Matches in SemOpenAlex for { <https://semopenalex.org/work/W4300797435> ?p ?o ?g. }
Showing items 1 to 64 of
64
with 100 items per page.
- W4300797435 endingPage "9" @default.
- W4300797435 startingPage "1044" @default.
- W4300797435 abstract "Since the first successful application of somatic cell fusion by Kohler and Milstein to generate hybridomas secreting monoclonal antibodies (Mabs), this hybridoma technique has been widely applied for production of Mabs against a wide variety of antigens. In the lecture, general procedures for establishment of hybridomas and characteristic properties of Mabs were briefly explained and followed by presentation of our clinical studies using Mabs produced in our laboratory to hCG, sperm and tumor cells. 1. Monoclonal antibodies to hCG. Three hybridomas (5D4, 6E4, 2F8) were established by fusing mouse myeloma cells (P3U1) with spleen cells from BALB/c mice immunized with partially purified hCG. Mabs were obtained either from hybridoma culture media or from ascites of mice inoculated the hybridoma cells intraperitoneally. Three Mabs obtained were all IgG1 and each had a unique binding specificity to hCG, hCG-beta, hCG-alpha and LH. They were applied for development of specific, sensitive and easy to perform hCG assays. A reverse passive hemagglutination assay of urinary hCG with a sensitivity of 12.5 IU/l and a sandwich-enzyme immunoassay of serum hCG with a sensitivity of 0.1 mIU/ml were presented. 2. Monoclonal antibodies to human spermatozoa. For elucidation of mechanisms for induction of auto- or iso-sperm immunization and infertility by antisperm antibodies, antigen analysis of seminal components was essential. For this purpose, several Mabs with strong sperm immobilizing (SI) and agglutinating (SA) activities were generated in mice, rats and humans and the corresponding antigens were analysed.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
- W4300797435 created "2022-10-04" @default.
- W4300797435 creator A5029761590 @default.
- W4300797435 date "1988-08-01" @default.
- W4300797435 modified "2023-10-03" @default.
- W4300797435 title "[Monoclonal antibodies]." @default.
- W4300797435 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3075226" @default.
- W4300797435 hasPublicationYear "1988" @default.
- W4300797435 type Work @default.
- W4300797435 citedByCount "0" @default.
- W4300797435 crossrefType "journal-article" @default.
- W4300797435 hasAuthorship W4300797435A5029761590 @default.
- W4300797435 hasConcept C147483822 @default.
- W4300797435 hasConcept C153911025 @default.
- W4300797435 hasConcept C159047783 @default.
- W4300797435 hasConcept C159654299 @default.
- W4300797435 hasConcept C163864487 @default.
- W4300797435 hasConcept C185592680 @default.
- W4300797435 hasConcept C203014093 @default.
- W4300797435 hasConcept C2778502174 @default.
- W4300797435 hasConcept C2780420688 @default.
- W4300797435 hasConcept C2780931953 @default.
- W4300797435 hasConcept C34343649 @default.
- W4300797435 hasConcept C542903549 @default.
- W4300797435 hasConcept C54355233 @default.
- W4300797435 hasConcept C81885089 @default.
- W4300797435 hasConcept C86803240 @default.
- W4300797435 hasConcept C92488253 @default.
- W4300797435 hasConceptScore W4300797435C147483822 @default.
- W4300797435 hasConceptScore W4300797435C153911025 @default.
- W4300797435 hasConceptScore W4300797435C159047783 @default.
- W4300797435 hasConceptScore W4300797435C159654299 @default.
- W4300797435 hasConceptScore W4300797435C163864487 @default.
- W4300797435 hasConceptScore W4300797435C185592680 @default.
- W4300797435 hasConceptScore W4300797435C203014093 @default.
- W4300797435 hasConceptScore W4300797435C2778502174 @default.
- W4300797435 hasConceptScore W4300797435C2780420688 @default.
- W4300797435 hasConceptScore W4300797435C2780931953 @default.
- W4300797435 hasConceptScore W4300797435C34343649 @default.
- W4300797435 hasConceptScore W4300797435C542903549 @default.
- W4300797435 hasConceptScore W4300797435C54355233 @default.
- W4300797435 hasConceptScore W4300797435C81885089 @default.
- W4300797435 hasConceptScore W4300797435C86803240 @default.
- W4300797435 hasConceptScore W4300797435C92488253 @default.
- W4300797435 hasIssue "8" @default.
- W4300797435 hasLocation W43007974351 @default.
- W4300797435 hasOpenAccess W4300797435 @default.
- W4300797435 hasPrimaryLocation W43007974351 @default.
- W4300797435 hasRelatedWork W1509901471 @default.
- W4300797435 hasRelatedWork W1943797027 @default.
- W4300797435 hasRelatedWork W2080261137 @default.
- W4300797435 hasRelatedWork W2162319806 @default.
- W4300797435 hasRelatedWork W2361653128 @default.
- W4300797435 hasRelatedWork W2376080177 @default.
- W4300797435 hasRelatedWork W2388502520 @default.
- W4300797435 hasRelatedWork W2565354684 @default.
- W4300797435 hasRelatedWork W3097899646 @default.
- W4300797435 hasRelatedWork W52564546 @default.
- W4300797435 hasVolume "40" @default.
- W4300797435 isParatext "false" @default.
- W4300797435 isRetracted "false" @default.
- W4300797435 workType "article" @default.