FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4300688783> ?p ?o ?g. }

• W4300688783 abstract "Two-component systems (TCSs) act as common regulatory systems allowing bacteria to detect and respond to multiple environmental stimuli, including cell envelope stress. The MtrAB TCS of Actinobacteria is critical for cell wall homeostasis, cell proliferation, osmoprotection, and antibiotic resistance, and thus is found to be highly conserved across this phylum. However, how precisely the MtrAB TCS regulates cellular homeostasis in response to environmental stress remains unclear. Here, we show that the MtrAB TCS plays an important role in the tolerance to different types of cell envelope stresses, including environmental stresses (i.e., oxidative stress, lysozyme, SDS, osmotic pressure, and alkaline pH stresses) and envelope-targeting antibiotics (i.e., isoniazid, ethambutol, glycopeptide, and β-lactam antibiotics) in Dietzia sp. DQ12-45-1b. An mtrAB mutant strain exhibited slower growth compared to the wild-type strain and was characterized by abnormal cell shapes when exposed to various environmental stresses. Moreover, deletion of mtrAB resulted in decreased resistance to isoniazid, ethambutol, and β-lactam antibiotics. Further, Cleavage under targets and tagmentation sequencing (CUT&Tag-seq) and electrophoretic mobility shift assays (EMSAs) revealed that MtrA binds the promoters of genes involved in peptidoglycan biosynthesis (ldtB, ldtA, murJ), hydrolysis (GJR88_03483, GJR88_4713), and cell division (ftsE). Together, our findings demonstrated that the MtrAB TCS is essential for the survival of Dietzia sp. DQ12-45-1b under various cell envelope stresses, primarily by controlling multiple downstream cellular pathways. Our work suggests that TCSs act as global sensors and regulators in maintaining cellular homeostasis, such as during episodes of various environmental stresses. The present study should shed light on the understanding of mechanisms for bacterial adaptivity to extreme environments. IMPORTANCE The multilayered cell envelope is the first line of bacterial defense against various extreme environments. Bacteria utilize a large number of sensing and regulatory systems to maintain cell envelope homeostasis under multiple stress conditions. The two-component system (TCS) is the main sensing and responding apparatus for environmental adaptation. The MtrAB TCS highly conserved in Actinobacteria is critical for cell wall homeostasis, cell proliferation, osmoprotection, and antibiotic resistance. However, how MtrAB works with regard to signals impacting changes to the cell envelope is not fully understood. Here, we found that in the Actinobacterium Dietzia sp. DQ12-45-1b, a TCS named MtrAB is pivotal for ensuring normal cell growth as well as maintaining proper cell morphology in response to various cell envelope stresses, namely, by regulating the expression of cell envelope-related genes. Our findings should greatly advance our understanding of the adaptive mechanisms responsible for maintaining cell integrity in times of sustained environmental shocks." @default.
• W4300688783 created "2022-10-04" @default.
• W4300688783 creator A5000701455 @default.
• W4300688783 creator A5071925119 @default.
• W4300688783 creator A5078415347 @default.
• W4300688783 creator A5084642040 @default.
• W4300688783 date "2022-10-26" @default.
• W4300688783 modified "2023-10-18" @default.
• W4300688783 title "The Roles of the Two-Component System, MtrAB, in Response to Diverse Cell Envelope Stresses in <i>Dietzia</i> sp. DQ12-45-1b" @default.
• W4300688783 cites W122179485 @default.
• W4300688783 cites W1533538206 @default.
• W4300688783 cites W1546022138 @default.
• W4300688783 cites W1884131007 @default.
• W4300688783 cites W1966023562 @default.
• W4300688783 cites W1973974403 @default.
• W4300688783 cites W1974155854 @default.
• W4300688783 cites W1977724702 @default.
• W4300688783 cites W1977836482 @default.
• W4300688783 cites W1983672257 @default.
• W4300688783 cites W1990890478 @default.
