Matches in SemOpenAlex for { <https://semopenalex.org/work/W4306150003> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W4306150003 endingPage "9" @default.
- W4306150003 startingPage "9" @default.
- W4306150003 abstract "Antigen cross-presentation is a critical process involved in the initiation of anti-viral and anti-tumour immunity. During cross-presentation, exogenous antigens are captured by dendritic cells (DCs), imported into the cytosol, and processed by proteasome for presentation on MHC class I. Our goal is to identify genes required for cross-presentation using CRISPR-Cas9 screening approaches. In order to perform pooled genetic screens, we established a range of novel assays to monitor antigen cross-presentation in single cells. To identify individual cross-presenting cells, we developed a method where cell:cell conjugates between antigen-loaded DCs and naive T cells are isolated using flow cytometry. To identify genes involved in antigen import into the cytosol specifically, we used saporin as model antigen and performed puromycin-labelling of nascent polypeptides to monitor saporin import into the cytosol and the resulting arrest in translation. As a proof of concept, we applied these assays to a heterogenous population of splenocytes and discovered that, in addition to DCs, splenic red pulp macrophages (RPMs) are also efficient in antigen import and cross-presentation. We then employed these methods in CRISPR-Cas9 pooled screens using a cDC1 cell line, MutuDCs and a custom sgRNA library targetting genes highly expressed in cross-presenting cells. We are now further characterising the hits with the overall goal to improve our understanding of mechanistic details underlying cross-presentation." @default.
- W4306150003 created "2022-10-14" @default.
- W4306150003 creator A5047963204 @default.
- W4306150003 creator A5048739218 @default.
- W4306150003 date "2022-10-01" @default.
- W4306150003 modified "2023-09-23" @default.
- W4306150003 title "CRISPR-Cas9 genetic screening approaches to uncover machinery involved in cross-presentation" @default.
- W4306150003 doi "https://doi.org/10.1016/j.molimm.2022.05.039" @default.
- W4306150003 hasPublicationYear "2022" @default.
- W4306150003 type Work @default.
- W4306150003 citedByCount "0" @default.
- W4306150003 crossrefType "journal-article" @default.
- W4306150003 hasAuthorship W4306150003A5047963204 @default.
- W4306150003 hasAuthorship W4306150003A5048739218 @default.
- W4306150003 hasConcept C104317684 @default.
- W4306150003 hasConcept C108249834 @default.
- W4306150003 hasConcept C132455925 @default.
- W4306150003 hasConcept C147483822 @default.
- W4306150003 hasConcept C170627219 @default.
- W4306150003 hasConcept C203014093 @default.
- W4306150003 hasConcept C207936829 @default.
- W4306150003 hasConcept C2776090121 @default.
- W4306150003 hasConcept C2778973524 @default.
- W4306150003 hasConcept C2908647359 @default.
- W4306150003 hasConcept C54355233 @default.
- W4306150003 hasConcept C70721500 @default.
- W4306150003 hasConcept C71924100 @default.
- W4306150003 hasConcept C83464605 @default.
- W4306150003 hasConcept C86803240 @default.
- W4306150003 hasConcept C8891405 @default.
- W4306150003 hasConcept C95444343 @default.
- W4306150003 hasConcept C98108389 @default.
- W4306150003 hasConcept C99454951 @default.
- W4306150003 hasConceptScore W4306150003C104317684 @default.
- W4306150003 hasConceptScore W4306150003C108249834 @default.
- W4306150003 hasConceptScore W4306150003C132455925 @default.
- W4306150003 hasConceptScore W4306150003C147483822 @default.
- W4306150003 hasConceptScore W4306150003C170627219 @default.
- W4306150003 hasConceptScore W4306150003C203014093 @default.
- W4306150003 hasConceptScore W4306150003C207936829 @default.
- W4306150003 hasConceptScore W4306150003C2776090121 @default.
- W4306150003 hasConceptScore W4306150003C2778973524 @default.
- W4306150003 hasConceptScore W4306150003C2908647359 @default.
- W4306150003 hasConceptScore W4306150003C54355233 @default.
- W4306150003 hasConceptScore W4306150003C70721500 @default.
- W4306150003 hasConceptScore W4306150003C71924100 @default.
- W4306150003 hasConceptScore W4306150003C83464605 @default.
- W4306150003 hasConceptScore W4306150003C86803240 @default.
- W4306150003 hasConceptScore W4306150003C8891405 @default.
- W4306150003 hasConceptScore W4306150003C95444343 @default.
- W4306150003 hasConceptScore W4306150003C98108389 @default.
- W4306150003 hasConceptScore W4306150003C99454951 @default.
- W4306150003 hasLocation W43061500031 @default.
- W4306150003 hasOpenAccess W4306150003 @default.
- W4306150003 hasPrimaryLocation W43061500031 @default.
- W4306150003 hasRelatedWork W1548452743 @default.
- W4306150003 hasRelatedWork W1680198589 @default.
- W4306150003 hasRelatedWork W1999436383 @default.
- W4306150003 hasRelatedWork W2000941383 @default.
- W4306150003 hasRelatedWork W2061503084 @default.
- W4306150003 hasRelatedWork W2069471896 @default.
- W4306150003 hasRelatedWork W2500684644 @default.
- W4306150003 hasRelatedWork W2801179043 @default.
- W4306150003 hasRelatedWork W4281479110 @default.
- W4306150003 hasRelatedWork W598549152 @default.
- W4306150003 hasVolume "150" @default.
- W4306150003 isParatext "false" @default.
- W4306150003 isRetracted "false" @default.
- W4306150003 workType "article" @default.