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- W4301351256 abstract "Newer discovery of Salmonella strains warranted the development of newer ELISA system. Theaim of this works is to develop a sensitive and simple indirect ELISA for the detection ofSalmonella Typhimurium using formalin- and heat-killed cells as immunogens. The parameters ofthe resultant calibration curve for the formalin-killed cells (FKC) were minimum absorbance of0.101± 0.011, maximum absorbance of 2.804± 0.020, slope parameter of 1.197, while c, theconcentration that results in 50% response was 4.22 x 106 CFU/ml. The LOD and LOQ were19,300 and 52,700 CFU/mL, respectively. The parameters of the typical calibration curve forheat-killed cells (HKC) were minimum absorbance of 0.115± 0.013, maximum absorbance of2.810± 0.021, slope parameter was 1.441, while c, the concentration that results in 50% responsewas 2.15 x 106 CFU/ml. The LOD and LOQ were 47,100 and 123,000 CFU/mL, respectively.Antibody raised to FKC was twice the sensitivity and approximately ten times the linear rangecompared to antibody raised to HKC. Antibody produced from formalin-killed cells exhibitedlower cross reactivity than heat-killed cells. The results of this work could open new avenues onoptimization of antigen preparation for maximal sensitivity of the developed polyclonal antibody." @default.
- W4301351256 created "2022-10-05" @default.
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- W4301351256 date "2014-07-31" @default.
- W4301351256 modified "2023-09-27" @default.
- W4301351256 title "Comparison of the sensitivity and cross-reactivity between heat-killed and formalin-killed cells during the development of indirect competitive ELISA for the detection of Salmonella Typhimurium" @default.
- W4301351256 doi "https://doi.org/10.54987/jebat.v2i1.144" @default.
- W4301351256 hasPublicationYear "2014" @default.
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