Matches in SemOpenAlex for { <https://semopenalex.org/work/W4306173821> ?p ?o ?g. }
- W4306173821 endingPage "147" @default.
- W4306173821 startingPage "138" @default.
- W4306173821 abstract "Extracellular vesicles (EVs) derived from human mesenchymal stromal cells (MSCs) show immunomodulatory activity in different assays both in vitro and in vivo. In previous work, the authors compared the immunomodulatory potential of independent MSC-EV preparations in a multi-donor mixed lymphocyte reaction (mdMLR) assay and an optimized steroid-refractory acute graft-versus-host disease (aGVHD) mouse model. The authors observed that only a proportion of the MSC-EV preparations showed immunomodulatory capabilities and demonstrated that only MSC-EV preparations with mdMLR immunomodulating activities were able to suppress aGVHD symptoms in vivo and vice versa. Since the mdMLR assay is complex and depends on primary human cells of different donors, the authors sought to establish an assay that is much easier to standardize and fulfills the requirements for becoming qualified as a potency assay.The bona fide MSC antigen CD73 possesses ecto-5'-nucleotidase activity that cleaves pro-inflammatory extracellular adenosine monophosphate into anti-inflammatory adenosine and free phosphate. To test whether the ecto-5'-nucleotidase activity of the MSC-EV preparations reflected their immunomodulatory potential, the authors adopted an enzymatic assay that monitors the ecto-5'-nucleotidase activity of CD73 in a quantitative manner and compared the activity of well-characterized MSC-EV preparations containing or lacking mdMLR immunomodulatory activity.The authors showed that the ecto-5'-nucleotidase activity of the MSC-EV preparations did not correlate with their ability to modulate T-cell responses in the mdMLR assay and thus with their potency in improving disease symptomatology in the optimized mouse aGVHD model. Furthermore, the ecto-5'-nucleotidase activity was resistant to EV-destroying detergent treatment.Ecto-5'-nucleotidase activity neither reflects the potency of the authors' MSC-EV preparations nor provides any information about the integrity of the respective EVs. Thus, ecto-5'-nucleotidase enzyme activity is not indicative for the immunomodulatory potency of the authors' MSC-EV products. The development of appropriate potency assays for MSC-EV products remains challenging." @default.
- W4306173821 created "2022-10-14" @default.
- W4306173821 creator A5003700004 @default.
- W4306173821 creator A5006675461 @default.
- W4306173821 creator A5016073440 @default.
- W4306173821 creator A5028410367 @default.
- W4306173821 creator A5029778760 @default.
- W4306173821 creator A5031009228 @default.
- W4306173821 creator A5036150345 @default.
- W4306173821 creator A5049046169 @default.
- W4306173821 creator A5050887288 @default.
- W4306173821 creator A5063740884 @default.
- W4306173821 date "2023-02-01" @default.
- W4306173821 modified "2023-10-17" @default.
- W4306173821 title "CD73 activity of mesenchymal stromal cell-derived extracellular vesicle preparations is detergent-resistant and does not correlate with immunomodulatory capabilities" @default.
- W4306173821 cites W1568069191 @default.
- W4306173821 cites W1974409640 @default.
- W4306173821 cites W1979412100 @default.
- W4306173821 cites W2013847914 @default.
- W4306173821 cites W2025371271 @default.
- W4306173821 cites W2058473775 @default.
- W4306173821 cites W2060387475 @default.
- W4306173821 cites W2113045454 @default.
- W4306173821 cites W2138599948 @default.
- W4306173821 cites W2231751025 @default.
- W4306173821 cites W2296337888 @default.
- W4306173821 cites W2732667418 @default.
- W4306173821 cites W2768238755 @default.
- W4306173821 cites W2808148330 @default.
- W4306173821 cites W2897903541 @default.
- W4306173821 cites W2900756811 @default.
- W4306173821 cites W2923606319 @default.
- W4306173821 cites W2942732217 @default.
