A5020872625 @default.
- W4300152324 hasAuthorship W4300152324A5029498306 @default.
- W4300152324 hasAuthorship W4300152324A5050264113 @default.
- W4300152324 hasAuthorship W4300152324A5073082306 @default.
- W4300152324 hasAuthorship W4300152324A5081884704 @default.
- W4300152324 hasConcept C104317684 @default.
- W4300152324 hasConcept C192800701 @default.
- W4300152324 hasConcept C196843134 @default.
- W4300152324 hasConcept C2778805741 @default.
- W4300152324 hasConcept C2779672484 @default.
- W4300152324 hasConcept C2780673598 @default.
- W4300152324 hasConcept C30481170 @default.
- W4300152324 hasConcept C51679486 @default.
- W4300152324 hasConcept C54355233 @default.
- W4300152324 hasConcept C70721500 @default.
- W4300152324 hasConcept C86803240 @default.
- W4300152324 hasConceptScore W4300152324C104317684 @default.
- W4300152324 hasConceptScore W4300152324C192800701 @default.
- W4300152324 hasConceptScore W4300152324C196843134 @default.
- W4300152324 hasConceptScore W4300152324C2778805741 @default.
- W4300152324 hasConceptScore W4300152324C2779672484 @default.
- W4300152324 hasConceptScore W4300152324C2780673598 @default.
- W4300152324 hasConceptScore W4300152324C30481170 @default.
- W4300152324 hasConceptScore W4300152324C51679486 @default.
- W4300152324 hasConceptScore W4300152324C54355233 @default.
- W4300152324 hasConceptScore W4300152324C70721500 @default.
- W4300152324 hasConceptScore W4300152324C86803240 @default.
- W4300152324 hasIssue "150" @default.
- W4300152324 hasLocation W43001523241 @default.
- W4300152324 hasOpenAccess W4300152324 @default.
- W4300152324 hasPrimaryLocation W43001523241 @default.
- W4300152324 hasRelatedWork W1968021427 @default.
- W4300152324 hasRelatedWork W2157560711 @default.
- W4300152324 hasRelatedWork W2805233078 @default.
- W4300152324 hasRelatedWork W2969554645 @default.
- W4300152324 hasRelatedWork W3137268669 @default.
- W4300152324 hasRelatedWork W3145667584 @default.
- W4300152324 hasRelatedWork W4205741757 @default.
- W4300152324 hasRelatedWork W4206394424 @default.
- W4300152324 hasRelatedWork W4300152324 @default.
- W4300152324 hasRelatedWork W4319601417 @default.
- W4300152324 isParatext "false" @default.
- W4300152324 isRetracted "false" @default.
- W4300152324 workType "article" @default.