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- W4300792798 abstract "Abstract The extractable protein in bark tissue of 1-year-old shoots of apple trees ( Malus domestica Borkh. cv. Golden Delicious on Mailing (M) 26 rootstocks) declined dramatically during spring growth, while amino acids increased. Extensive degradation of protein was first visible at the silver-tip stage of growth. Prior to this period, some redistribution of nitrogenous compounds was indicated by the increase in amino acids while protein remained unchanged. Total extractable nitrogen declined during spring growth, indicating that it was mobilized to developing tissues. In vitro activity of an acid endoprotease increased rapidly upon regrowth, but its increase preceded the in vitro decline in proteins. The autolytic activity, however, did not change in the early growth stage, and protein had already declined 50% when this activity significantly increased. Electrophoretic separation of intact proteins indicated a substantial shift in relative mobility from higher to lower molecular weights as the season advanced. However, the majority of proteins showed little evidence of net breakdown during early spring growth. Two polypeptides of 38,000 and 56,000 daltons which were present up until mid-April were not detected in extracts of shoots collected in May. The 65% decline in total protein observed by May 10, however, could not be accounted for by the loss of these 2 peptides. It is thus concluded that the immediate requirement of nitrogen at the early stage of growth (up to silver-tip) is met by the transport of soluble nitrogen present in adjacent bark and that redistributed from the wood. The large requirement of nitrogen after silver-tip stage of growth is then met by massive breakdown of storage proteins." @default.
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- W4300792798 date "1982-03-01" @default.
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- W4300792798 title "Mobilization and Metabolism of Protein and Soluble Nitrogen during Spring Growth of Apple Trees" @default.
- W4300792798 doi "https://doi.org/10.21273/jashs.107.2.209" @default.
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