Matches in SemOpenAlex for { <https://semopenalex.org/work/W4301446985> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W4301446985 abstract "Detrusor smooth muscle (DSM) cells present within the urinary bladder wall ultimately facilitate urine storage and voiding. Preparation of the viable, fresh, and isolated DSM cells presents an important technical challenge whose achievement provides optimal cells for subsequent functional and molecular studies. The method developed and elaborated herein, successfully used by our group for over a decade, describes dissection of human urinary bladder specimens obtained from open bladder surgeries followed by an enzymatic two-step treatment of DSM pieces and mechanical trituration to obtain freshly isolated DSM cells. The initial step involves dissection to separate the DSM layer (also known as muscularis propria) from mucosa (urothelium, lamina propria, and muscularis mucosa) and the adjacent connective, vascular, and adipose tissues present. The DSM is then cut into pieces (2-3 mm x 4-6 mm) in nominal Ca2+-containing dissection/digestion solution (DS). DSM pieces are next transferred to and sequentially treated separately with DS containing papain and collagenase at ~37 °C for 30-45 min per step. Following washes with DS containing enzyme-free bovine serum and trituration with a fire-polished pipette, the pieces release single DSM cells. Freshly isolated DSM cells are ideally suited for patch-clamp electrophysiological and pharmacological characterizations of ion channels. Specifically, we show that the TRPM4 channel blocker 9-phenanthrol reduces voltage-step evoked cation currents recorded with the amphotericin-B perforated patch-clamp approach. DSM cells can also be studied by other techniques such as single cell RT-PCR, microarray analysis, immunocytochemistry, in situ proximity ligation assay, and Ca2+ imaging. The main advantage of utilizing single DSM cells is that the observations made relate directly to single cell characteristics revealed. Studies of freshly isolated human DSM cells have provided important insights characterizing the properties of various ion channels including cation-permeable in the urinary bladder and will continue as a gold standard in elucidating DSM cellular properties and regulatory mechanisms." @default.
- W4301446985 created "2022-10-05" @default.
- W4301446985 creator A5036686407 @default.
- W4301446985 creator A5051518430 @default.
- W4301446985 creator A5073315335 @default.
- W4301446985 creator A5091902641 @default.
- W4301446985 date "2020-01-31" @default.
- W4301446985 modified "2023-09-30" @default.
- W4301446985 title "Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents" @default.
- W4301446985 doi "https://doi.org/10.3791/59884-v" @default.
- W4301446985 hasPublicationYear "2020" @default.
- W4301446985 type Work @default.
- W4301446985 citedByCount "0" @default.
- W4301446985 crossrefType "journal-article" @default.
- W4301446985 hasAuthorship W4301446985A5036686407 @default.
- W4301446985 hasAuthorship W4301446985A5051518430 @default.
- W4301446985 hasAuthorship W4301446985A5073315335 @default.
- W4301446985 hasAuthorship W4301446985A5091902641 @default.
- W4301446985 hasBestOaLocation W43014469851 @default.
- W4301446985 hasConcept C105702510 @default.
- W4301446985 hasConcept C12554922 @default.
- W4301446985 hasConcept C142724271 @default.
- W4301446985 hasConcept C147789679 @default.
- W4301446985 hasConcept C147944092 @default.
- W4301446985 hasConcept C151552171 @default.
- W4301446985 hasConcept C153911025 @default.
- W4301446985 hasConcept C170493617 @default.
- W4301446985 hasConcept C181199279 @default.
- W4301446985 hasConcept C185592680 @default.
- W4301446985 hasConcept C2777310793 @default.
- W4301446985 hasConcept C2778394429 @default.
- W4301446985 hasConcept C35866371 @default.
- W4301446985 hasConcept C50254741 @default.
- W4301446985 hasConcept C529295009 @default.
- W4301446985 hasConcept C55493867 @default.
- W4301446985 hasConcept C71924100 @default.
- W4301446985 hasConcept C86803240 @default.
- W4301446985 hasConceptScore W4301446985C105702510 @default.
- W4301446985 hasConceptScore W4301446985C12554922 @default.
- W4301446985 hasConceptScore W4301446985C142724271 @default.
- W4301446985 hasConceptScore W4301446985C147789679 @default.
- W4301446985 hasConceptScore W4301446985C147944092 @default.
- W4301446985 hasConceptScore W4301446985C151552171 @default.
- W4301446985 hasConceptScore W4301446985C153911025 @default.
- W4301446985 hasConceptScore W4301446985C170493617 @default.
- W4301446985 hasConceptScore W4301446985C181199279 @default.
- W4301446985 hasConceptScore W4301446985C185592680 @default.
- W4301446985 hasConceptScore W4301446985C2777310793 @default.
- W4301446985 hasConceptScore W4301446985C2778394429 @default.
- W4301446985 hasConceptScore W4301446985C35866371 @default.
- W4301446985 hasConceptScore W4301446985C50254741 @default.
- W4301446985 hasConceptScore W4301446985C529295009 @default.
- W4301446985 hasConceptScore W4301446985C55493867 @default.
- W4301446985 hasConceptScore W4301446985C71924100 @default.
- W4301446985 hasConceptScore W4301446985C86803240 @default.
- W4301446985 hasIssue "155" @default.
- W4301446985 hasLocation W43014469851 @default.
- W4301446985 hasOpenAccess W4301446985 @default.
- W4301446985 hasPrimaryLocation W43014469851 @default.
- W4301446985 hasRelatedWork W138857511 @default.
- W4301446985 hasRelatedWork W1874483258 @default.
- W4301446985 hasRelatedWork W1992469700 @default.
- W4301446985 hasRelatedWork W2068278617 @default.
- W4301446985 hasRelatedWork W2085624112 @default.
- W4301446985 hasRelatedWork W2088766374 @default.
- W4301446985 hasRelatedWork W2279057577 @default.
- W4301446985 hasRelatedWork W2741067040 @default.
- W4301446985 hasRelatedWork W50277463 @default.
- W4301446985 hasRelatedWork W978851527 @default.
- W4301446985 isParatext "false" @default.
- W4301446985 isRetracted "false" @default.
- W4301446985 workType "article" @default.