FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4300476064> ?p ?o ?g. }

• W4300476064 endingPage "4816" @default.
• W4300476064 startingPage "4806" @default.
• W4300476064 abstract "Research Article16 September 1996free access Structure-function analysis of the Escherichia coli GrpE heat shock protein. B. Wu B. Wu Department of Oncological Sciences, University of Utah School of Medicine, Salt Lake City 84132, USA. Search for more papers by this author A. Wawrzynow A. Wawrzynow Department of Oncological Sciences, University of Utah School of Medicine, Salt Lake City 84132, USA. Search for more papers by this author M. Zylicz M. Zylicz Department of Oncological Sciences, University of Utah School of Medicine, Salt Lake City 84132, USA. Search for more papers by this author C. Georgopoulos C. Georgopoulos Department of Oncological Sciences, University of Utah School of Medicine, Salt Lake City 84132, USA. Search for more papers by this author B. Wu B. Wu Department of Oncological Sciences, University of Utah School of Medicine, Salt Lake City 84132, USA. Search for more papers by this author A. Wawrzynow A. Wawrzynow Department of Oncological Sciences, University of Utah School of Medicine, Salt Lake City 84132, USA. Search for more papers by this author M. Zylicz M. Zylicz Department of Oncological Sciences, University of Utah School of Medicine, Salt Lake City 84132, USA. Search for more papers by this author C. Georgopoulos C. Georgopoulos Department of Oncological Sciences, University of Utah School of Medicine, Salt Lake City 84132, USA. Search for more papers by this author Author Information B. Wu1, A. Wawrzynow1, M. Zylicz1 and C. Georgopoulos1 1Department of Oncological Sciences, University of Utah School of Medicine, Salt Lake City 84132, USA. The EMBO Journal (1996)15:4806-4816https://doi.org/10.1002/j.1460-2075.1996.tb00861.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info We have isolated various missense mutations in the essential grpE gene of Escherichia coli based on the inability to propagate bacteriophage lambda. To better understand the biochemical mechanisms of GrpE action in various biological processes, six mutant proteins were overexpressed and purified. All of them, GrpE103, GrpE66, GrpE2/280, GrpE17, GrpE13a and GrpE25, have single amino acid substitutions located in highly conserved regions throughout the GrpE sequence. The biochemical defects of each mutant GrpE protein were identified by examining their abilities to: (i) support in vitro lambda DNA replication; (ii) stimulate the weak ATPase activity of DnaK; (iii) dimerize and oligomerize, as judged by glutaraldehyde crosslinking and HPLC size chromatography; (iv) interact with wild-type DnaK protein using either an ELISA assay, glutaraldehyde crosslinking or HPLC size chromatography. Our results suggest that GrpE can exist in a dimeric or oligomeric form, depending on its relative concentration, and that it dimerizes/oligomerizes through its N-terminal region, most likely through a computer predicted coiled-coil region. Analysis of several mutant GrpE proteins indicates that an oligomer of GrpE is the most active form that interacts stably with DnaK and that the interaction is vital for GrpE biological function. Our results also demonstrate that both the N-terminal and C-terminal regions are important for GrpE function in lambda DNA replication and its co-chaperone activity with DnaK. Previous ArticleNext Article Volume 15Issue 181 September 1996In this issue RelatedDetailsLoading ..." @default.
• W4300476064 created "2022-10-03" @default.
• W4300476064 creator A5015198268 @default.
• W4300476064 creator A5057641433 @default.
• W4300476064 creator A5073515303 @default.
• W4300476064 creator A5077581897 @default.
• W4300476064 date "1996-09-01" @default.
• W4300476064 modified "2023-09-30" @default.
• W4300476064 title "Structure-function analysis of the Escherichia coli GrpE heat shock protein." @default.
• W4300476064 doi "https://doi.org/10.1002/j.1460-2075.1996.tb00861.x" @default.
• W4300476064 hasPublicationYear "1996" @default.
• W4300476064 type Work @default.
• W4300476064 citedByCount "36" @default.
• W4300476064 countsByYear W43004760642012 @default.
• W4300476064 countsByYear W43004760642013 @default.
• W4300476064 countsByYear W43004760642019 @default.
• W4300476064 countsByYear W43004760642020 @default.
• W4300476064 countsByYear W43004760642023 @default.
• W4300476064 crossrefType "journal-article" @default.
• W4300476064 hasAuthorship W4300476064A5015198268 @default.
• W4300476064 hasAuthorship W4300476064A5057641433 @default.
• W4300476064 hasAuthorship W4300476064A5073515303 @default.
• W4300476064 hasAuthorship W4300476064A5077581897 @default.
• W4300476064 hasBestOaLocation W43004760642 @default.
• W4300476064 hasConcept C109007969 @default.
• W4300476064 hasConcept C151730666 @default.
• W4300476064 hasConcept C161191863 @default.
• W4300476064 hasConcept C2991991741 @default.
• W4300476064 hasConcept C41008148 @default.
• W4300476064 hasConcept C86803240 @default.
• W4300476064 hasConceptScore W4300476064C109007969 @default.
• W4300476064 hasConceptScore W4300476064C151730666 @default.
• W4300476064 hasConceptScore W4300476064C161191863 @default.
• W4300476064 hasConceptScore W4300476064C2991991741 @default.
• W4300476064 hasConceptScore W4300476064C41008148 @default.
• W4300476064 hasConceptScore W4300476064C86803240 @default.
• W4300476064 hasIssue "18" @default.
• W4300476064 hasLocation W43004760641 @default.
• W4300476064 hasLocation W43004760642 @default.
• W4300476064 hasOpenAccess W4300476064 @default.
• W4300476064 hasPrimaryLocation W43004760641 @default.
• W4300476064 hasRelatedWork W1567067073 @default.
• W4300476064 hasRelatedWork W2318398610 @default.
• W4300476064 hasRelatedWork W2335182518 @default.
• W4300476064 hasRelatedWork W2797651085 @default.
• W4300476064 hasRelatedWork W2911578961 @default.
• W4300476064 hasRelatedWork W2989063522 @default.
• W4300476064 hasRelatedWork W341798316 @default.
• W4300476064 hasRelatedWork W4232624405 @default.
• W4300476064 hasRelatedWork W4237086420 @default.
• W4300476064 hasRelatedWork W4252761805 @default.
• W4300476064 hasVolume "15" @default.
• W4300476064 isParatext "false" @default.
• W4300476064 isRetracted "false" @default.
• W4300476064 workType "article" @default.