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- W4304758015 abstract "Aim: To compare the physiological behavior of mesenchymal stem/stromal cells (MSCs) within an expandable tissue-mimetic 3D system relative to in vitro expansion in a traditional 2D system. Methods: Adipose-derived MSCs (ASCs) were continuously cultured for 6 weeks on either 2D culture plastic or in a 3D hydrogel system that eliminated subculturing. ASCs were assessed for senescence, ‘stem-like’ MSC markers, and ability for their secretome to augment a secondary cell population. Results: The 3D hydrogel system resulted in an enhanced retention of more regenerative, nonsenescent ASC populations that exhibited increased expression of ‘stem-like’ MSC surface markers. Conclusion: This study introduces a proof-of-concept design for a novel modular 3D system that can improve in vitro expansion of stem-like cell populations for future regenerative therapies." @default.
- W4304758015 created "2022-10-13" @default.
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- W4304758015 date "2023-01-01" @default.
- W4304758015 modified "2023-10-01" @default.
- W4304758015 title "Novel hydrogel system eliminates subculturing and improves retention of nonsenescent mesenchymal stem cell populations" @default.
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- W4304758015 doi "https://doi.org/10.2217/rme-2022-0140" @default.
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