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- W4302445520 abstract "ABSTRACT One bank vole (Clethrionomys glareolus) embryo and two mouse embryos were combined at the 8-to 16-blastomere stage and cultured in vitro for 33–47 h. In 66% of cases single regular blastocysts were formed. The chimaeric composition of blastocysts was confirmed karyologically. Out of the 222 blastocysts transplanted to 49 pseudopregnant mouse recipients, a total of 52 implantations were found in 20 recipients. Among the 52 implantations, 14 contained embryos and the remaining were resorptions. The majority of embryos were abnormal and fell into two categories : (1) groups of cells surrounded by Reichert’s membrane and lying freely in a cavity filled with giant trophoblastic cells, (2) small and retarded eggcylinders usually composed of endoderm and ectoderm only, and containing a proamniotic cavity. The ectoplacental cone of these embryos was poorly developed or lacking altogether. Two normal-looking embryos were recovered on the 9th and 10th day (4-somite and ca. 12-somite stage). Chimaerism of the younger embryo was confirmed karyologically. No evidence of chimaerism was available in the case of older embryo which was examined histologically. Thirteen implantations examined between 11th and 17th day contained only resorptions. It is suggested that the main cause of the heavy mortality of chimaeric embryos is the profound difference in the course of embryogenesis of these two species immediately following implantation." @default.
- W4302445520 created "2022-10-06" @default.
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- W4302445520 date "1975-06-01" @default.
- W4302445520 modified "2023-09-27" @default.
- W4302445520 title "Development of mouse−bank vole interspecific chimaeric embryos" @default.
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- W4302445520 doi "https://doi.org/10.1242/dev.33.3.731" @default.
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