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- W4304944595 abstract "CRISPR effector Cas13 recognizes and degrades RNA molecules that are complementary to its guide RNA (gRNA) and possesses potential as an antiviral biotechnology because it can degrade viral mRNA and RNA genomes. Because multiplexed targeting is a critical strategy to improve viral suppression, we developed a strategy to design of gRNAs where individual gRNAs have maximized activity at multiple viral targets, simultaneously, by exploiting the molecular biophysics of promiscuous target recognition by Cas13. These polyvalent gRNA sequences (pgRNAs) provide superior antiviral elimination across tissue/organ scales in a higher organism (Nicotiana benthamiana) compared to conventionally-designed gRNAs-reducing detectable viral RNA by >30-fold, despite lacking perfect complementarity with either of their targets and, when multiplexed, reducing viral RNA by >99.5%. Pairs of pgRNA-targetable sequences are abundant in the genomes of RNA viruses, and this work highlights the need for specific approaches to the challenges of targeting viruses in eukaryotes using CRISPR." @default.
- W4304944595 created "2022-10-13" @default.
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- W4304944595 date "2022-11-01" @default.
- W4304944595 modified "2023-10-14" @default.
- W4304944595 title "Polyvalent guide RNAs for CRISPR antivirals" @default.
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- W4304944595 doi "https://doi.org/10.1016/j.isci.2022.105333" @default.
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