Matches in SemOpenAlex for { <https://semopenalex.org/work/W4300689614> ?p ?o ?g. }
- W4300689614 endingPage "12989" @default.
- W4300689614 startingPage "12982" @default.
- W4300689614 abstract "Lactobacillus S-layer protein (SLP) is a biologically active protein on the cell surface. To further elucidate the structures and functions of SLP in Lactobacillus acidophilus CICC 6074, this study was conducted to identify the functional domains of SLP which is responsible for cell wall anchoring, self-assembly, and adhesion. The gene (slpA) of L. acidophilus CICC 6074 SLP was amplified by polymerase chain reaction and speculated functional domains. Fusion proteins of C-terminal truncations from SLP were exogenously expressed in Escherichia coli BL21 (DE3). FITC-labeling N-terminal truncations of SLP were synthesized. The C-terminal domain was more likely to be the binding region, and the cell wall-anchored receptor of SLP was teichoic acid. Furthermore, N-terminal truncations could self-assemble to milk fat globule membrane polar lipid liposomes observed using a fluorescence microscope. Notably, SAN1 (region 32-55) of N-terminal truncations was mainly responsible for the adhesion of SLP to HT-29 cells. These results showed that SLP played a crucial role in the functions of L. acidophilus CICC 6074, which might be of significant reference value for future studies." @default.
- W4300689614 created "2022-10-04" @default.
- W4300689614 creator A5015105154 @default.
- W4300689614 creator A5017941291 @default.
- W4300689614 creator A5035701638 @default.
- W4300689614 creator A5048003531 @default.
- W4300689614 creator A5050745133 @default.
- W4300689614 creator A5051777223 @default.
- W4300689614 creator A5060941170 @default.
- W4300689614 creator A5064583200 @default.
- W4300689614 creator A5068741303 @default.
- W4300689614 creator A5083956383 @default.
- W4300689614 creator A5087880805 @default.
- W4300689614 creator A5090547325 @default.
- W4300689614 date "2022-10-03" @default.
- W4300689614 modified "2023-10-17" @default.
- W4300689614 title "Identification and Characterization of Domains Responsible for Cell Wall Binding, Self-Assembly, and Adhesion of S-layer Protein from <i>Lactobacillus acidophilus</i> CICC 6074" @default.
- W4300689614 cites W1481121752 @default.
- W4300689614 cites W1635858559 @default.
- W4300689614 cites W1973364946 @default.
- W4300689614 cites W1980604167 @default.
- W4300689614 cites W1999199949 @default.
- W4300689614 cites W2023623126 @default.
- W4300689614 cites W2025079144 @default.
- W4300689614 cites W2033370704 @default.
- W4300689614 cites W2055554679 @default.
- W4300689614 cites W2059202139 @default.
- W4300689614 cites W2090084901 @default.
- W4300689614 cites W2124805887 @default.
- W4300689614 cites W2128832395 @default.
- W4300689614 cites W2138816428 @default.
- W4300689614 cites W2140210011 @default.
- W4300689614 cites W2155317743 @default.
- W4300689614 cites W2161976205 @default.
- W4300689614 cites W2267241866 @default.
- W4300689614 cites W2481955986 @default.
- W4300689614 cites W2593325389 @default.
- W4300689614 cites W2593944659 @default.
- W4300689614 cites W2602615970 @default.
- W4300689614 cites W2782151363 @default.
- W4300689614 cites W2791901385 @default.
- W4300689614 cites W2805753499 @default.
- W4300689614 cites W2810922516 @default.
- W4300689614 cites W2942734021 @default.
- W4300689614 cites W2964116700 @default.
- W4300689614 cites W2997773857 @default.
- W4300689614 cites W3003587605 @default.
- W4300689614 cites W3033070533 @default.
- W4300689614 cites W3044602743 @default.
- W4300689614 cites W3048996767 @default.
- W4300689614 cites W3092289941 @default.
- W4300689614 cites W3171396921 @default.
- W4300689614 cites W3177344432 @default.
- W4300689614 cites W3203645219 @default.
- W4300689614 cites W4207018949 @default.
- W4300689614 cites W4211072623 @default.
- W4300689614 cites W4213009004 @default.
- W4300689614 doi "https://doi.org/10.1021/acs.jafc.2c03907" @default.
- W4300689614 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36190122" @default.
- W4300689614 hasPublicationYear "2022" @default.
- W4300689614 type Work @default.
- W4300689614 citedByCount "1" @default.
- W4300689614 countsByYear W43006896142023 @default.
- W4300689614 crossrefType "journal-article" @default.
- W4300689614 hasAuthorship W4300689614A5015105154 @default.
- W4300689614 hasAuthorship W4300689614A5017941291 @default.
- W4300689614 hasAuthorship W4300689614A5035701638 @default.
- W4300689614 hasAuthorship W4300689614A5048003531 @default.
- W4300689614 hasAuthorship W4300689614A5050745133 @default.
- W4300689614 hasAuthorship W4300689614A5051777223 @default.
- W4300689614 hasAuthorship W4300689614A5060941170 @default.
- W4300689614 hasAuthorship W4300689614A5064583200 @default.
- W4300689614 hasAuthorship W4300689614A5068741303 @default.
- W4300689614 hasAuthorship W4300689614A5083956383 @default.
- W4300689614 hasAuthorship W4300689614A5087880805 @default.
- W4300689614 hasAuthorship W4300689614A5090547325 @default.
- W4300689614 hasConcept C104317684 @default.
- W4300689614 hasConcept C121332964 @default.
- W4300689614 hasConcept C123894998 @default.
- W4300689614 hasConcept C125235067 @default.
- W4300689614 hasConcept C12554922 @default.
- W4300689614 hasConcept C1491633281 @default.
- W4300689614 hasConcept C169274487 @default.
- W4300689614 hasConcept C170344550 @default.
- W4300689614 hasConcept C178790620 @default.
- W4300689614 hasConcept C185592680 @default.
- W4300689614 hasConcept C2777221637 @default.
- W4300689614 hasConcept C2780255968 @default.
- W4300689614 hasConcept C40767141 @default.
- W4300689614 hasConcept C515207424 @default.
- W4300689614 hasConcept C523546767 @default.
- W4300689614 hasConcept C54355233 @default.
- W4300689614 hasConcept C547475151 @default.
- W4300689614 hasConcept C55493867 @default.
- W4300689614 hasConcept C57711820 @default.
- W4300689614 hasConcept C62520636 @default.
- W4300689614 hasConcept C84416704 @default.
- W4300689614 hasConcept C85789140 @default.