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- W4303699288 endingPage "11831" @default.
- W4303699288 startingPage "11831" @default.
- W4303699288 abstract "Cytolethal distending toxins (Cdt) are produced by a diverse group of pathogens. One Cdt-producing organism, Aggregatibacter actinomycetemcomitans, plays a critical role in the pathogenesis of a unique form of periodontitis, formerly referred to as localized aggressive periodontitis. The active Cdt subunit, CdtB, is a potent phosphatidylinositol (PI) 3,4,5-triphosphate phosphatase capable of inducing PI-3-kinase signaling blockade, a requisite for Cdt-induced toxicity in lymphocytes. In this study, we extended our observations to include the oral keratinocyte response to AaCdt using cell lines and primary gingival keratinocytes. All three exhibited G2/M arrest when exposed to AaCdt toxin within 24 h. Toxin-treated cells exhibited reduced levels of pAkt and pGSK3β within 6 h. Pre-treatment with GSK3β kinase inhibitors, LY2090314, CHIR99021 and Tideglusib, abrogated Cdt-induced G2/M arrest. None of the oral epithelial cells exhibited evidence of apoptosis. Cells remained arrested in the G2/M phase for at least 72 h without evidence of DNA damage response activation (H2AX phosphorylation). Cdt-treated cells displayed increased phosphorylation of the cyclin dependent kinase 1 (CDK1); moreover, the GSK3 inhibitors blocked this increase and reduced total CDK1 levels. This study further clarifies the potential mechanism(s) contributing to Cdt toxicity and toxin-mediated pathogenesis." @default.
- W4303699288 created "2022-10-09" @default.
- W4303699288 creator A5030654407 @default.
- W4303699288 creator A5046240883 @default.
- W4303699288 creator A5053742796 @default.
- W4303699288 creator A5087731817 @default.
- W4303699288 creator A5090087970 @default.
- W4303699288 date "2022-10-05" @default.
- W4303699288 modified "2023-10-09" @default.
- W4303699288 title "Aggregatibacter actinomycetemcomitans Cytolethal Distending Toxin-Induces Cell Cycle Arrest in a Glycogen Synthase Kinase (GSK)-3-Dependent Manner in Oral Keratinocytes" @default.
- W4303699288 cites W1491715499 @default.
- W4303699288 cites W1502036442 @default.
- W4303699288 cites W1514344797 @default.
- W4303699288 cites W1530842205 @default.
- W4303699288 cites W1537669128 @default.
- W4303699288 cites W1539361639 @default.
- W4303699288 cites W1584744610 @default.
- W4303699288 cites W1647737309 @default.
- W4303699288 cites W1681283868 @default.
- W4303699288 cites W1745772131 @default.
- W4303699288 cites W1870774590 @default.
- W4303699288 cites W1947232614 @default.
- W4303699288 cites W1966462033 @default.
- W4303699288 cites W1971732228 @default.
- W4303699288 cites W1982370572 @default.
- W4303699288 cites W1988587367 @default.
- W4303699288 cites W1989930451 @default.
- W4303699288 cites W1999881785 @default.
- W4303699288 cites W2004532530 @default.
- W4303699288 cites W2005546292 @default.
- W4303699288 cites W2010179265 @default.
- W4303699288 cites W2010372854 @default.
- W4303699288 cites W2011546738 @default.
- W4303699288 cites W2017039914 @default.
- W4303699288 cites W2017615730 @default.
- W4303699288 cites W2018280617 @default.
- W4303699288 cites W2020598630 @default.
- W4303699288 cites W2023663419 @default.
- W4303699288 cites W2024716660 @default.
- W4303699288 cites W2027551392 @default.
- W4303699288 cites W2030553516 @default.
- W4303699288 cites W2046240664 @default.
- W4303699288 cites W2048499353 @default.
- W4303699288 cites W2050515087 @default.
- W4303699288 cites W2056909568 @default.
- W4303699288 cites W2057785174 @default.
- W4303699288 cites W2058936887 @default.
- W4303699288 cites W2060159583 @default.
- W4303699288 cites W2060510631 @default.
- W4303699288 cites W2089466992 @default.
- W4303699288 cites W2107177655 @default.
- W4303699288 cites W2109571718 @default.
- W4303699288 cites W2111606297 @default.
- W4303699288 cites W2112176493 @default.
- W4303699288 cites W2116652997 @default.
- W4303699288 cites W2116892114 @default.
- W4303699288 cites W2122679010 @default.
- W4303699288 cites W2124127848 @default.
- W4303699288 cites W2125169413 @default.
- W4303699288 cites W2129214738 @default.
- W4303699288 cites W2129761951 @default.
- W4303699288 cites W2130092897 @default.
- W4303699288 cites W2133123988 @default.
- W4303699288 cites W2135906691 @default.
- W4303699288 cites W2136240342 @default.
- W4303699288 cites W2138296062 @default.
- W4303699288 cites W2139175224 @default.
- W4303699288 cites W2140748993 @default.
- W4303699288 cites W2147474032 @default.
- W4303699288 cites W2148524979 @default.
- W4303699288 cites W2149287828 @default.
- W4303699288 cites W2152639662 @default.
- W4303699288 cites W2159363600 @default.
- W4303699288 cites W2159570897 @default.
- W4303699288 cites W2165131836 @default.
- W4303699288 cites W2167345732 @default.
- W4303699288 cites W2256600804 @default.
- W4303699288 cites W2319332114 @default.
- W4303699288 cites W2347135114 @default.
- W4303699288 cites W2415112986 @default.
- W4303699288 cites W2511048520 @default.
- W4303699288 cites W2532933930 @default.
- W4303699288 cites W2558584423 @default.
- W4303699288 cites W2581326404 @default.
- W4303699288 cites W2618354387 @default.
- W4303699288 cites W2733561513 @default.
- W4303699288 cites W2771165587 @default.
- W4303699288 cites W2891394363 @default.
- W4303699288 cites W2936471677 @default.
- W4303699288 cites W2963462627 @default.
- W4303699288 cites W2981008959 @default.
- W4303699288 cites W2997002901 @default.
- W4303699288 cites W3008092645 @default.
- W4303699288 cites W3008402211 @default.
- W4303699288 cites W3032979845 @default.
- W4303699288 cites W3035065189 @default.
- W4303699288 cites W3134907435 @default.
- W4303699288 cites W3150970321 @default.