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- W4306167003 abstract "The necessity to preserve meniscal function prompts the research and develop¬ment of novel treatment options, like three-dimensional (3D) bioprinting. However, bioinks for meniscal 3D bioprinting have not been extensively explored. Therefore, in this study, a bioink composed of alginate, gelatin, and carboxymethylated cellu¬lose nanocrystal (CCNC) was formulated and evaluated. Firstly, bioinks with varying concentrations of the aforementioned components were subjected to rheological analysis (amplitude sweep test, temperature sweep test, and rotation). The optimal bioink formulation of 4.0% gelatin, 0.75% alginate, and 1.4% CCNC dissolved in 4.6% D-mannitol was further used for printing accuracy analysis, followed by 3D bioprint¬ing with normal human knee articular chondrocytes (NHAC-kn). The encapsulated cells’ viability was > 98%, and collagen II expression was stimulated by the bioink. The formulated bioink is printable, stable under cell culture conditions, biocompatible, and able to maintain the native phenotype of chondrocytes. Aside from meniscal tissue bioprinting, it is believed that this bioink could serve as a basis for the devel¬opment of bioinks for various tissues." @default.
- W4306167003 created "2022-10-14" @default.
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- W4306167003 date "2022-10-14" @default.
- W4306167003 modified "2023-10-17" @default.
- W4306167003 title "Formulation and evaluation of a bioink composed of alginate, gelatin, and nanocellulose for meniscal tissue engineering" @default.
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- W4306167003 doi "https://doi.org/10.18063/ijb.v9i1.621" @default.
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