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- W4306177764 abstract "A variety of different pathogenic bacteria, viruses, and harmful algal biotoxin-producing organisms can accumulate in shellfish and other seafoods and cause a variety of human diseases. Worldwide, seafood is responsible for an important proportion of foodborne illnesses and associated outbreaks. The study of these pathogens has evolved markedly over the last two decades with the advent of molecular and genomic-based methods. Here we provide a brief overview of the most important human pathogens associated with seafood—focusing on bivalve shellfish while outlining how methods to study these pathogens have been applied and evolved in tandem with the revolution in genomic and molecular-based techniques. Certain methods, such as rapid identification methodologies including polymerase chain reaction (PCR), real-time PCR, and loop-mediated isothermal amplification, and whole-genome sequencing of pathogens of interest, have greatly altered our understanding of the pathogens responsible for seafood-associated illness. We present here some pertinent examples of how methods have evolved—particularly in the last decade—using shellfish-associated outbreak investigations that have utilized both traditional as well as “next generation” subtyping approaches. Finally, we outline novel approaches to study seafood-associated pathogens and discuss which of these techniques may be applied to study these organisms in the future." @default.
- W4306177764 created "2022-10-14" @default.
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- W4306177764 date "2023-01-01" @default.
- W4306177764 modified "2023-09-26" @default.
- W4306177764 title "The evolution of molecular methods to study seafood-associated pathogens" @default.
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- W4306177764 doi "https://doi.org/10.1016/b978-0-12-819470-6.00004-4" @default.
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