Matches in SemOpenAlex for { <https://semopenalex.org/work/W4304084269> ?p ?o ?g. }
- W4304084269 endingPage "1402" @default.
- W4304084269 startingPage "1393" @default.
- W4304084269 abstract "Abstract We present Light-Seq, an approach for multiplexed spatial indexing of intact biological samples using light-directed DNA barcoding in fixed cells and tissues followed by ex situ sequencing. Light-Seq combines spatially targeted, rapid photocrosslinking of DNA barcodes onto complementary DNAs in situ with a one-step DNA stitching reaction to create pooled, spatially indexed sequencing libraries. This light-directed barcoding enables in situ selection of multiple cell populations in intact fixed tissue samples for full-transcriptome sequencing based on location, morphology or protein stains, without cellular dissociation. Applying Light-Seq to mouse retinal sections, we recovered thousands of differentially enriched transcripts from three cellular layers and discovered biomarkers for a very rare neuronal subtype, dopaminergic amacrine cells, from only four to eight individual cells per section. Light-Seq provides an accessible workflow to combine in situ imaging and protein staining with next generation sequencing of the same cells, leaving the sample intact for further analysis post-sequencing." @default.
- W4304084269 created "2022-10-10" @default.
- W4304084269 creator A5009896683 @default.
- W4304084269 creator A5019110059 @default.
- W4304084269 creator A5031583346 @default.
- W4304084269 creator A5032594307 @default.
- W4304084269 creator A5045640072 @default.
- W4304084269 creator A5059621249 @default.
- W4304084269 creator A5067094283 @default.
- W4304084269 creator A5079216599 @default.
- W4304084269 creator A5088190941 @default.
- W4304084269 date "2022-10-10" @default.
- W4304084269 modified "2023-10-18" @default.
- W4304084269 title "Light-Seq: light-directed in situ barcoding of biomolecules in fixed cells and tissues for spatially indexed sequencing" @default.
- W4304084269 cites W1540870789 @default.
- W4304084269 cites W1542309476 @default.
- W4304084269 cites W1617520624 @default.
- W4304084269 cites W1893444436 @default.
- W4304084269 cites W1968068562 @default.
- W4304084269 cites W1972618510 @default.
- W4304084269 cites W1975395953 @default.
- W4304084269 cites W1989020100 @default.
- W4304084269 cites W1990880789 @default.
- W4304084269 cites W1996813404 @default.
- W4304084269 cites W1998757683 @default.
- W4304084269 cites W2017461303 @default.
- W4304084269 cites W2027085605 @default.
- W4304084269 cites W2055043387 @default.
- W4304084269 cites W2065635189 @default.
- W4304084269 cites W2066852475 @default.
- W4304084269 cites W2077830471 @default.
- W4304084269 cites W2092977521 @default.
- W4304084269 cites W2105071916 @default.
- W4304084269 cites W2108813964 @default.
- W4304084269 cites W2117131162 @default.
- W4304084269 cites W2119527481 @default.
- W4304084269 cites W2121466988 @default.
- W4304084269 cites W2136145671 @default.
- W4304084269 cites W2137586531 @default.
- W4304084269 cites W2138207763 @default.
- W4304084269 cites W2139357174 @default.
- W4304084269 cites W2146843966 @default.
- W4304084269 cites W2151013106 @default.
- W4304084269 cites W2152532695 @default.
- W4304084269 cites W2154574215 @default.
- W4304084269 cites W2157139472 @default.
- W4304084269 cites W2167279371 @default.
- W4304084269 cites W2169456326 @default.
- W4304084269 cites W2179438025 @default.
- W4304084269 cites W2325822724 @default.
- W4304084269 cites W2471536144 @default.
- W4304084269 cites W2510746232 @default.
- W4304084269 cites W2520858613 @default.
- W4304084269 cites W2579733900 @default.
- W4304084269 cites W2626166598 @default.
- W4304084269 cites W2767343818 @default.
- W4304084269 cites W2771767991 @default.
- W4304084269 cites W2832881433 @default.
- W4304084269 cites W2888971284 @default.
- W4304084269 cites W2915848182 @default.
- W4304084269 cites W2923300855 @default.
- W4304084269 cites W2945773152 @default.
- W4304084269 cites W2951268836 @default.
- W4304084269 cites W2952109315 @default.
- W4304084269 cites W2952362302 @default.
- W4304084269 cites W2953177586 @default.
- W4304084269 cites W2965275351 @default.
- W4304084269 cites W2968292465 @default.
- W4304084269 cites W2969558305 @default.
- W4304084269 cites W2972983657 @default.
- W4304084269 cites W2974555969 @default.
- W4304084269 cites W2981727226 @default.
- W4304084269 cites W2996577138 @default.
- W4304084269 cites W3012809091 @default.
- W4304084269 cites W3021920958 @default.
- W4304084269 cites W3023701177 @default.
- W4304084269 cites W3029650759 @default.
- W4304084269 cites W3033098366 @default.
- W4304084269 cites W3039340968 @default.
- W4304084269 cites W3047081808 @default.
- W4304084269 cites W3088384177 @default.
- W4304084269 cites W3089334516 @default.
- W4304084269 cites W3090542854 @default.
- W4304084269 cites W3100967665 @default.
- W4304084269 cites W3112563877 @default.
- W4304084269 cites W3113630028 @default.
- W4304084269 cites W3114643239 @default.
- W4304084269 cites W3167327839 @default.
- W4304084269 cites W3176766013 @default.
- W4304084269 cites W3181969337 @default.
- W4304084269 cites W3184421840 @default.
- W4304084269 cites W3206454724 @default.
- W4304084269 cites W3211268553 @default.
- W4304084269 cites W4205812688 @default.
- W4304084269 cites W4206511670 @default.
- W4304084269 cites W4220765701 @default.
- W4304084269 cites W4220882167 @default.
- W4304084269 cites W4225563096 @default.