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- W4304688077 endingPage "167855" @default.
- W4304688077 startingPage "167855" @default.
- W4304688077 abstract "Oligomers of the protein α-synuclein (α-syn) are thought to be a major toxic species in Parkinson's disease, particularly through their ability to permeabilize cell membranes. The green tea polyphenol epigallocatechin gallate (EGCG) has been found to reduce this ability. We have analyzed α-syn oligomer dynamics and interconversion by H/D exchange monitored by mass spectrometry (HDX-MS). Our results show that the two oligomers OI and OII co-exist in equilibrium; OI is a multimer of OII and its dissociation can be followed by HDX-MS by virtue of the correlated exchange of the N-terminal region. Urea destabilizes the α-syn oligomers, dissociating OI to OII and monomers. Oligomers exposed to EGCG undergo Met oxidation. Intriguingly, EGCG induces an oxidation-dependent effect on the structure of the N-terminal region. For the non-oxidized N-terminal region, EGCG increases the stability of the folded structure as measured by a higher level of protection against H/D exchange. In contrast, protection is clearly abrogated in the Met oxidized N-terminal region. Having a non-oxidized and disordered N-terminal region is known to be essential for efficient membrane binding. Therefore, our results suggest that the combined effect of a structural stabilization of the non-oxidized N-terminal region and the presence of a disordered oxidized N-terminal region renders the oligomers less cytotoxic by decreasing the ability of the N-terminal region to bind to cell membranes and facilitate their permeabilization." @default.
- W4304688077 created "2022-10-12" @default.
- W4304688077 creator A5051474311 @default.
- W4304688077 creator A5054620521 @default.
- W4304688077 creator A5066094184 @default.
- W4304688077 creator A5073141171 @default.
- W4304688077 date "2022-12-01" @default.
- W4304688077 modified "2023-10-18" @default.
- W4304688077 title "EGCG has Dual and Opposing Effects on the N-terminal Region of Self-associating α-synuclein Oligomers" @default.
- W4304688077 cites W1527671059 @default.
- W4304688077 cites W1834766425 @default.
- W4304688077 cites W1965888147 @default.
- W4304688077 cites W1966603445 @default.
- W4304688077 cites W1966788000 @default.
- W4304688077 cites W1985811626 @default.
- W4304688077 cites W1988244714 @default.
- W4304688077 cites W1990473626 @default.
- W4304688077 cites W1991753555 @default.
- W4304688077 cites W1992241406 @default.
- W4304688077 cites W1995191747 @default.
- W4304688077 cites W1995319721 @default.
- W4304688077 cites W2005322339 @default.
- W4304688077 cites W2005929236 @default.
- W4304688077 cites W2008818819 @default.
- W4304688077 cites W2009556855 @default.
- W4304688077 cites W2016875890 @default.
- W4304688077 cites W2020796799 @default.
- W4304688077 cites W2036203077 @default.
- W4304688077 cites W2037119711 @default.
- W4304688077 cites W2039171043 @default.
- W4304688077 cites W2041134571 @default.
- W4304688077 cites W2043517534 @default.
- W4304688077 cites W2046014998 @default.
- W4304688077 cites W2048585321 @default.
- W4304688077 cites W2048940684 @default.
- W4304688077 cites W2050786534 @default.
- W4304688077 cites W2051027493 @default.
- W4304688077 cites W2051238291 @default.
- W4304688077 cites W2055652883 @default.
- W4304688077 cites W2057358090 @default.
- W4304688077 cites W2069442155 @default.
- W4304688077 cites W2071087722 @default.
- W4304688077 cites W2073058641 @default.
- W4304688077 cites W2077556715 @default.
- W4304688077 cites W2087389265 @default.
- W4304688077 cites W2088509113 @default.
- W4304688077 cites W2089409283 @default.
- W4304688077 cites W2099611799 @default.
- W4304688077 cites W2104204507 @default.
- W4304688077 cites W2115713105 @default.
- W4304688077 cites W2118841138 @default.
- W4304688077 cites W2123822804 @default.
- W4304688077 cites W2125823338 @default.
- W4304688077 cites W2128631317 @default.
- W4304688077 cites W2130323165 @default.
- W4304688077 cites W2131918208 @default.
- W4304688077 cites W2133272695 @default.
- W4304688077 cites W2135178483 @default.
- W4304688077 cites W2136429865 @default.
- W4304688077 cites W2142038032 @default.
- W4304688077 cites W2152907257 @default.
- W4304688077 cites W2152962886 @default.
- W4304688077 cites W2155060586 @default.
- W4304688077 cites W2155356613 @default.
- W4304688077 cites W2163527448 @default.
- W4304688077 cites W2164155150 @default.
- W4304688077 cites W2164233055 @default.
- W4304688077 cites W2170036411 @default.
- W4304688077 cites W2209133573 @default.
- W4304688077 cites W2295658693 @default.
- W4304688077 cites W2314611360 @default.
- W4304688077 cites W2319161491 @default.
- W4304688077 cites W2321181342 @default.
- W4304688077 cites W2337472358 @default.
- W4304688077 cites W2417395660 @default.
- W4304688077 cites W2571594992 @default.
- W4304688077 cites W2771086331 @default.
- W4304688077 cites W2775514330 @default.
- W4304688077 cites W2794954447 @default.
- W4304688077 cites W2795236460 @default.
- W4304688077 cites W2795803259 @default.
- W4304688077 cites W2810064906 @default.
- W4304688077 cites W2885599080 @default.
- W4304688077 cites W2892126553 @default.
- W4304688077 cites W2899227006 @default.
- W4304688077 cites W2912002356 @default.
- W4304688077 cites W2914482173 @default.
- W4304688077 cites W2943436136 @default.
- W4304688077 cites W2954385672 @default.
- W4304688077 cites W2995289217 @default.
- W4304688077 cites W3003328308 @default.
- W4304688077 cites W3005252823 @default.
- W4304688077 cites W3016255521 @default.
- W4304688077 cites W3026620720 @default.
- W4304688077 cites W3033124231 @default.
- W4304688077 cites W3094959102 @default.
- W4304688077 cites W3119462174 @default.
- W4304688077 cites W3139395934 @default.