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• W4303510715 startingPage "187" @default.
• W4303510715 abstract "Research using membrane capacitance (Cm) measurements in adrenal chromaffin cells has transformed our understanding of the molecular mechanisms controlling regulated exocytosis. This is in part due to the exquisite temporal resolution of the technique, and the possibility of combining quantification of exo-/endocytosis at the whole-cell level, with the ability to simultaneously monitor and control the calcium signals triggering vesicle fusion. In this regard, experiments performed with Cm measurements complement amperometry experiments that give a measure of secreted transmitter and the behavior of the fusion pore, and fluorescent microscopy studies used to monitor vesicle and protein dynamics in imaged regions of the cell. In this chapter, we provide a detailed account of the methodology used to perform whole-cell patch clamp measurements of Cm in combination with voltage-clamp recordings of voltage-gated calcium channels to quantify stimulus-secretion coupling in chromaffin cells. Stimulus protocols developed for investigation of functionally distinct releasable vesicle pools are also described." @default.
• W4303510715 created "2022-10-08" @default.
• W4303510715 creator A5052931275 @default.
• W4303510715 creator A5084558080 @default.
• W4303510715 date "2022-10-08" @default.
• W4303510715 modified "2023-10-14" @default.
• W4303510715 title "Membrane Capacitance Measurements of Stimulus-Evoked Exocytosis in Adrenal Chromaffin Cells" @default.
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• W4303510715 doi "https://doi.org/10.1007/978-1-0716-2671-9_13" @default.
• W4303510715 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36205895" @default.
• W4303510715 hasPublicationYear "2022" @default.
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