FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4300961982> ?p ?o ?g. }

• W4300961982 abstract "Over the past few decades, essential role of glycosylation in protein functioning has become widely recognized, rapidly advancing glycan analysis techniques. Because free glycan's lack chromophore or fluorophore properties, and do not ionize well, they are often derivatized to facilitate their separation or detection, and to enhance the sensitivity of the analysis. Released glycan's are usually derivatized using a fluorescent tag, which enables their optical detection in LC profiling. Some fluorescent labels can also promote ionization efficiency, thus facilitating MS detection. For this reason, there is a need to design fluorophores that will contribute more to the fluorescence and ionization of glycan's and the need to quantify these contributions to improve glycan analysis methods. In this paper we focused on negative MS mode as these methods are more informative than methods involving positive MS mode, allowing for a less ambiguous elucidation of detailed glycan structures. Additionally, traditional glycan labels in negative mode MS usually result with diminished sensitivity compared to positive mode, thus making selection of appropriate label even more important for successful high-throughput analysis. Therefore, eleven fluorescent labels of different chemo-physical properties were chosen to study the influence of label hydrophobicity and presence of a negative charge on glycan ionization in negative MS mode. N-glycans released from IgG sample were labeled with one of the eleven labels, purified with HILIC-SPE and analyzed with HILIC-UPLC-FLR-MS. To make evaluation of studied labels performance more objective, analysis was performed in two laboratories and at two mobile phase pH (4.4 and 7.4). Although there was a notable trend of more hydrophobic labels having bigger signal intensities in one laboratory, we observed no such trend in the other laboratory. The results show that MS parameters and intrinsic configuration of the spectrometer have even bigger effect on the final ESI response of the labeled-glycan ionization in negative MS mode that the labels themselves. With this in mind, further research and development of fluorophores that will be suitable for high-throughput glycan analysis in the negative MS mode are proposed." @default.
• W4300961982 created "2022-10-04" @default.
• W4300961982 creator A5003222189 @default.
• W4300961982 creator A5016169931 @default.
• W4300961982 creator A5023163067 @default.
• W4300961982 creator A5030718200 @default.
• W4300961982 creator A5051077634 @default.
• W4300961982 creator A5064938738 @default.
• W4300961982 date "2022-10-03" @default.
• W4300961982 modified "2023-09-25" @default.
• W4300961982 title "In a pursuit of optimal glycan fluorescent label for negative MS mode for high-throughput N-glycan analysis" @default.
• W4300961982 cites W1967544219 @default.
• W4300961982 cites W1979268601 @default.
• W4300961982 cites W1997481671 @default.
• W4300961982 cites W2001105105 @default.
• W4300961982 cites W2007236926 @default.
• W4300961982 cites W2016717705 @default.
• W4300961982 cites W2023509065 @default.
• W4300961982 cites W2026096535 @default.
• W4300961982 cites W2027116733 @default.
• W4300961982 cites W2034564275 @default.
• W4300961982 cites W2045093966 @default.
• W4300961982 cites W2052810090 @default.
• W4300961982 cites W2075166329 @default.
• W4300961982 cites W2085475969 @default.
• W4300961982 cites W2099532264 @default.
• W4300961982 cites W2140723605 @default.
• W4300961982 cites W2151338692 @default.
• W4300961982 cites W2342425351 @default.
• W4300961982 cites W2557582001 @default.
• W4300961982 cites W2564497310 @default.
• W4300961982 cites W2593436234 @default.
• W4300961982 cites W2763231564 @default.
• W4300961982 cites W2794525690 @default.
• W4300961982 cites W2804536953 @default.
• W4300961982 cites W2884507766 @default.
• W4300961982 cites W2973145629 @default.
• W4300961982 cites W3018150932 @default.
• W4300961982 cites W3082292819 @default.
• W4300961982 cites W3134877569 @default.
• W4300961982 cites W3158311560 @default.
• W4300961982 cites W3159475428 @default.
• W4300961982 cites W3196620837 @default.
• W4300961982 cites W3198927554 @default.
• W4300961982 cites W3201103902 @default.
• W4300961982 cites W4284891445 @default.
• W4300961982 doi "https://doi.org/10.3389/fchem.2022.999770" @default.
• W4300961982 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36262345" @default.
• W4300961982 hasPublicationYear "2022" @default.
• W4300961982 type Work @default.
• W4300961982 citedByCount "0" @default.
• W4300961982 crossrefType "journal-article" @default.
• W4300961982 hasAuthorship W4300961982A5003222189 @default.
• W4300961982 hasAuthorship W4300961982A5016169931 @default.
• W4300961982 hasAuthorship W4300961982A5023163067 @default.
• W4300961982 hasAuthorship W4300961982A5030718200 @default.
• W4300961982 hasAuthorship W4300961982A5051077634 @default.
• W4300961982 hasAuthorship W4300961982A5064938738 @default.
• W4300961982 hasBestOaLocation W43009619821 @default.
• W4300961982 hasConcept C108625454 @default.
• W4300961982 hasConcept C116817701 @default.
• W4300961982 hasConcept C121332964 @default.
• W4300961982 hasConcept C179998833 @default.
• W4300961982 hasConcept C185592680 @default.
• W4300961982 hasConcept C206212055 @default.
• W4300961982 hasConcept C2777313579 @default.
• W4300961982 hasConcept C2778617687 @default.
• W4300961982 hasConcept C43617362 @default.
• W4300961982 hasConcept C55493867 @default.
• W4300961982 hasConcept C62520636 @default.
• W4300961982 hasConcept C91881484 @default.
• W4300961982 hasConceptScore W4300961982C108625454 @default.
• W4300961982 hasConceptScore W4300961982C116817701 @default.
• W4300961982 hasConceptScore W4300961982C121332964 @default.
• W4300961982 hasConceptScore W4300961982C179998833 @default.
• W4300961982 hasConceptScore W4300961982C185592680 @default.
• W4300961982 hasConceptScore W4300961982C206212055 @default.
• W4300961982 hasConceptScore W4300961982C2777313579 @default.
• W4300961982 hasConceptScore W4300961982C2778617687 @default.
• W4300961982 hasConceptScore W4300961982C43617362 @default.
• W4300961982 hasConceptScore W4300961982C55493867 @default.
• W4300961982 hasConceptScore W4300961982C62520636 @default.
• W4300961982 hasConceptScore W4300961982C91881484 @default.
• W4300961982 hasLocation W43009619821 @default.
• W4300961982 hasLocation W43009619822 @default.
• W4300961982 hasLocation W43009619823 @default.
• W4300961982 hasOpenAccess W4300961982 @default.
• W4300961982 hasPrimaryLocation W43009619821 @default.
• W4300961982 hasRelatedWork W2008102925 @default.
• W4300961982 hasRelatedWork W2066144238 @default.
• W4300961982 hasRelatedWork W2323799614 @default.
• W4300961982 hasRelatedWork W3085174883 @default.
• W4300961982 hasRelatedWork W3202392152 @default.
• W4300961982 hasRelatedWork W4234135372 @default.
• W4300961982 hasRelatedWork W4307430670 @default.
• W4300961982 hasRelatedWork W4313208229 @default.
• W4300961982 hasRelatedWork W9804831 @default.
• W4300961982 hasRelatedWork W2460880973 @default.
• W4300961982 hasVolume "10" @default.
• W4300961982 isParatext "false" @default.

• next