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- W4303520514 abstract "Abstract In tissue engineering, cells are grown often on scaffolds and subjected to chemical/mechanical stimuli. Most such cultures still use fetal bovine serum (FBS) despite its known disadvantages including ethical concerns, safety issues, and variability in composition, which greatly influences the experimental outcomes. To overcome the disadvantages of using FBS, chemically defined serum substitute medium needs to be developed. Development of such medium depends on cell type and application - which makes it impossible to define one universal serum substitute medium for all cells in any application. Here, we developed a serum substitute medium for bone tissue engineering (BTE) in a step-by-step process. Essential components were added to the medium while human bone marrow mesenchymal stromal cells (hBMSCs, osteoblast progenitor cells) were cultured in two-dimensional (2D) and three-dimensional (3D) substrates. In a 3-week culture, the developed serum substitute medium worked equally well as FBS containing medium in term of cell attachment to the substrate, cell survival, osteoblast differentiation, and deposition of extracellular matrix. In the next step, the use of serum substitute medium was evaluated when culturing cells under mechanical loading in the form of shear stress. The outcomes showed that the application of shear stress is essential to improve extracellular matrix formation while using serum substitute medium. The developed serum substitute medium could pave the way in replacing FBS for BTE studies eliminating the use of controversial FBS and providing a better-defined chemical environment for BTE studies. Graphical abstract" @default.
- W4303520514 created "2022-10-08" @default.
- W4303520514 creator A5001793836 @default.
- W4303520514 creator A5085927001 @default.
- W4303520514 creator A5091827843 @default.
- W4303520514 date "2022-10-07" @default.
- W4303520514 modified "2023-10-16" @default.
- W4303520514 title "Development of serum substitute medium for bone tissue engineering" @default.
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- W4303520514 cites W1965505631 @default.
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- W4303520514 cites W1969583812 @default.
- W4303520514 cites W1969779592 @default.
- W4303520514 cites W1973231843 @default.
- W4303520514 cites W1973461515 @default.
- W4303520514 cites W1976065936 @default.
- W4303520514 cites W1976675965 @default.
- W4303520514 cites W1977260464 @default.
- W4303520514 cites W1982912398 @default.
- W4303520514 cites W1990294254 @default.
- W4303520514 cites W2005098603 @default.
- W4303520514 cites W2007307882 @default.
- W4303520514 cites W2014397497 @default.
- W4303520514 cites W2018210705 @default.
- W4303520514 cites W2020075958 @default.
- W4303520514 cites W2020600683 @default.
- W4303520514 cites W2024533880 @default.
- W4303520514 cites W2027914516 @default.
- W4303520514 cites W2029898022 @default.
- W4303520514 cites W2030405870 @default.
- W4303520514 cites W2032051482 @default.
- W4303520514 cites W2032360452 @default.
- W4303520514 cites W2041178809 @default.
- W4303520514 cites W2041307278 @default.
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- W4303520514 cites W2122571576 @default.
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- W4303520514 cites W2153186849 @default.
- W4303520514 cites W2157214912 @default.
- W4303520514 cites W2166815593 @default.
- W4303520514 cites W2182832651 @default.
- W4303520514 cites W2289545277 @default.
- W4303520514 cites W2467765583 @default.
- W4303520514 cites W2521745780 @default.
- W4303520514 cites W2524169823 @default.
- W4303520514 cites W2556861557 @default.
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- W4303520514 doi "https://doi.org/10.1101/2022.10.07.511271" @default.
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