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- W4301035768 abstract "ABSTRACT Tadpoles of Xenopus laevis completely lacking primordial germ cells were obtained by irradiating the vegetal hemisphere of early 2-cell eggs with u.v. (wavelength, 253·7 nm; dose, ca. 6000 ergs/mm2). An increasing number of primordial germ cells were observed as the stage at irradiation advanced from early 2-cell to early 4-cell stages. Furthermore, early 2-cell eggs irradiated with doses ranging from 750 to 6000 ergs/mm2 grew into tadpoles carrying a decreasing number of primordial germ cells in accord with the increase of the dose. On the other hand, tadpoles developed from eggs irradiated immediately after being centrifuged at 150 g for 1 min at early 2-cell stage to displace the ‘germinal plasm’ deeper into the cytoplasm, carried a considerable number of primordial germ cells. These facts were interpreted to suggest the presence of u.v.-sensitive germ cell determinant in the ‘germinal plasm’. It was revealed by varying the area of irradiation that the number of primordial germ cells decreased in direct proportion to the increase of the area irradiated. It was concluded that the amount of the u.v.-sensitive material(s) contained in the ‘germinal plasm’ determined the number of primordial germ cells in tadpoles." @default.
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- W4301035768 date "1974-01-01" @default.
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- W4301035768 title "Relationship between the amount of the ‘germinal plasm’ and the number of primordial germ cells in <i>Xenopus laevis</i>" @default.
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- W4301035768 doi "https://doi.org/10.1242/dev.31.1.89" @default.
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