FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4300972466> ?p ?o ?g. }

• W4300972466 abstract "Microbial cocultures are used as a tool to stimulate natural product biosynthesis. However, studies often empirically combine different organisms without a deeper understanding of the population dynamics. As filamentous organisms offer a vast metabolic diversity, we developed a model filamentous coculture of the cellulolytic fungus Trichoderma reesei RUT-C30 and the noncellulolytic bacterium Streptomyces coelicolor A3(2). The coculture was set up to use α-cellulose as a carbon source. This established a dependency of S. coelicolor on hydrolysate sugars released by T. reesei cellulases. To provide detailed insight into coculture dynamics, we applied high-throughput online monitoring of the respiration rate and fluorescence of the tagged strains. The respiration rate allowed us to distinguish the conditions of successful cellulase formation. Furthermore, to dissect the individual strain contributions, T. reesei and S. coelicolor were tagged with mCherry and mNeonGreen (mNG) fluorescence proteins, respectively. When evaluating varying inoculation ratios, it was observed that both partners outcompete the other when given a high inoculation advantage. Nonetheless, adequate proportions for simultaneous growth of both partners, cellulase, and pigment production could be determined. Finally, population dynamics were also tuned by modulating abiotic factors. Increased osmolality provided a growth advantage to S. coelicolor. In contrast, an increase in shaking frequency had a negative effect on S. coelicolor biomass formation, promoting T. reesei. This comprehensive analysis fills important knowledge gaps in the control of complex cocultures and accelerates the setup of other tailor-made coculture bioprocesses." @default.
• W4300972466 created "2022-10-04" @default.
• W4300972466 creator A5000958649 @default.
• W4300972466 creator A5008653078 @default.
• W4300972466 creator A5016954737 @default.
• W4300972466 creator A5022603631 @default.
• W4300972466 creator A5057532506 @default.
• W4300972466 creator A5064980320 @default.
• W4300972466 date "2022-10-01" @default.
• W4300972466 modified "2023-10-13" @default.
• W4300972466 title "Tunable population dynamics in a synthetic filamentous coculture" @default.
• W4300972466 cites W1599536643 @default.
• W4300972466 cites W1982842330 @default.
• W4300972466 cites W2005762209 @default.
• W4300972466 cites W2012960603 @default.
• W4300972466 cites W2039975552 @default.
• W4300972466 cites W2055745032 @default.
• W4300972466 cites W2080457370 @default.
• W4300972466 cites W2094468599 @default.
• W4300972466 cites W2097655674 @default.
• W4300972466 cites W2105288771 @default.
• W4300972466 cites W2106618245 @default.
• W4300972466 cites W2106943551 @default.
• W4300972466 cites W2116663774 @default.
• W4300972466 cites W2123610581 @default.
• W4300972466 cites W2127681878 @default.
• W4300972466 cites W2128635872 @default.
• W4300972466 cites W2137402060 @default.
• W4300972466 cites W2138468730 @default.
• W4300972466 cites W2142314320 @default.
• W4300972466 cites W2145981596 @default.
• W4300972466 cites W2152826723 @default.
• W4300972466 cites W2178324140 @default.
• W4300972466 cites W2210426368 @default.
• W4300972466 cites W2526106576 @default.
• W4300972466 cites W2531072799 @default.
• W4300972466 cites W2545950570 @default.
• W4300972466 cites W2588828888 @default.
• W4300972466 cites W2608089746 @default.
• W4300972466 cites W2735752608 @default.
• W4300972466 cites W2740403323 @default.
• W4300972466 cites W2790317240 @default.
• W4300972466 cites W2800780732 @default.
• W4300972466 cites W2904282688 @default.
• W4300972466 cites W2944231932 @default.
• W4300972466 cites W2999177106 @default.
• W4300972466 cites W3000153187 @default.
• W4300972466 cites W3005299705 @default.
• W4300972466 cites W3031048167 @default.
• W4300972466 cites W3044450297 @default.
• W4300972466 cites W3046660098 @default.
• W4300972466 cites W3083248956 @default.
• W4300972466 cites W3092007920 @default.
• W4300972466 cites W3107114247 @default.
• W4300972466 cites W3128102021 @default.
• W4300972466 cites W3183557051 @default.
• W4300972466 cites W3193286519 @default.
• W4300972466 cites W3198308611 @default.
• W4300972466 cites W4206124553 @default.
• W4300972466 cites W4210938022 @default.
• W4300972466 cites W4224254743 @default.
• W4300972466 cites W4225015755 @default.
• W4300972466 cites W4255518991 @default.
• W4300972466 cites W4291989548 @default.
• W4300972466 cites W4293247451 @default.
• W4300972466 doi "https://doi.org/10.1002/mbo3.1324" @default.
• W4300972466 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36314761" @default.
• W4300972466 hasPublicationYear "2022" @default.
• W4300972466 type Work @default.
• W4300972466 citedByCount "4" @default.
• W4300972466 countsByYear W43009724662023 @default.
• W4300972466 crossrefType "journal-article" @default.
• W4300972466 hasAuthorship W4300972466A5000958649 @default.
• W4300972466 hasAuthorship W4300972466A5008653078 @default.
• W4300972466 hasAuthorship W4300972466A5016954737 @default.
• W4300972466 hasAuthorship W4300972466A5022603631 @default.
• W4300972466 hasAuthorship W4300972466A5057532506 @default.
• W4300972466 hasAuthorship W4300972466A5064980320 @default.
• W4300972466 hasBestOaLocation W43009724661 @default.
• W4300972466 hasConcept C104317684 @default.
• W4300972466 hasConcept C141603559 @default.
• W4300972466 hasConcept C143065580 @default.
• W4300972466 hasConcept C144024400 @default.
• W4300972466 hasConcept C149923435 @default.
• W4300972466 hasConcept C185592680 @default.
• W4300972466 hasConcept C2776125036 @default.
• W4300972466 hasConcept C2779251873 @default.
• W4300972466 hasConcept C2780681812 @default.
• W4300972466 hasConcept C2908647359 @default.
• W4300972466 hasConcept C31903555 @default.
• W4300972466 hasConcept C55493867 @default.
• W4300972466 hasConcept C86803240 @default.
• W4300972466 hasConcept C89423630 @default.
• W4300972466 hasConceptScore W4300972466C104317684 @default.
• W4300972466 hasConceptScore W4300972466C141603559 @default.
• W4300972466 hasConceptScore W4300972466C143065580 @default.
• W4300972466 hasConceptScore W4300972466C144024400 @default.
• W4300972466 hasConceptScore W4300972466C149923435 @default.
• W4300972466 hasConceptScore W4300972466C185592680 @default.
• W4300972466 hasConceptScore W4300972466C2776125036 @default.

• next