Matches in SemOpenAlex for { <https://semopenalex.org/work/W4301453504> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W4301453504 abstract "Regardless of the extraction method, optimized RNA extraction of tissues and cell lines are carried out in four stages: 1) homogenization, 2) effective denaturation of proteins from RNA, 3) ribonuclease inactivation, and 4) removal of contamination from DNA, proteins, and carbohydrates. However, it is very laborious to maintain the integrity of RNA when there are high levels of RNase in the tissue. Spontaneous autolysis makes it very difficult to extract RNA from pancreatic tissue without damaging it. Thus, a practical RNA extraction method is needed to maintain the integrity of pancreatic tissues during the extraction process. An experimental and comparative study of existing protocols was carried out by obtaining 20-30 mg of rat pancreatic tissues in less than 2 minutes and extracting the RNA. The results were assessed by electrophoresis. The experiments were carried out three times for generalization of the results. Immersing pancreatic tissue in RNA stabilization reagent at -80 °C for 24 h yielded high integrity RNA, when the RNA extraction reagent was used as the reagent. The results obtained were comparable to the results obtained from commercial kits with spin column bindings." @default.
- W4301453504 created "2022-10-05" @default.
- W4301453504 creator A5021378446 @default.
- W4301453504 creator A5041976731 @default.
- W4301453504 creator A5076946715 @default.
- W4301453504 creator A5088574806 @default.
- W4301453504 date "2020-09-19" @default.
- W4301453504 modified "2023-09-27" @default.
- W4301453504 title "Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue" @default.
- W4301453504 doi "https://doi.org/10.3791/61255-v" @default.
- W4301453504 hasPublicationYear "2020" @default.
- W4301453504 type Work @default.
- W4301453504 citedByCount "1" @default.
- W4301453504 countsByYear W43014535042023 @default.
- W4301453504 crossrefType "journal-article" @default.
- W4301453504 hasAuthorship W4301453504A5021378446 @default.
- W4301453504 hasAuthorship W4301453504A5041976731 @default.
- W4301453504 hasAuthorship W4301453504A5076946715 @default.
- W4301453504 hasAuthorship W4301453504A5088574806 @default.
- W4301453504 hasConcept C104317684 @default.
- W4301453504 hasConcept C10879258 @default.
- W4301453504 hasConcept C112190416 @default.
- W4301453504 hasConcept C130217890 @default.
- W4301453504 hasConcept C153911025 @default.
- W4301453504 hasConcept C185592680 @default.
- W4301453504 hasConcept C18903297 @default.
- W4301453504 hasConcept C2778722038 @default.
- W4301453504 hasConcept C2778976237 @default.
- W4301453504 hasConcept C2780704907 @default.
- W4301453504 hasConcept C43617362 @default.
- W4301453504 hasConcept C4725764 @default.
- W4301453504 hasConcept C552990157 @default.
- W4301453504 hasConcept C55493867 @default.
- W4301453504 hasConcept C67705224 @default.
- W4301453504 hasConcept C86803240 @default.
- W4301453504 hasConceptScore W4301453504C104317684 @default.
- W4301453504 hasConceptScore W4301453504C10879258 @default.
- W4301453504 hasConceptScore W4301453504C112190416 @default.
- W4301453504 hasConceptScore W4301453504C130217890 @default.
- W4301453504 hasConceptScore W4301453504C153911025 @default.
- W4301453504 hasConceptScore W4301453504C185592680 @default.
- W4301453504 hasConceptScore W4301453504C18903297 @default.
- W4301453504 hasConceptScore W4301453504C2778722038 @default.
- W4301453504 hasConceptScore W4301453504C2778976237 @default.
- W4301453504 hasConceptScore W4301453504C2780704907 @default.
- W4301453504 hasConceptScore W4301453504C43617362 @default.
- W4301453504 hasConceptScore W4301453504C4725764 @default.
- W4301453504 hasConceptScore W4301453504C552990157 @default.
- W4301453504 hasConceptScore W4301453504C55493867 @default.
- W4301453504 hasConceptScore W4301453504C67705224 @default.
- W4301453504 hasConceptScore W4301453504C86803240 @default.
- W4301453504 hasIssue "163" @default.
- W4301453504 hasLocation W43014535041 @default.
- W4301453504 hasOpenAccess W4301453504 @default.
- W4301453504 hasPrimaryLocation W43014535041 @default.
- W4301453504 hasRelatedWork W160489826 @default.
- W4301453504 hasRelatedWork W1966103612 @default.
- W4301453504 hasRelatedWork W1975526725 @default.
- W4301453504 hasRelatedWork W1990124746 @default.
- W4301453504 hasRelatedWork W2010603372 @default.
- W4301453504 hasRelatedWork W2419489608 @default.
- W4301453504 hasRelatedWork W2553671003 @default.
- W4301453504 hasRelatedWork W3088628189 @default.
- W4301453504 hasRelatedWork W4242595528 @default.
- W4301453504 hasRelatedWork W4301453504 @default.
- W4301453504 isParatext "false" @default.
- W4301453504 isRetracted "false" @default.
- W4301453504 workType "article" @default.