FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4303579222> ?p ?o ?g. }

• W4303579222 endingPage "2636" @default.
• W4303579222 startingPage "2636" @default.
• W4303579222 abstract "The technology of successful cryopreservation is a very important factor in research and commercial applications. However, the survival and development of the vitrified-thawed (VT) oocytes are lower than those of non-vitrified-thawed (non-VT) oocytes. This study investigated the effect of the addition of hydroxypropyl cellulose (HPC) to a vitrification solution of bovine oocytes. For the vitrification, bovine metaphase II oocytes were pretreated with a solution containing 10% ethylene glycol supplemented with 0, 10, 50, or 100 µg/mL HPC for 5 min, then exposed to a solution containing 30% ethylene glycol supplemented with 0, 10, 50, or 100 µg/mL HPC for 30 sec, and then directly plunged into liquid nitrogen. Oocytes exposed to 0, 10, 50, and 100 µg/mL HPC were named the 0, 10, 50, and 100 HPC groups, respectively. Samples were thawed via sequential incubation in Dulbecco’s phosphate-buffered saline (D-BPS) supplemented with 10% fetal bovine serum and decreasing concentrations of sucrose (1, 0.5, 0.25, and 0.125 M) for 1 min each time. After thawing, VT oocytes were treated at 0.05% hyaluronidase, and cumulus cells were removed by mechanical pipetting. The oocytes were washed with HEPES-buffered Tyrode’s medium and incubated in a droplet of previously cultured in vitro maturation medium for 1 h to recover. The survival rate of the oocytes was significantly higher in the 50 HPC group (84.2%) than in the 0 (75.4%), 10 (80.4%), and 100 (75.5%) HPC groups. The reactive oxygen species (ROS) levels of the non-VT and 50 HPC groups were lower than the 0, 10, and 100 HPC groups. The mRNA levels of proapoptotic genes (Bax) were lower in the non-VT, 0, and 50 HPC groups than in the other groups. The mRNA expression levels of antiapoptotic genes (BCl2) was higher in the non-VT than in the other groups. The mRNA level of a stress-related gene (Hsp70) was lower in the 50 HPC than in the other groups. At day 8, the developmental capacity of embryos obtained via parthenogenetic activation (PA) was determined in the non-VT, 0 HPC, and 50 HPC groups. The cleavage rate of the non-VT group was significantly higher, but the blastocyst development rate and total cell number per blastocyst did not significantly differ between the non-VT and 50 HPC groups. The mRNA levels of proapoptotic genes (Bax and Caspase-3) and a stress-related gene (Hsp70) were higher in the 0 HPC group than in the non-VT and 50 HPC groups. In conclusion, supplementation of vitrification solution with HPC improves the survival rate of VT bovine oocytes and the development capacity of embryos derived from these oocytes via PA." @default.
• W4303579222 created "2022-10-08" @default.
• W4303579222 creator A5006763502 @default.
• W4303579222 creator A5035117454 @default.
• W4303579222 creator A5043595101 @default.
• W4303579222 creator A5061846769 @default.
• W4303579222 creator A5063204521 @default.
• W4303579222 creator A5072769534 @default.
• W4303579222 creator A5079144393 @default.
• W4303579222 creator A5082276294 @default.
• W4303579222 creator A5085928279 @default.
• W4303579222 creator A5090393525 @default.
• W4303579222 date "2022-09-30" @default.
• W4303579222 modified "2023-09-26" @default.
• W4303579222 title "Effect of Supplementation of Cryoprotectant Solution with Hydroxypropyl Cellulose for Vitrification of Bovine Oocytes" @default.
• W4303579222 cites W1674405224 @default.
• W4303579222 cites W1795765514 @default.
• W4303579222 cites W1977114647 @default.
• W4303579222 cites W1977258127 @default.
• W4303579222 cites W1991209624 @default.
• W4303579222 cites W2000605323 @default.
• W4303579222 cites W2000758491 @default.
• W4303579222 cites W2017798219 @default.
• W4303579222 cites W2018304597 @default.
• W4303579222 cites W2022114917 @default.
• W4303579222 cites W2068812485 @default.
• W4303579222 cites W2079238504 @default.
