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• W4301183087 abstract "Abstract Background Neuropathic pain is still a challenge for clinical treatment as a result of the comprehensive pathogenesis. Although emerging evidence demonstrates the pivotal role of glial cells in regulating neuropathic pain, the role of Schwann cells and their underlying mechanisms still need to be uncovered. Pannexin 1 (Panx 1), an important membrane channel for the release of ATP and inflammatory cytokines, as well as its activation in central glial cells, contributes to pain development. Here, we hypothesized that Schwann cell Panx 1 participates in the regulation of neuroinflammation and contributes to neuropathic pain. Methods A mouse model of chronic constriction injury (CCI) in CD1 adult mice or P0-Cre transgenic mice, and in vitro cultured Schwann cells were used. Intrasciatic injection with Panx 1 blockers or the desired virus was used to knock down the expression of Panx 1. Mechanical and thermal sensitivity was assessed using Von Frey and a hot plate assay. The expression of Panx 1 was measured using qPCR, western blotting, and immunofluorescence. The production of cytokines was monitored through qPCR and enzyme-linked immunosorbent assay (ELISA). Panx1 channel activity was detected by ethidium bromide (EB) uptake. Results CCI induced persistent neuroinflammatory responses and upregulation of Panx 1 in Schwann cells. Intrasciatic injection of Panx 1 blockers, carbenoxolone (CBX), probenecid, and Panx 1 mimetic peptide ( 10 Panx) effectively reduced mechanical and heat hyperalgesia. Probenecid treatment of CCI-induced mice significantly reduced Panx 1 expression in Schwann cells, but not in dorsal root ganglion (DRG). In addition, Panx 1 knockdown in Schwann cells with Panx 1 shRNA-AAV in P0-Cre mice significantly reduced CCI-induced neuropathic pain. To determine whether Schwann cell Panx 1 participates in the regulation of neuroinflammation and contributes to neuropathic pain, we evaluated its effect in LPS-treated Schwann cells. We found that inhibition of Panx 1 via CBX and Panx 1-siRNA effectively attenuated the production of selective cytokines, as well as its mechanism of action being dependent on both Panx 1 channel activity and its expression. Conclusion In this study, we found that CCI-related neuroinflammation correlates with Panx 1 activation in Schwann cells, indicating that inhibition of Panx 1 channels in Schwann cells reduces neuropathic pain through the suppression of neuroinflammatory responses." @default.
• W4301183087 created "2022-10-04" @default.
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• W4301183087 date "2022-10-04" @default.
• W4301183087 modified "2023-10-09" @default.
• W4301183087 title "Inhibition of Schwann cell pannexin 1 attenuates neuropathic pain through the suppression of inflammatory responses" @default.
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• W4301183087 cites W1919181290 @default.
• W4301183087 cites W1951273690 @default.
• W4301183087 cites W1974103263 @default.
• W4301183087 cites W1982322107 @default.
• W4301183087 cites W1983355789 @default.
• W4301183087 cites W1993285878 @default.
• W4301183087 cites W2005067021 @default.
• W4301183087 cites W2015196837 @default.
• W4301183087 cites W2016560481 @default.
• W4301183087 cites W2018978435 @default.
• W4301183087 cites W2019919875 @default.
• W4301183087 cites W2024660851 @default.
• W4301183087 cites W2046296795 @default.
• W4301183087 cites W2047136668 @default.
• W4301183087 cites W2048102871 @default.
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• W4301183087 cites W2094690206 @default.
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• W4301183087 cites W2114855930 @default.
• W4301183087 cites W2127049690 @default.
• W4301183087 cites W2134557769 @default.
• W4301183087 cites W2137650873 @default.
• W4301183087 cites W2154507501 @default.
• W4301183087 cites W2342915142 @default.
• W4301183087 cites W2409683891 @default.
• W4301183087 cites W2534023149 @default.
• W4301183087 cites W2547814918 @default.
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• W4301183087 cites W2578885464 @default.
• W4301183087 cites W2582756289 @default.
• W4301183087 cites W2588140376 @default.
• W4301183087 cites W2604463267 @default.
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• W4301183087 cites W2770446525 @default.
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• W4301183087 cites W2799442274 @default.
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• W4301183087 doi "https://doi.org/10.1186/s12974-022-02603-x" @default.
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