Matches in SemOpenAlex for { <https://semopenalex.org/work/W4305014557> ?p ?o ?g. }
- W4305014557 abstract "RNA editing converts cytidines to uridines in plant organellar transcripts. Editing typically restores codons for conserved amino acids. During evolution, specific C-to-U editing sites can be lost from some plant lineages by genomic C-to-T mutations. By contrast, the emergence of novel editing sites is less well documented. Editing sites are recognized by pentatricopeptide repeat (PPR) proteins with high specificity. RNA recognition by PPR proteins is partially predictable, but prediction is often inadequate for PPRs involved in RNA editing. Here we have characterized evolution and recognition of a recently gained editing site. We demonstrate that changes in the RNA recognition motifs that are not explainable with the current PPR code allow an ancient PPR protein, QED1, to uniquely target the ndhB-291 site in Brassicaceae. When expressed in tobacco, the Arabidopsis QED1 edits 33 high-confident off-target sites in chloroplasts and mitochondria causing a spectrum of mutant phenotypes. By manipulating the relative expression levels of QED1 and ndhB-291, we show that the target specificity of the PPR protein depends on the RNA:protein ratio. Finally, our data suggest that the low expression levels of PPR proteins are necessary to ensure the specificity of editing site selection and prevent deleterious off-target editing." @default.
- W4305014557 created "2022-10-14" @default.
- W4305014557 creator A5025931864 @default.
- W4305014557 creator A5043159071 @default.
- W4305014557 creator A5045713419 @default.
- W4305014557 creator A5047414651 @default.
- W4305014557 creator A5057388888 @default.
- W4305014557 creator A5061247186 @default.
- W4305014557 creator A5064381189 @default.
- W4305014557 creator A5075199511 @default.
- W4305014557 creator A5079298232 @default.
- W4305014557 date "2022-10-13" @default.
- W4305014557 modified "2023-09-29" @default.
- W4305014557 title "Emergence of novel RNA editing sites by changes in the binding affinity of a conserved PPR protein" @default.
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