Matches in SemOpenAlex for { <https://semopenalex.org/work/W4303946589> ?p ?o ?g. }
- W4303946589 endingPage "3164" @default.
- W4303946589 startingPage "3164" @default.
- W4303946589 abstract "The functions of human testicular peritubular cells (HTPCs), forming a small compartment located between the seminiferous epithelium and the interstitial areas of the testis, are not fully known but go beyond intratesticular sperm transport and include immunological roles. The expression of the glucocorticoid receptor (GR) indicates that they may be regulated by glucocorticoids (GCs). Herein, we studied the consequences of the GC dexamethasone (Dex) in cultured HTPCs, which serves as a unique window into the human testis. We examined changes in cytokines, mainly by qPCR and ELISA. A holistic mass-spectrometry-based proteome analysis of cellular and secreted proteins was also performed. Dex, used in a therapeutic concentration, decreased the transcript level of proinflammatory cytokines, e.g., IL6, IL8 and MCP1. An siRNA-mediated knockdown of GR reduced the actions on IL6. Changes in IL6 were confirmed by ELISA measurements. Of note, Dex also lowered GR levels. The proteomic results revealed strong responses after 24 h (31 significantly altered cellular proteins) and more pronounced ones after 72 h of Dex exposure (30 less abundant and 42 more abundant cellular proteins). Dex also altered the composition of the secretome (33 proteins decreased, 13 increased) after 72 h. Among the regulated proteins were extracellular matrix (ECM) and basement membrane components (e.g., FBLN2, COL1A2 and COL3A1), as well as PTX3 and StAR. These results pinpoint novel, profound effects of Dex in HTPCs. If transferrable to the human testis, changes specifically in ECM and the immunological state of the testis may occur in men upon treatment with Dex for medical reasons." @default.
- W4303946589 created "2022-10-10" @default.
- W4303946589 creator A5006307023 @default.
- W4303946589 creator A5010867829 @default.
- W4303946589 creator A5012993117 @default.
- W4303946589 creator A5015131489 @default.
- W4303946589 creator A5017758310 @default.
- W4303946589 creator A5040291532 @default.
- W4303946589 creator A5049468599 @default.
- W4303946589 creator A5049612036 @default.
- W4303946589 creator A5052790584 @default.
- W4303946589 creator A5060411385 @default.
- W4303946589 creator A5070758515 @default.
- W4303946589 creator A5087795730 @default.
- W4303946589 date "2022-10-09" @default.
- W4303946589 modified "2023-09-27" @default.
- W4303946589 title "Profound Effects of Dexamethasone on the Immunological State, Synthesis and Secretion Capacity of Human Testicular Peritubular Cells" @default.
- W4303946589 cites W1497186327 @default.
- W4303946589 cites W1541382336 @default.
- W4303946589 cites W1965436839 @default.
- W4303946589 cites W1970703629 @default.
- W4303946589 cites W1971639265 @default.
- W4303946589 cites W1977549713 @default.
- W4303946589 cites W1978800544 @default.
- W4303946589 cites W1982688882 @default.
- W4303946589 cites W1985374659 @default.
- W4303946589 cites W1987960919 @default.
- W4303946589 cites W1991755452 @default.
- W4303946589 cites W1993744211 @default.
- W4303946589 cites W1995116585 @default.
- W4303946589 cites W1996462110 @default.
- W4303946589 cites W1998086294 @default.
- W4303946589 cites W2004542682 @default.
- W4303946589 cites W2005439798 @default.
- W4303946589 cites W2010266016 @default.
- W4303946589 cites W2013269855 @default.
- W4303946589 cites W2018003897 @default.
- W4303946589 cites W2022742958 @default.
- W4303946589 cites W2024841481 @default.
- W4303946589 cites W2033274468 @default.
- W4303946589 cites W2041884756 @default.
- W4303946589 cites W2045045884 @default.
- W4303946589 cites W2061700679 @default.
- W4303946589 cites W2062461025 @default.
- W4303946589 cites W2063340850 @default.
- W4303946589 cites W2063394409 @default.
- W4303946589 cites W2063742006 @default.
- W4303946589 cites W2069288651 @default.
- W4303946589 cites W2072674959 @default.
- W4303946589 cites W2077550626 @default.
- W4303946589 cites W2079533675 @default.
- W4303946589 cites W2081373660 @default.
- W4303946589 cites W2085628651 @default.
- W4303946589 cites W2097093976 @default.
- W4303946589 cites W2105563927 @default.
- W4303946589 cites W2108244474 @default.
- W4303946589 cites W2111674272 @default.
- W4303946589 cites W2119284420 @default.
- W4303946589 cites W2122341014 @default.
- W4303946589 cites W2128330079 @default.
- W4303946589 cites W2130132367 @default.
- W4303946589 cites W2139674110 @default.
- W4303946589 cites W2142663838 @default.
- W4303946589 cites W2143208648 @default.
- W4303946589 cites W2146864292 @default.
- W4303946589 cites W2148362913 @default.
- W4303946589 cites W2152833835 @default.
- W4303946589 cites W2158021558 @default.
- W4303946589 cites W2158217645 @default.
- W4303946589 cites W2163820623 @default.
- W4303946589 cites W2165412300 @default.
- W4303946589 cites W2168074819 @default.
- W4303946589 cites W2169090691 @default.
- W4303946589 cites W2170726898 @default.
- W4303946589 cites W2191883975 @default.
- W4303946589 cites W2193183716 @default.
- W4303946589 cites W2412545608 @default.
- W4303946589 cites W2412814116 @default.
- W4303946589 cites W2414003634 @default.
- W4303946589 cites W2544360569 @default.
- W4303946589 cites W2556591952 @default.
- W4303946589 cites W256764205 @default.
- W4303946589 cites W2583971061 @default.
- W4303946589 cites W2613553574 @default.
- W4303946589 cites W2761424079 @default.
- W4303946589 cites W2781657596 @default.
- W4303946589 cites W2793133292 @default.
- W4303946589 cites W2805740127 @default.
- W4303946589 cites W2807526022 @default.
- W4303946589 cites W2884060324 @default.
- W4303946589 cites W2888483344 @default.
- W4303946589 cites W2899760200 @default.
- W4303946589 cites W2914650668 @default.
- W4303946589 cites W2915331440 @default.
- W4303946589 cites W2926015082 @default.
- W4303946589 cites W2956004222 @default.
- W4303946589 cites W2957947066 @default.
- W4303946589 cites W2971248963 @default.