Matches in SemOpenAlex for { <https://semopenalex.org/work/W4304014013> ?p ?o ?g. }
- W4304014013 endingPage "3900.e10" @default.
- W4304014013 startingPage "3885" @default.
- W4304014013 abstract "RNA can regulate its own synthesis without auxiliary proteins. For example, U-rich RNA sequences signal RNA polymerase (RNAP) to pause transcription and are required for transcript release at intrinsic terminators in all kingdoms of life. In contrast, the regulatory RNA putL suppresses pausing and termination in cis. However, how nascent RNA modulates its own synthesis remains largely unknown. We present cryo-EM reconstructions of RNAP captured during transcription of putL variants or an unrelated sequence at a U-rich pause site. Our results suggest how putL suppresses pausing and promotes its synthesis. We demonstrate that transcribing a U-rich sequence, a ubiquitous trigger of intrinsic termination, promotes widening of the RNAP nucleic-acid-binding channel. Widening destabilizes RNAP interactions with DNA and RNA to facilitate transcript dissociation reminiscent of intrinsic transcription termination. Surprisingly, RNAP remains bound to DNA after transcript release. Our results provide the structural framework to understand RNA-mediated intrinsic transcription termination." @default.
- W4304014013 created "2022-10-10" @default.
- W4304014013 creator A5017752225 @default.
- W4304014013 creator A5020496528 @default.
- W4304014013 creator A5024432479 @default.
- W4304014013 creator A5044647144 @default.
- W4304014013 creator A5057662264 @default.
- W4304014013 creator A5070966017 @default.
- W4304014013 creator A5072274843 @default.
- W4304014013 date "2022-10-01" @default.
- W4304014013 modified "2023-10-15" @default.
- W4304014013 title "Structural insights into RNA-mediated transcription regulation in bacteria" @default.
- W4304014013 cites W1568431853 @default.
- W4304014013 cites W1593439623 @default.
- W4304014013 cites W1605780295 @default.
- W4304014013 cites W1894497412 @default.
- W4304014013 cites W1964836599 @default.
- W4304014013 cites W1968821031 @default.
- W4304014013 cites W1978139942 @default.
- W4304014013 cites W1987568156 @default.
- W4304014013 cites W1989380134 @default.
- W4304014013 cites W1989736935 @default.
- W4304014013 cites W1999407496 @default.
- W4304014013 cites W1999434982 @default.
- W4304014013 cites W2000986651 @default.
- W4304014013 cites W2012019201 @default.
- W4304014013 cites W2014839221 @default.
- W4304014013 cites W2018166918 @default.
- W4304014013 cites W2020055579 @default.
- W4304014013 cites W2024651141 @default.
- W4304014013 cites W2027131183 @default.
- W4304014013 cites W2031040926 @default.
- W4304014013 cites W2034311984 @default.
- W4304014013 cites W2034319418 @default.
- W4304014013 cites W2034421156 @default.
- W4304014013 cites W2055149800 @default.
- W4304014013 cites W2056575553 @default.
- W4304014013 cites W2057276918 @default.
- W4304014013 cites W2067258168 @default.
- W4304014013 cites W2068914602 @default.
- W4304014013 cites W2074206760 @default.
- W4304014013 cites W2074416623 @default.
- W4304014013 cites W2077064224 @default.
- W4304014013 cites W2078290510 @default.
- W4304014013 cites W2079337189 @default.
- W4304014013 cites W2085067952 @default.
- W4304014013 cites W2086561953 @default.
- W4304014013 cites W2094671300 @default.
- W4304014013 cites W2102284571 @default.
- W4304014013 cites W2107870109 @default.
- W4304014013 cites W2108921801 @default.
- W4304014013 cites W2124026197 @default.
- W4304014013 cites W2126529674 @default.
- W4304014013 cites W2128104161 @default.
- W4304014013 cites W2129315441 @default.
- W4304014013 cites W2132629607 @default.
- W4304014013 cites W2132920850 @default.
- W4304014013 cites W2134952259 @default.
- W4304014013 cites W2135296886 @default.
- W4304014013 cites W2140803417 @default.
- W4304014013 cites W2144902201 @default.
- W4304014013 cites W2145530963 @default.
- W4304014013 cites W2160466762 @default.
- W4304014013 cites W2161424731 @default.
- W4304014013 cites W2224531996 @default.
- W4304014013 cites W2522887125 @default.
- W4304014013 cites W2587625522 @default.
- W4304014013 cites W2593511418 @default.
- W4304014013 cites W2599962490 @default.
- W4304014013 cites W2620712420 @default.
- W4304014013 cites W2697942002 @default.
- W4304014013 cites W2760056442 @default.
- W4304014013 cites W2790099298 @default.
- W4304014013 cites W2791783580 @default.
- W4304014013 cites W2793611243 @default.
- W4304014013 cites W2797492921 @default.
- W4304014013 cites W2801376357 @default.
- W4304014013 cites W2804014665 @default.
- W4304014013 cites W2899714098 @default.
- W4304014013 cites W2916993760 @default.
- W4304014013 cites W2946394399 @default.
- W4304014013 cites W2951489799 @default.
- W4304014013 cites W2953320394 @default.
- W4304014013 cites W2971173928 @default.
- W4304014013 cites W2973457155 @default.
- W4304014013 cites W3000981556 @default.
- W4304014013 cites W3001359786 @default.
- W4304014013 cites W3080219868 @default.
- W4304014013 cites W3083406432 @default.
- W4304014013 cites W3108663073 @default.
- W4304014013 cites W3114119493 @default.
- W4304014013 cites W3131760520 @default.
- W4304014013 cites W3182767159 @default.
- W4304014013 cites W3206875029 @default.
- W4304014013 cites W4220764899 @default.
- W4304014013 cites W4291551976 @default.
- W4304014013 doi "https://doi.org/10.1016/j.molcel.2022.09.020" @default.
- W4304014013 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36220101" @default.