Matches in SemOpenAlex for { <https://semopenalex.org/work/W4300594511> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W4300594511 endingPage "217" @default.
- W4300594511 startingPage "211" @default.
- W4300594511 abstract "BACKGROUND: Nowadays there are a lot of data to suggest about an important role of ghrelin signaling system in stress response and reinforcement mechanisms. There is a common opinion that a protein kinase system plays a role of a second intracellular messenger for ghrelin system.
 AIM: So, the aim of our study was to measure the total protein kinase activity in brain structures of Danio rerio. The all procedures have been approved according to the Local Ethical Committee of Institute of Experimental Medicine.
 MATERIALS AND METHODS: In our study it has been used 50 specimen Danio rerio 68 months of age. There also has been used a predator (Hypsophrys nicaraguensis). During experiment a fish has been firstly placed into a tank of water with a dissolved pharmacological substance for 5 min, then has been transferred into a tank with predator also for 5 min. Two ghrelin antagonists have been used in our experiment in 0.333 mg/l dosage [D-Lys3]-GHRP-6 and Agrelax, a recombinant peptide analogue of ghrelin with molecular weight 3.5 kD, which has been invented in our Institute. The brain has been divided into three anatomical parts: telencephalon, midbrain and cerebellum. After that the material for kinase activity assay was made (ADP sensor Universal Kinase Activity Assay kit, BioVision).We also have used GraphPad Prism 6 for statistical estimation. KolmogorovSmirnov test MannWhitney U-test have been used as statistical tests.
 RESULTS: In control group, the kinase activity has been determined in all tested brain structures. After predator exposure, the kinase activity has significantly changed. After [D-Lys3]-GHRP-6 administration there has not been determined any protein kinase activity in all brain structures. In the cerebellum the kinase activity has been the highest after Agrelax administration. Protein kinase system demonstrates significant stress response.
 CONCLUSIONS. Pharmacological action on ghrelin receptors leads to changes in kinase system in Danio rerios brain structures." @default.
- W4300594511 created "2022-10-03" @default.
- W4300594511 creator A5006276779 @default.
- W4300594511 creator A5015686594 @default.
- W4300594511 creator A5017213750 @default.
- W4300594511 creator A5021582403 @default.
- W4300594511 creator A5045343935 @default.
- W4300594511 creator A5059244884 @default.
- W4300594511 date "2022-10-03" @default.
- W4300594511 modified "2023-09-26" @default.
- W4300594511 title "The changes of protein kinases activities in the brain structures after ghrelin antagonists administration in previously stressed <i>Danio rerio</i>" @default.
- W4300594511 cites W1981250920 @default.
- W4300594511 cites W1983769105 @default.
- W4300594511 cites W1993971610 @default.
- W4300594511 cites W1994554353 @default.
- W4300594511 cites W2027963861 @default.
- W4300594511 cites W2046084873 @default.
- W4300594511 cites W2067273260 @default.
- W4300594511 cites W2109458697 @default.
- W4300594511 cites W2118650779 @default.
- W4300594511 cites W2155910632 @default.
- W4300594511 cites W3020691816 @default.
- W4300594511 doi "https://doi.org/10.17816/rcf202211-217" @default.
- W4300594511 hasPublicationYear "2022" @default.
- W4300594511 type Work @default.
- W4300594511 citedByCount "0" @default.
- W4300594511 crossrefType "journal-article" @default.
- W4300594511 hasAuthorship W4300594511A5006276779 @default.
- W4300594511 hasAuthorship W4300594511A5015686594 @default.
- W4300594511 hasAuthorship W4300594511A5017213750 @default.
- W4300594511 hasAuthorship W4300594511A5021582403 @default.
- W4300594511 hasAuthorship W4300594511A5045343935 @default.
- W4300594511 hasAuthorship W4300594511A5059244884 @default.
- W4300594511 hasBestOaLocation W43005945111 @default.
- W4300594511 hasConcept C104317684 @default.
- W4300594511 hasConcept C126322002 @default.
- W4300594511 hasConcept C134018914 @default.
- W4300594511 hasConcept C170493617 @default.
- W4300594511 hasConcept C184235292 @default.
- W4300594511 hasConcept C185592680 @default.
- W4300594511 hasConcept C188382862 @default.
- W4300594511 hasConcept C18903297 @default.
- W4300594511 hasConcept C2776878037 @default.
- W4300594511 hasConcept C2779357093 @default.
- W4300594511 hasConcept C2780384893 @default.
- W4300594511 hasConcept C529278444 @default.
- W4300594511 hasConcept C533869091 @default.
- W4300594511 hasConcept C55493867 @default.
- W4300594511 hasConcept C71924100 @default.
- W4300594511 hasConcept C86803240 @default.
- W4300594511 hasConcept C95444343 @default.
- W4300594511 hasConcept C96857902 @default.
- W4300594511 hasConcept C97029542 @default.
- W4300594511 hasConcept C98274493 @default.
- W4300594511 hasConceptScore W4300594511C104317684 @default.
- W4300594511 hasConceptScore W4300594511C126322002 @default.
- W4300594511 hasConceptScore W4300594511C134018914 @default.
- W4300594511 hasConceptScore W4300594511C170493617 @default.
- W4300594511 hasConceptScore W4300594511C184235292 @default.
- W4300594511 hasConceptScore W4300594511C185592680 @default.
- W4300594511 hasConceptScore W4300594511C188382862 @default.
- W4300594511 hasConceptScore W4300594511C18903297 @default.
- W4300594511 hasConceptScore W4300594511C2776878037 @default.
- W4300594511 hasConceptScore W4300594511C2779357093 @default.
- W4300594511 hasConceptScore W4300594511C2780384893 @default.
- W4300594511 hasConceptScore W4300594511C529278444 @default.
- W4300594511 hasConceptScore W4300594511C533869091 @default.
- W4300594511 hasConceptScore W4300594511C55493867 @default.
- W4300594511 hasConceptScore W4300594511C71924100 @default.
- W4300594511 hasConceptScore W4300594511C86803240 @default.
- W4300594511 hasConceptScore W4300594511C95444343 @default.
- W4300594511 hasConceptScore W4300594511C96857902 @default.
- W4300594511 hasConceptScore W4300594511C97029542 @default.
- W4300594511 hasConceptScore W4300594511C98274493 @default.
- W4300594511 hasIssue "2" @default.
- W4300594511 hasLocation W43005945111 @default.
- W4300594511 hasOpenAccess W4300594511 @default.
- W4300594511 hasPrimaryLocation W43005945111 @default.
- W4300594511 hasRelatedWork W2003577251 @default.
- W4300594511 hasRelatedWork W2025062373 @default.
- W4300594511 hasRelatedWork W2029765733 @default.
- W4300594511 hasRelatedWork W2048194663 @default.
- W4300594511 hasRelatedWork W2093337002 @default.
- W4300594511 hasRelatedWork W2112085129 @default.
- W4300594511 hasRelatedWork W2126643606 @default.
- W4300594511 hasRelatedWork W2127596209 @default.
- W4300594511 hasRelatedWork W2140569197 @default.
- W4300594511 hasRelatedWork W2160522268 @default.
- W4300594511 hasVolume "20" @default.
- W4300594511 isParatext "false" @default.
- W4300594511 isRetracted "false" @default.
- W4300594511 workType "article" @default.