FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4304687622> ?p ?o ?g. }

• W4304687622 endingPage "146923" @default.
• W4304687622 startingPage "146923" @default.
• W4304687622 abstract "Iron (Fe) is considered as an essential micronutrient due to its diverse functions in living systems. However, regulation of free iron levels is essential because free Fe ions, in excess, induce biological toxicity through different routes, including production of reactive oxygen species. Ferritin proteins play a vital role in controlling free Fe ion homeostasis by sequestering excess iron in the body. Ferritins comprise an H subunit with a ferroxidase center and an L subunit with a Fe nucleation site. However, lower vertebrates such as fish harbor an additional subunit termed ferritin M, which shows the characteristic features of both H and L. In this study, two ferritin subunits (H and M) with ferroxidase centers were identified and characterized from red-lip mullet (Liza haematocheila). The open reading frames of red-lip mullet ferritin H (LhFerH) and ferritin M−like (LhFerM) subunits comprise 534 and 531 bps, which encode for putative polypeptides of 177 and 176 amino acids, respectively. LhFerH and LhFerM were found to retain well-conserved residues, including seven ferroxidase di-iron centers, characteristic domains, and signatures of their known homologs. We cloned the open reading frames of the two ferritin subunits to overexpress the corresponding proteins in Escherichia coli and subsequently demonstrated their iron sequestration activity along with antibacterial activity against E. coli using respective purified recombinant proteins in vitro. A basal expression analysis of two LhFer genes in selected tissues using qPCR assays showed pronounced expression in blood cells with respect to both genes. A relatively high expression level of LhFerH was also detected in muscle tissues. The expression level of LhFer in the head kidney was significantly up-regulated following lipopolysaccharides (LPS) and Lactococcus garvieae injection. The resulting gene expression pattern upon immune stimulation suggests that ferritin may contribute to the defense against harmful pathogen infection. Collectively, our results indicate that both LhFerH and LhFerM potentially participate in the homeostasis of free Fe ions and in the host immune defense of red-lip mullet." @default.
• W4304687622 created "2022-10-12" @default.
• W4304687622 creator A5030480121 @default.
• W4304687622 creator A5039556348 @default.
• W4304687622 creator A5044058443 @default.
• W4304687622 creator A5068907947 @default.
• W4304687622 creator A5090859350 @default.
• W4304687622 date "2023-01-01" @default.
• W4304687622 modified "2023-09-26" @default.
• W4304687622 title "Molecular insights into two ferritin subunits from red-lip mullet (Liza haematocheila): Detectable antibacterial activity with its expressional response against immune stimulants" @default.
• W4304687622 cites W1960761101 @default.
• W4304687622 cites W1964111161 @default.
• W4304687622 cites W1966934571 @default.
• W4304687622 cites W1969296748 @default.
• W4304687622 cites W1972666164 @default.
• W4304687622 cites W1987119816 @default.
• W4304687622 cites W1987201769 @default.
• W4304687622 cites W1989860945 @default.
• W4304687622 cites W1989978580 @default.
• W4304687622 cites W1995587232 @default.
• W4304687622 cites W1997977268 @default.
• W4304687622 cites W2000643610 @default.
• W4304687622 cites W2002189548 @default.
• W4304687622 cites W2003090763 @default.
• W4304687622 cites W2004881490 @default.
• W4304687622 cites W2005132438 @default.
• W4304687622 cites W2006525603 @default.
• W4304687622 cites W2007097296 @default.
• W4304687622 cites W2011538447 @default.
• W4304687622 cites W2015894758 @default.
• W4304687622 cites W2018657846 @default.
• W4304687622 cites W2029915182 @default.
• W4304687622 cites W2037434347 @default.
• W4304687622 cites W2044678473 @default.
• W4304687622 cites W2048086807 @default.
• W4304687622 cites W2049373105 @default.
• W4304687622 cites W2052117680 @default.
• W4304687622 cites W2065269746 @default.
• W4304687622 cites W2069280438 @default.
• W4304687622 cites W2070766674 @default.
• W4304687622 cites W2073799621 @default.
• W4304687622 cites W2074685327 @default.
• W4304687622 cites W2077328361 @default.
• W4304687622 cites W2080621208 @default.
• W4304687622 cites W2082509603 @default.
• W4304687622 cites W2086556027 @default.
• W4304687622 cites W2086567353 @default.
• W4304687622 cites W2087244548 @default.
• W4304687622 cites W2107277218 @default.
• W4304687622 cites W2116752799 @default.
• W4304687622 cites W2117339718 @default.
• W4304687622 cites W2118745767 @default.
• W4304687622 cites W2143524974 @default.
• W4304687622 cites W2146682670 @default.
• W4304687622 cites W2157296374 @default.
• W4304687622 cites W2158115535 @default.
• W4304687622 cites W2198241891 @default.
• W4304687622 cites W2528898339 @default.
• W4304687622 cites W2616610956 @default.
• W4304687622 cites W2734149616 @default.
• W4304687622 cites W2802903477 @default.
• W4304687622 cites W2811087589 @default.
• W4304687622 cites W3019579920 @default.
• W4304687622 cites W4293247451 @default.
• W4304687622 doi "https://doi.org/10.1016/j.gene.2022.146923" @default.
• W4304687622 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36241083" @default.
• W4304687622 hasPublicationYear "2023" @default.
• W4304687622 type Work @default.
• W4304687622 citedByCount "1" @default.
• W4304687622 countsByYear W43046876222023 @default.
• W4304687622 crossrefType "journal-article" @default.
• W4304687622 hasAuthorship W4304687622A5030480121 @default.
• W4304687622 hasAuthorship W4304687622A5039556348 @default.
• W4304687622 hasAuthorship W4304687622A5044058443 @default.
• W4304687622 hasAuthorship W4304687622A5068907947 @default.
• W4304687622 hasAuthorship W4304687622A5090859350 @default.
• W4304687622 hasConcept C104292427 @default.
• W4304687622 hasConcept C104317684 @default.
• W4304687622 hasConcept C146587185 @default.
• W4304687622 hasConcept C167625842 @default.
• W4304687622 hasConcept C2778319317 @default.
• W4304687622 hasConcept C2779873506 @default.
• W4304687622 hasConcept C28452662 @default.
• W4304687622 hasConcept C47289529 @default.
• W4304687622 hasConcept C547475151 @default.
• W4304687622 hasConcept C55493867 @default.
• W4304687622 hasConcept C86803240 @default.
• W4304687622 hasConceptScore W4304687622C104292427 @default.
• W4304687622 hasConceptScore W4304687622C104317684 @default.
• W4304687622 hasConceptScore W4304687622C146587185 @default.
• W4304687622 hasConceptScore W4304687622C167625842 @default.
• W4304687622 hasConceptScore W4304687622C2778319317 @default.
• W4304687622 hasConceptScore W4304687622C2779873506 @default.
• W4304687622 hasConceptScore W4304687622C28452662 @default.
• W4304687622 hasConceptScore W4304687622C47289529 @default.
• W4304687622 hasConceptScore W4304687622C547475151 @default.
• W4304687622 hasConceptScore W4304687622C55493867 @default.
• W4304687622 hasConceptScore W4304687622C86803240 @default.

• next