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• W4306167461 endingPage "159425" @default.
• W4306167461 startingPage "159425" @default.
• W4306167461 abstract "Difenoconazole is widely used to protect crops, fruits, and vegetables. However, this fungicide can enter aquatic environments and cause harmful effects to non-target organisms and induce little-known biological disorders. Thus, aiming to expand our knowledge about the ecotoxicity of difenoconazole on freshwater ichthyofauna, we aimed to determine the median lethal concentration (LC50) of difenoconazole and evaluate its possible impacts from different toxicity biomarkers, using freshwater fish Labeo rohita as a model system. Using the probit analysis method, the 96 h LC50 value of difenoconazole in the fish was calculated as 4.5 mg L−1. Posteriorly, fish were exposed to two sublethal concentrations (0.45 mg L−1 1/10th and 0.9 mg L−1 1/5th LC50 value) for 21 days. A significant reduction of superoxide dismutase (SOD) and catalase (CAT) activity was noted in the gill, liver, and kidneys of fish compared to the control groups. The level of glutathione-S-transferase (GST) and lipid peroxidation (LPO) activity was higher in all vital tissues of difenoconazole-treated fish. Histological alterations in the gill include epithelial lifting, lamellar fusion, hypertrophy, and epithelial necrosis. At the same time, the liver showed pyknotic nucleus, vacuolation, cellular edema and tubular necrosis, shrinkage of glomeruli, vacuolation, and pyknotic nuclei in the kidney. DNA damage was increased significantly with tail formation based on the concentration and time-dependent manner. Therefore, our study confirms that the exposure of L. rohita to difenoconazole induces negative biological consequences and sheds light on the danger of this fungicide for freshwater fish species. We believe that studies like ours can support actions and strategies for the remediation/mitigation of aquatic pollution by difenoconazole and for the conservation of freshwater ichthyofauna." @default.
• W4306167461 created "2022-10-14" @default.
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• W4306167461 date "2023-01-01" @default.
• W4306167461 modified "2023-10-01" @default.
• W4306167461 title "“Fishcide” effect of the fungicide difenoconazole in freshwater fish (Labeo rohita): A multi-endpoint approach" @default.
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• W4306167461 doi "https://doi.org/10.1016/j.scitotenv.2022.159425" @default.
• W4306167461 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36244480" @default.
• W4306167461 hasPublicationYear "2023" @default.
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