- W4306173821 cites W2972161970 @default.
- W4306173821 cites W3004044398 @default.
- W4306173821 cites W3004505539 @default.
- W4306173821 cites W3017233676 @default.
- W4306173821 cites W3025579253 @default.
- W4306173821 cites W3036417914 @default.
- W4306173821 cites W3087133836 @default.
- W4306173821 cites W3118895525 @default.
- W4306173821 cites W3154654511 @default.
- W4306173821 cites W4200071900 @default.
- W4306173821 cites W4294183732 @default.
- W4306173821 doi "https://doi.org/10.1016/j.jcyt.2022.09.006" @default.
- W4306173821 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36244910" @default.
- W4306173821 hasPublicationYear "2023" @default.
- W4306173821 type Work @default.
- W4306173821 citedByCount "6" @default.
- W4306173821 countsByYear W43061738212023 @default.
- W4306173821 crossrefType "journal-article" @default.
- W4306173821 hasAuthorship W4306173821A5003700004 @default.
- W4306173821 hasAuthorship W4306173821A5006675461 @default.
- W4306173821 hasAuthorship W4306173821A5016073440 @default.
- W4306173821 hasAuthorship W4306173821A5028410367 @default.
- W4306173821 hasAuthorship W4306173821A5029778760 @default.
- W4306173821 hasAuthorship W4306173821A5031009228 @default.
- W4306173821 hasAuthorship W4306173821A5036150345 @default.
- W4306173821 hasAuthorship W4306173821A5049046169 @default.
- W4306173821 hasAuthorship W4306173821A5050887288 @default.
- W4306173821 hasAuthorship W4306173821A5063740884 @default.
- W4306173821 hasBestOaLocation W43061738211 @default.
- W4306173821 hasConcept C104317684 @default.
- W4306173821 hasConcept C145059251 @default.
- W4306173821 hasConcept C1491633281 @default.
- W4306173821 hasConcept C150903083 @default.
- W4306173821 hasConcept C16930146 @default.
- W4306173821 hasConcept C185592680 @default.
- W4306173821 hasConcept C18880352 @default.
- W4306173821 hasConcept C198826908 @default.
- W4306173821 hasConcept C202751555 @default.
- W4306173821 hasConcept C203014093 @default.
- W4306173821 hasConcept C20518536 @default.
- W4306173821 hasConcept C207001950 @default.
- W4306173821 hasConcept C2776991684 @default.
- W4306173821 hasConcept C2777913276 @default.
- W4306173821 hasConcept C28406088 @default.
- W4306173821 hasConcept C502942594 @default.
- W4306173821 hasConcept C55493867 @default.
- W4306173821 hasConcept C86803240 @default.
- W4306173821 hasConcept C95444343 @default.
- W4306173821 hasConcept C98274493 @default.
- W4306173821 hasConceptScore W4306173821C104317684 @default.
- W4306173821 hasConceptScore W4306173821C145059251 @default.
- W4306173821 hasConceptScore W4306173821C1491633281 @default.
- W4306173821 hasConceptScore W4306173821C150903083 @default.
- W4306173821 hasConceptScore W4306173821C16930146 @default.
- W4306173821 hasConceptScore W4306173821C185592680 @default.
- W4306173821 hasConceptScore W4306173821C18880352 @default.
- W4306173821 hasConceptScore W4306173821C198826908 @default.
- W4306173821 hasConceptScore W4306173821C202751555 @default.
- W4306173821 hasConceptScore W4306173821C203014093 @default.
- W4306173821 hasConceptScore W4306173821C20518536 @default.
- W4306173821 hasConceptScore W4306173821C207001950 @default.
- W4306173821 hasConceptScore W4306173821C2776991684 @default.
- W4306173821 hasConceptScore W4306173821C2777913276 @default.
- W4306173821 hasConceptScore W4306173821C28406088 @default.
- W4306173821 hasConceptScore W4306173821C502942594 @default.