FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4301431071> ?p ?o ?g. }

• W4301431071 abstract "Electron radiation damage to macromolecules is an inevitable resolution limit factor in all major structural determination applications using cryo-electron microscopy (cryo-EM). Single particle analysis (SPA) and micro-crystal electron diffraction (MicroED) have been employed to assess radiation damage with a variety of protein complexes. Although radiation induced sidechain density loss and resolution decay were observed by both methods, the minimum dose of electron irradiation reducing high-resolution limit reported by SPA is more than ten folds higher than measured by MicroED using the conventional dose concept, and there is a gap between the attained resolutions assessed by these two methods. We compared and analyzed these two approaches side-by-side in detail from several aspects to identify some crucial determinants and to explain this discrepancy. Probability of a high energy electron being inelastically scattered by a macromolecule is proportional to number of layers of the molecules in its transmission path. As a result, the same electron dose could induce much more site-specific damage to macromolecules in 3D protein crystal than single particle samples. Major differences in data collection and processing scheme are the key factors to different levels of sensitivity to radiation damage at high resolution between the two methods. High resolution electron diffraction in MicroED dataset is very sensitive to global damage to 3D protein crystals with low dose accumulation, and its intensity attenuation rates at atomic resolution shell could be applied for estimating ratio of damaged and total selected single particles for SPA. More in-depth systematically radiation damage assessments using SPA and MicroED will benefit all applications of cryo-EM, especially cellular structure analysis by tomography." @default.
• W4301431071 created "2022-10-05" @default.
• W4301431071 creator A5001710213 @default.
• W4301431071 creator A5021583015 @default.
• W4301431071 date "2022-10-05" @default.
• W4301431071 modified "2023-10-14" @default.
• W4301431071 title "Analysis and comparison of electron radiation damage assessments in Cryo-EM by single particle analysis and micro-crystal electron diffraction" @default.
• W4301431071 cites W1781789881 @default.
• W4301431071 cites W1810581179 @default.
• W4301431071 cites W1980885982 @default.
• W4301431071 cites W198293660 @default.
• W4301431071 cites W1983126883 @default.
• W4301431071 cites W1989784263 @default.
• W4301431071 cites W1992742630 @default.
• W4301431071 cites W1994124107 @default.
• W4301431071 cites W2002547620 @default.
• W4301431071 cites W2005601437 @default.
• W4301431071 cites W2021064626 @default.
• W4301431071 cites W2022390988 @default.
• W4301431071 cites W2029360750 @default.
• W4301431071 cites W2029490760 @default.
• W4301431071 cites W2038089138 @default.
• W4301431071 cites W2058548853 @default.
• W4301431071 cites W2061144537 @default.
• W4301431071 cites W2061281811 @default.
• W4301431071 cites W2067374400 @default.
• W4301431071 cites W2073149987 @default.
• W4301431071 cites W2078639536 @default.
• W4301431071 cites W2081550487 @default.
• W4301431071 cites W2087978946 @default.
• W4301431071 cites W2093650342 @default.
• W4301431071 cites W2094719027 @default.
• W4301431071 cites W2131378428 @default.
• W4301431071 cites W2134980892 @default.
• W4301431071 cites W2138233159 @default.
• W4301431071 cites W2141158534 @default.
• W4301431071 cites W2143205611 @default.
• W4301431071 cites W2146801942 @default.
• W4301431071 cites W2148941629 @default.
• W4301431071 cites W2149360962 @default.
• W4301431071 cites W2149705812 @default.
• W4301431071 cites W2158023688 @default.
• W4301431071 cites W2160108669 @default.
• W4301431071 cites W2163265529 @default.
• W4301431071 cites W2427061751 @default.
• W4301431071 cites W2593511418 @default.
• W4301431071 cites W2793257932 @default.
• W4301431071 cites W2901198387 @default.
• W4301431071 cites W2905153860 @default.
• W4301431071 cites W2912891417 @default.
• W4301431071 cites W2942522093 @default.
• W4301431071 cites W3006716706 @default.
• W4301431071 cites W3093646295 @default.
• W4301431071 cites W3094051077 @default.
• W4301431071 cites W3138873101 @default.
• W4301431071 cites W3142815153 @default.
• W4301431071 cites W3202179996 @default.
• W4301431071 cites W4200311841 @default.
• W4301431071 doi "https://doi.org/10.3389/fmolb.2022.988928" @default.
• W4301431071 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36275612" @default.
• W4301431071 hasPublicationYear "2022" @default.
• W4301431071 type Work @default.
• W4301431071 citedByCount "0" @default.
• W4301431071 crossrefType "journal-article" @default.
• W4301431071 hasAuthorship W4301431071A5001710213 @default.
• W4301431071 hasAuthorship W4301431071A5021583015 @default.
• W4301431071 hasBestOaLocation W43014310711 @default.
• W4301431071 hasConcept C111337013 @default.
• W4301431071 hasConcept C111368507 @default.
• W4301431071 hasConcept C120665830 @default.
• W4301431071 hasConcept C121332964 @default.
• W4301431071 hasConcept C127313418 @default.
• W4301431071 hasConcept C138268822 @default.
• W4301431071 hasConcept C147120987 @default.
• W4301431071 hasConcept C153385146 @default.
• W4301431071 hasConcept C153428861 @default.
• W4301431071 hasConcept C154945302 @default.
• W4301431071 hasConcept C178790620 @default.
• W4301431071 hasConcept C185544564 @default.
• W4301431071 hasConcept C185592680 @default.
• W4301431071 hasConcept C192562407 @default.
• W4301431071 hasConcept C20702342 @default.
• W4301431071 hasConcept C207114421 @default.
• W4301431071 hasConcept C2778517922 @default.
• W4301431071 hasConcept C2779345167 @default.
• W4301431071 hasConcept C41008148 @default.
• W4301431071 hasConcept C41999313 @default.
• W4301431071 hasConcept C55493867 @default.
• W4301431071 hasConcept C65293305 @default.
• W4301431071 hasConcept C77557913 @default.
• W4301431071 hasConcept C93877712 @default.
• W4301431071 hasConceptScore W4301431071C111337013 @default.
• W4301431071 hasConceptScore W4301431071C111368507 @default.
• W4301431071 hasConceptScore W4301431071C120665830 @default.
• W4301431071 hasConceptScore W4301431071C121332964 @default.
• W4301431071 hasConceptScore W4301431071C127313418 @default.
• W4301431071 hasConceptScore W4301431071C138268822 @default.
• W4301431071 hasConceptScore W4301431071C147120987 @default.
• W4301431071 hasConceptScore W4301431071C153385146 @default.
• W4301431071 hasConceptScore W4301431071C153428861 @default.

• next