• W4300688783 cites W1992243689 @default.
• W4300688783 cites W1992489434 @default.
• W4300688783 cites W1997248888 @default.
• W4300688783 cites W2000240522 @default.
• W4300688783 cites W2005548636 @default.
• W4300688783 cites W2005889911 @default.
• W4300688783 cites W2006414302 @default.
• W4300688783 cites W2009933943 @default.
• W4300688783 cites W2011128355 @default.
• W4300688783 cites W2014794500 @default.
• W4300688783 cites W2017567641 @default.
• W4300688783 cites W2019249774 @default.
• W4300688783 cites W2026377947 @default.
• W4300688783 cites W2029207581 @default.
• W4300688783 cites W2030582309 @default.
• W4300688783 cites W2047040799 @default.
• W4300688783 cites W2048614879 @default.
• W4300688783 cites W2053618304 @default.
• W4300688783 cites W2055234236 @default.
• W4300688783 cites W2067572953 @default.
• W4300688783 cites W2070174460 @default.
• W4300688783 cites W2074107240 @default.
• W4300688783 cites W2078328454 @default.
• W4300688783 cites W2091538154 @default.
• W4300688783 cites W2093660582 @default.
• W4300688783 cites W2093712250 @default.
• W4300688783 cites W2095836360 @default.
• W4300688783 cites W2095958235 @default.
• W4300688783 cites W2097403532 @default.
• W4300688783 cites W2097706568 @default.
• W4300688783 cites W2099883798 @default.
• W4300688783 cites W2100122962 @default.
• W4300688783 cites W2102850224 @default.
• W4300688783 cites W2105289883 @default.
• W4300688783 cites W2106882534 @default.
• W4300688783 cites W2107277218 @default.
• W4300688783 cites W2109834591 @default.
• W4300688783 cites W2110065044 @default.
• W4300688783 cites W2110347352 @default.
• W4300688783 cites W2120704894 @default.
• W4300688783 cites W2124851289 @default.
• W4300688783 cites W2130771659 @default.
• W4300688783 cites W2133342418 @default.
• W4300688783 cites W2140785597 @default.
• W4300688783 cites W2145111771 @default.
• W4300688783 cites W2149811207 @default.
• W4300688783 cites W2151060181 @default.
• W4300688783 cites W2157009395 @default.
• W4300688783 cites W2159145578 @default.
• W4300688783 cites W2164990150 @default.
• W4300688783 cites W2192080449 @default.
• W4300688783 cites W2203933710 @default.
• W4300688783 cites W2413829872 @default.
• W4300688783 cites W2568090253 @default.
• W4300688783 cites W2579958964 @default.
• W4300688783 cites W2601963881 @default.
• W4300688783 cites W2752330901 @default.
• W4300688783 cites W2791576242 @default.
• W4300688783 cites W2897723297 @default.
• W4300688783 cites W2900559619 @default.
• W4300688783 cites W2920191405 @default.
• W4300688783 cites W2939226595 @default.
• W4300688783 cites W2947447072 @default.
• W4300688783 cites W2950985970 @default.
• W4300688783 cites W2951361566 @default.
• W4300688783 cites W2969337649 @default.
• W4300688783 cites W2981877912 @default.
• W4300688783 cites W3044407339 @default.
• W4300688783 cites W3092260550 @default.
• W4300688783 cites W3092385290 @default.
• W4300688783 cites W3130450984 @default.
• W4300688783 cites W3176693227 @default.
• W4300688783 doi "https://doi.org/10.1128/aem.01337-22" @default.
• W4300688783 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36190258" @default.
• W4300688783 hasPublicationYear "2022" @default.
• W4300688783 type Work @default.
• W4300688783 citedByCount "1" @default.
• W4300688783 countsByYear W43006887832023 @default.
• W4300688783 crossrefType "journal-article" @default.
• W4300688783 hasAuthorship W4300688783A5000701455 @default.

• next