FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4304606711> ?p ?o ?g. }

• W4304606711 abstract "Abstract Heme b (iron protoporphyrin IX) plays important roles in biology as a metallocofactor and signaling molecule. However, the targets of heme signaling and the network of proteins that mediate the exchange of heme from sites of synthesis or uptake to heme dependent or regulated proteins are poorly understood. Herein, we describe a quantitative mass spectrometry-based chemoproteomics strategy to identify exchange labile hemoproteins in human embryonic kidney HEK293 cells that may be relevant to heme signaling and trafficking. The strategy involves depleting endogenous heme with the heme biosynthetic inhibitor succinylacetone (SA), leaving putative heme binding proteins in their apo- state, followed by the capture of those proteins using hemin-agarose resin and finally elution and identification by mass spectrometry. By identifying only those proteins that interact with high specificity to hemin-agarose relative to control beaded agarose in a SA-dependent manner, we have expanded the number of proteins and ontologies that may be involved in binding and buffering labile heme or are targets of heme signaling. Notably, these include proteins involved in chromatin remodeling, DNA damage response, RNA splicing, cytoskeletal organization and vesicular trafficking, many of which have been associated with heme through complimentary studies published recently. Taken together, these results provide support for the emerging role for heme in an expanded set of cellular processes from genome integrity to protein trafficking and beyond." @default.
• W4304606711 created "2022-10-12" @default.
• W4304606711 creator A5013950313 @default.
• W4304606711 creator A5018713040 @default.
• W4304606711 creator A5023494464 @default.
• W4304606711 creator A5028128648 @default.
• W4304606711 creator A5036862812 @default.
• W4304606711 creator A5044996344 @default.
• W4304606711 creator A5073159230 @default.
• W4304606711 date "2022-10-11" @default.
• W4304606711 modified "2023-09-29" @default.
• W4304606711 title "Depletion Assisted Hemin Affinity (DAsHA) Proteomics Reveals an Expanded Landscape of Heme Binding Proteins" @default.
• W4304606711 cites W1572091120 @default.
• W4304606711 cites W1970286438 @default.
• W4304606711 cites W1974866485 @default.
• W4304606711 cites W1975191711 @default.
• W4304606711 cites W1982293081 @default.
• W4304606711 cites W1982855811 @default.
• W4304606711 cites W1983653026 @default.
• W4304606711 cites W1984887661 @default.
• W4304606711 cites W1989584376 @default.
• W4304606711 cites W1990174623 @default.
• W4304606711 cites W2003037278 @default.
• W4304606711 cites W2003765714 @default.
• W4304606711 cites W2006620950 @default.
• W4304606711 cites W2006636673 @default.
• W4304606711 cites W2006754144 @default.
• W4304606711 cites W2016317526 @default.
• W4304606711 cites W2025368509 @default.
• W4304606711 cites W2031624484 @default.
• W4304606711 cites W2034397996 @default.
• W4304606711 cites W2037402061 @default.
• W4304606711 cites W2046671797 @default.
• W4304606711 cites W2054303797 @default.
• W4304606711 cites W2054933597 @default.
• W4304606711 cites W2055751200 @default.
• W4304606711 cites W2057726198 @default.
• W4304606711 cites W2059441862 @default.
• W4304606711 cites W2070647295 @default.
• W4304606711 cites W2075572654 @default.
• W4304606711 cites W2076309563 @default.
• W4304606711 cites W2094190961 @default.
• W4304606711 cites W2098100414 @default.
• W4304606711 cites W2101821132 @default.
• W4304606711 cites W2105093529 @default.
• W4304606711 cites W2117960203 @default.
• W4304606711 cites W2126362614 @default.
• W4304606711 cites W2132492733 @default.
• W4304606711 cites W2139529165 @default.
• W4304606711 cites W2147219401 @default.
• W4304606711 cites W2154828593 @default.
• W4304606711 cites W2265806198 @default.
• W4304606711 cites W2296998275 @default.
• W4304606711 cites W2307314426 @default.
• W4304606711 cites W2317873538 @default.
• W4304606711 cites W2328981465 @default.
• W4304606711 cites W2401231048 @default.
• W4304606711 cites W2415954632 @default.
• W4304606711 cites W2416948272 @default.
• W4304606711 cites W2501641743 @default.
• W4304606711 cites W2507522383 @default.
• W4304606711 cites W2509947845 @default.
• W4304606711 cites W2549217003 @default.
• W4304606711 cites W2587901804 @default.
• W4304606711 cites W2593684489 @default.
• W4304606711 cites W2597299286 @default.
• W4304606711 cites W2747461361 @default.
• W4304606711 cites W2769158750 @default.
• W4304606711 cites W2784370033 @default.
• W4304606711 cites W2808885197 @default.
• W4304606711 cites W2883611877 @default.
• W4304606711 cites W2885206159 @default.
• W4304606711 cites W2887466728 @default.
• W4304606711 cites W2892171374 @default.
• W4304606711 cites W2896796940 @default.
• W4304606711 cites W2910201141 @default.
• W4304606711 cites W2959434609 @default.
• W4304606711 cites W2969996564 @default.
• W4304606711 cites W2981832578 @default.
• W4304606711 cites W2991327056 @default.
• W4304606711 cites W2999586868 @default.
• W4304606711 cites W3013142914 @default.
• W4304606711 cites W3014004550 @default.
• W4304606711 cites W3014576903 @default.
• W4304606711 cites W3020709906 @default.
• W4304606711 cites W3031744554 @default.
• W4304606711 cites W3033455113 @default.
• W4304606711 cites W3037186665 @default.
• W4304606711 cites W3039244969 @default.
• W4304606711 cites W3045618311 @default.
• W4304606711 cites W3049092629 @default.
• W4304606711 cites W3091438849 @default.
• W4304606711 cites W3094284950 @default.
• W4304606711 cites W3094622130 @default.
• W4304606711 cites W3112045069 @default.
• W4304606711 cites W3120495139 @default.
• W4304606711 cites W3165621829 @default.
• W4304606711 cites W3186901367 @default.
• W4304606711 cites W3191968980 @default.
• W4304606711 cites W3198361073 @default.

• next