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- W4300692918 abstract "In the early 1950s Christian de Duve was working at Louvain University in Belgium, where he was interested in the biological role of the enzyme, acid phosphatase (figure 6.1). In order to study its subcellular distribution, he was applying the technique of differential centrifugation (section 2.2.1) to isotonic homogenates of rat liver. he found that the activity of acid phosphatase in a freshly prepared homogenate was much less than that in a homogenate that had been left on the bench overnight or that had been frozen and thaewed. These results led de Duve to formulate the concept of structure-linked latency: that inside the cell some enzymes were shielded from their substrate by an impermeable structure of some kind. What was the nature of this structure?" @default.
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- W4300692918 date "1989-01-01" @default.
- W4300692918 modified "2023-09-27" @default.
- W4300692918 title "The Lysosome" @default.
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- W4300692918 doi "https://doi.org/10.1007/978-1-349-19781-1_6" @default.
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