FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4303044070> ?p ?o ?g. }

• W4303044070 abstract "Gene expression in pathogenic protozoans of the family Trypanosomatidae has several novel features, including multiple eIF4F-like complexes involved in protein synthesis. The eukaryotic eIF4F complex, formed mainly by eIF4E and eIF4G subunits, is responsible for the canonical selection of mRNAs required for the initiation of mRNA translation. The best-known complexes implicated in translation in trypanosomatids are based on two related pairs of eIF4E and eIF4G subunits (EIF4E3/EIF4G4 and EIF4E4/EIF4G3), whose functional distinctions remain to be fully described. Here, to define interactomes associated with both complexes in Trypanosoma brucei procyclic forms, we performed parallel immunoprecipitation experiments followed by identification of proteins co-precipitated with the four tagged eIF4E and eIF4G subunits. A number of different protein partners, including RNA binding proteins and helicases, specifically co-precipitate with each complex. Highlights with the EIF4E4/EIF4G3 pair include RBP23, PABP1, EIF4AI and the CRK1 kinase. Co-precipitated partners with the EIF4E3/EIF4G4 pair are more diverse and include DRBD2, PABP2 and different zinc-finger proteins and RNA helicases. EIF4E3/EIF4G4 are essential for viability and to better define their role, we further investigated their phenotypes after knockdown. Depletion of either EIF4E3/EIF4G4 mRNAs lead to aberrant morphology with a more direct impact on events associated with cytokinesis. We also sought to identify those mRNAs differentially associated with each complex through CLIP-seq with the two eIF4E subunits. Predominant among EIF4E4-bound transcripts are those encoding ribosomal proteins, absent from those found with EIF4E3, which are generally more diverse. RNAi mediated depletion of EIF4E4, which does not affect proliferation, does not lead to changes in mRNAs or proteins associated with EIF4E3, confirming a lack of redundancy and distinct roles for the two complexes." @default.
• W4303044070 created "2022-10-07" @default.
• W4303044070 creator A5004071005 @default.
• W4303044070 creator A5016942073 @default.
• W4303044070 creator A5019675162 @default.
• W4303044070 creator A5024442281 @default.
• W4303044070 creator A5025270882 @default.
• W4303044070 creator A5031918329 @default.
• W4303044070 creator A5041382399 @default.
• W4303044070 creator A5045517935 @default.
• W4303044070 creator A5054418515 @default.
• W4303044070 creator A5059482371 @default.
• W4303044070 creator A5060383868 @default.
• W4303044070 creator A5065691855 @default.
• W4303044070 creator A5073423283 @default.
• W4303044070 creator A5085411329 @default.
• W4303044070 creator A5087662456 @default.
• W4303044070 date "2022-10-07" @default.
• W4303044070 modified "2023-09-30" @default.
• W4303044070 title "Distinct mRNA and protein interactomes highlight functional differentiation of major eIF4F-like complexes from Trypanosoma brucei" @default.
• W4303044070 cites W1506886301 @default.
• W4303044070 cites W1507213641 @default.
• W4303044070 cites W1533435003 @default.
• W4303044070 cites W1558463482 @default.
• W4303044070 cites W1585976445 @default.
• W4303044070 cites W1786582555 @default.
• W4303044070 cites W1922815468 @default.
• W4303044070 cites W1966116114 @default.
• W4303044070 cites W1978423834 @default.
• W4303044070 cites W1990051139 @default.
• W4303044070 cites W2025007214 @default.
• W4303044070 cites W2029229384 @default.
• W4303044070 cites W2033006340 @default.
• W4303044070 cites W2033425667 @default.
• W4303044070 cites W2043798684 @default.
• W4303044070 cites W2050547705 @default.
• W4303044070 cites W2051817585 @default.
• W4303044070 cites W2052050987 @default.
• W4303044070 cites W2057717261 @default.
• W4303044070 cites W2061642409 @default.
• W4303044070 cites W2070761723 @default.
• W4303044070 cites W2075577819 @default.
• W4303044070 cites W2077569247 @default.
• W4303044070 cites W2078073818 @default.
• W4303044070 cites W2089351045 @default.
• W4303044070 cites W2095256515 @default.
• W4303044070 cites W2105219600 @default.
• W4303044070 cites W2107248794 @default.
• W4303044070 cites W2109700867 @default.
• W4303044070 cites W2112421455 @default.
• W4303044070 cites W2113588758 @default.
• W4303044070 cites W2120937476 @default.
• W4303044070 cites W2121570734 @default.
• W4303044070 cites W2125955138 @default.
• W4303044070 cites W2132432514 @default.
• W4303044070 cites W2134028524 @default.
• W4303044070 cites W2139394837 @default.
• W4303044070 cites W2145502099 @default.
• W4303044070 cites W2146981677 @default.
• W4303044070 cites W2169456326 @default.
• W4303044070 cites W2173216716 @default.
• W4303044070 cites W2184159865 @default.
• W4303044070 cites W2189532371 @default.
• W4303044070 cites W2230025115 @default.
• W4303044070 cites W2257857567 @default.
• W4303044070 cites W2531369023 @default.
• W4303044070 cites W2626340527 @default.
• W4303044070 cites W2731956441 @default.
• W4303044070 cites W2734786717 @default.
• W4303044070 cites W2736984476 @default.
• W4303044070 cites W2765755121 @default.
• W4303044070 cites W2778955302 @default.
• W4303044070 cites W2790330822 @default.
• W4303044070 cites W2790944211 @default.
• W4303044070 cites W2795860999 @default.
• W4303044070 cites W2796322027 @default.
• W4303044070 cites W2800818747 @default.
• W4303044070 cites W2800941085 @default.
• W4303044070 cites W2808159773 @default.
• W4303044070 cites W2884946385 @default.
• W4303044070 cites W2899760200 @default.
• W4303044070 cites W2904374442 @default.
• W4303044070 cites W2904986382 @default.
• W4303044070 cites W2922375418 @default.
• W4303044070 cites W2945507544 @default.
• W4303044070 cites W2948560706 @default.
• W4303044070 cites W2950581333 @default.
• W4303044070 cites W2951954681 @default.
• W4303044070 cites W2971721461 @default.
• W4303044070 cites W2991354527 @default.
• W4303044070 cites W3018141250 @default.
• W4303044070 cites W3035048302 @default.
• W4303044070 cites W3037237874 @default.
• W4303044070 cites W3042423920 @default.
• W4303044070 cites W3096621422 @default.
• W4303044070 cites W3128016704 @default.
• W4303044070 cites W3140038269 @default.
• W4303044070 cites W3173413860 @default.
• W4303044070 cites W3198954077 @default.
• W4303044070 cites W3210879889 @default.

• next