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• W4300873630 endingPage "2359" @default.
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• W4300873630 abstract "P2X receptors are a family of ATP-gated ion channels thought to have intracellular N and C termini and two transmembrane segments separating a large extracellular domain. We examined the involvement of the second putative transmembrane domain (TM2) of the P2X 2 subunit in ion conduction, using the substituted cysteine accessibility method (SCAM). This method tests the ability of hydrophilic reagents such as Ag + or the methanethiosulfonates to modify covalently the sulfhydryl side chains exposed to aqueous environments. ATP-gated current was measured in HEK293 cells transiently expressing either wild-type or functional mutant P2X 2 receptors containing a cysteine substitution in or around TM2. Application of Ag + to gating channels had no sustained effect on wild-type P2X 2 (WT) but irreversibly altered whole-cell currents in 15 mutants. By contrast, bath application of (2-aminoethyl)methanethiosulfonate (MTSEA) to closed channels inhibited 8 of the 15 residues affected by Ag + when the channel was gating. Inhibition of the closed channel was prevented in seven of eight mutants when membrane-permeant MTSEA was scavenged by 20 m m intracellular cysteine, indicating that these seven mutants lie on the intracellular side of the channel gate. Further, MTSEA inhibited current through G342C in the absence of intracellular cysteine but augmented the current when cysteine was present, suggesting that this residue may be part of the gate. Taken together, the data help to the identify a functional domain of the channel pore by mapping residues on either side of the channel gate." @default.
• W4300873630 created "2022-10-04" @default.
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• W4300873630 date "1998-04-01" @default.
• W4300873630 modified "2023-10-13" @default.
• W4300873630 title "A Domain Contributing to the Ion Channel of ATP-Gated P2X₂Receptors Identified by the Substituted Cysteine Accessibility Method" @default.
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• W4300873630 doi "https://doi.org/10.1523/jneurosci.18-07-02350.1998" @default.
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