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- W43042885 abstract "Az RNS silencing, egy geninaktivacios mechanizmus, amely szinte az osszes eukariota szervezetben műkodik. Kutatasaink soran feltartuk a Cymbidium ringspot virus genomrol keződő small interferalo (si) RNS eredetet nagy hatekonysagu 454 (Life Science) es Soplexa (illumina) szekvenalo rendszerek alkalmazasaval. A virus genomrol szarmazo kis RNS-eket raterkepeztuk a virus genomjara, amely alapjan forro pontokat tudtunk azonositani. Igazoltuk,hogy virus siRNS-ek tulnyomo tobsege a virus pozitiv szalarol szarmazik, es 21-22 nukleotid (nt) hosszu. Megallapitottuk, hogy virus siRNS-ekkel toltott RISC (RNA Induced Silencing Complex) komplexek szekvenciaspecifikusan hasitjak a virus genomot. Szamos silencing szupresszor feherje (p19, HC-Pro, es p122) reszletes analizisevel igazoltuk, hogy a noveny antiviralis valaszat, a virus kodolta silencing szupresszorok hatekonyan gatoljak. Bizonyitottuk, hogy a siRNS-ek specifikus kotese es inaktivalasa a legelterjedtebb strategia a silencing szupresszor feherjek kozott. Feltartuk, hogy a silencing szuppresszor feherjek egy jelentős csoportja gatolja novenyek endogen siRNS es miRNS biogeneziset. A silencing szupressor feherjek interakcioja az endogen silencing utvonalakkal feltehetően a magyarazata a virus okozta tunetek kialakulasanak, hiszen a szupresszor feherjek sulyosan zavarja noveny egyedfejlődeset. | RNA silencing is a gene inactivation mechanism, which is conserved in a broad range of eukaryotes. The central players in RNA-mediated gene silencing are the small 21-24 nucleotide long RNA molecules engaged in sequence-specific interactions to inhibit gene expression. RNA silencing fulfils fundamental regulatory roles, as well as antiviral functions. We profiled viral siRNAs using two different high-throughput sequencing platforms. Both deep sequencing techniques revealed a strong bias in viral siRNAs for the positive strand of the virus and identified regions on the viral genome that produced viral siRNA in much higher abundance than other regions. We also analysed the viral RNA targeting by virus induced gene silencing in tombusvirus infected plants, and we show evidence that antiviral response is based on viral RNA cleavage by RNA-induced silencing effector complex (RISC) programmed by virus-specific siRNAs.. To counteract RNA silencing, viruses express silencing suppressors that interfere with both siRNA- and microRNA-guided silencing pathways. We used comparative approaches to analyse the molecular mechanism of suppression by three well-studied silencing suppressors. We found that silencing suppressors p19, p21 and HC-Pro each inhibit the RISC assembly. We demonstrated that these suppressors are able to interact with the endogenous silencing pathways suggesting that these interactions have an important role in the development of virus-induced symptoms." @default.
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- W43042885 date "2009-01-01" @default.
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- W43042885 title "Az RNS silencing szerepe, mechanizmusa a vírus gazda kölcsönhatásban = The role and the mechanism of RNA silencing in the plant virus interplay" @default.
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