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- W4300432848 abstract "This chapter discusses the methods for injecting messenger RNA (mRNA) to test directly whether mRNAs limit the rate of protein synthesis in sea urchin eggs, or whether, as in Xenopus, some other component of the translational machinery is rate limiting. Since sea urchin eggs are much smaller than Xenopus oocytes (80 to 180 pm in diameter as compared to 1.2 mm), a large number of eggs are required to synthesize sufficient peptides for an accurate statistical analysis. Thus, it is necessary to devise methods for injecting large numbers of eggs in a relatively short time. There are methods designed for the rapid injection of large numbers of echinoid eggs or zygotes, they should also be useful for injecting a variety of cell types or for fewer cells. The methods discuss to inject large numbers of echinoid eggs and zygotes with globin mRNA and label them with tritiated amino acids. The products can be quantitatively analyzed to determine the amounts of peptide synthesis. The injections can be performed quickly and require relatively simple and inexpensive equipment." @default.
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- W4300432848 date "1986-01-01" @default.
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- W4300432848 title "Chapter 22 Rapid Repetitive Microinjection" @default.
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- W4300432848 doi "https://doi.org/10.1016/s0091-679x(08)60360-x" @default.
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