• W4303579222 cites W2084535889 @default.
• W4303579222 cites W2085862980 @default.
• W4303579222 cites W2092142646 @default.
• W4303579222 cites W2102592982 @default.
• W4303579222 cites W2104256886 @default.
• W4303579222 cites W2107277218 @default.
• W4303579222 cites W2109474718 @default.
• W4303579222 cites W2116077034 @default.
• W4303579222 cites W2128571608 @default.
• W4303579222 cites W2158518415 @default.
• W4303579222 cites W2160030059 @default.
• W4303579222 cites W2161464304 @default.
• W4303579222 cites W2192080449 @default.
• W4303579222 cites W2236834725 @default.
• W4303579222 cites W2285706540 @default.
• W4303579222 cites W2292991999 @default.
• W4303579222 cites W2563237941 @default.
• W4303579222 cites W2566896798 @default.
• W4303579222 cites W2782674782 @default.
• W4303579222 cites W3049382689 @default.
• W4303579222 cites W62545516 @default.
• W4303579222 cites W821896841 @default.
• W4303579222 doi "https://doi.org/10.3390/ani12192636" @default.
• W4303579222 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36230376" @default.
• W4303579222 hasPublicationYear "2022" @default.
• W4303579222 type Work @default.
• W4303579222 citedByCount "0" @default.
• W4303579222 crossrefType "journal-article" @default.
• W4303579222 hasAuthorship W4303579222A5006763502 @default.
• W4303579222 hasAuthorship W4303579222A5035117454 @default.
• W4303579222 hasAuthorship W4303579222A5043595101 @default.
• W4303579222 hasAuthorship W4303579222A5061846769 @default.
• W4303579222 hasAuthorship W4303579222A5063204521 @default.
• W4303579222 hasAuthorship W4303579222A5072769534 @default.
• W4303579222 hasAuthorship W4303579222A5079144393 @default.
• W4303579222 hasAuthorship W4303579222A5082276294 @default.
• W4303579222 hasAuthorship W4303579222A5085928279 @default.
• W4303579222 hasAuthorship W4303579222A5090393525 @default.
• W4303579222 hasBestOaLocation W43035792221 @default.
• W4303579222 hasConcept C13965031 @default.
• W4303579222 hasConcept C16685009 @default.
• W4303579222 hasConcept C178790620 @default.
• W4303579222 hasConcept C179933525 @default.
• W4303579222 hasConcept C185592680 @default.
• W4303579222 hasConcept C196843134 @default.
• W4303579222 hasConcept C202751555 @default.
• W4303579222 hasConcept C25642318 @default.
• W4303579222 hasConcept C2777516009 @default.
• W4303579222 hasConcept C2778022349 @default.
• W4303579222 hasConcept C2779404806 @default.
• W4303579222 hasConcept C55493867 @default.
• W4303579222 hasConcept C68528507 @default.
• W4303579222 hasConcept C71924100 @default.
• W4303579222 hasConcept C86803240 @default.
• W4303579222 hasConcept C95444343 @default.
• W4303579222 hasConceptScore W4303579222C13965031 @default.
• W4303579222 hasConceptScore W4303579222C16685009 @default.
• W4303579222 hasConceptScore W4303579222C178790620 @default.
• W4303579222 hasConceptScore W4303579222C179933525 @default.
• W4303579222 hasConceptScore W4303579222C185592680 @default.
• W4303579222 hasConceptScore W4303579222C196843134 @default.
• W4303579222 hasConceptScore W4303579222C202751555 @default.
• W4303579222 hasConceptScore W4303579222C25642318 @default.
• W4303579222 hasConceptScore W4303579222C2777516009 @default.
• W4303579222 hasConceptScore W4303579222C2778022349 @default.
• W4303579222 hasConceptScore W4303579222C2779404806 @default.
• W4303579222 hasConceptScore W4303579222C55493867 @default.
• W4303579222 hasConceptScore W4303579222C68528507 @default.
• W4303579222 hasConceptScore W4303579222C71924100 @default.
• W4303579222 hasConceptScore W4303579222C86803240 @default.
• W4303579222 hasConceptScore W4303579222C95444343 @default.

• next