Matches in SemOpenAlex for { <https://semopenalex.org/work/W4306195276> ?p ?o ?g. }
- W4306195276 endingPage "119238" @default.
- W4306195276 startingPage "119238" @default.
- W4306195276 abstract "Free-living amoebae are ubiquitous in the environment and cause both opportunistic and non-opportunistic infections in humans. Some genera of amoebae are natural reservoirs of opportunistic plumbing pathogens, such as Legionella pneumophila. In this study, the presence of free-living amoebae and Legionella was investigated in 140 water and biofilm samples collected from Australian domestic (n = 68) and hospital water systems (n = 72). Each sample was screened in parallel using molecular and culture-based methods. Direct quantitative polymerase chain reaction (qPCR) assays showed that 41% samples were positive for Legionella, 33% for L. pneumophila, 11% for Acanthamoeba, and 55% for Vermamoeba vermiformis gene markers. Only 7% of samples contained culturable L. pneumophila serogroup (sg)1, L. pneumophila sg2-14, and non-pneumophila Legionella. In total, 69% of samples were positive for free-living amoebae using any method. Standard culturing found that 41% of the samples were positive for amoeba (either Acanthamoeba, Allovahlkampfia, Stenamoeba, or V. vermiformis). V. vermiformis showed the highest overall frequency of occurrence. Acanthamoeba and V. vermiformis isolates demonstrated high thermotolerance and osmotolerance and strong broad spectrum bacteriogenic activity against Gram-negative and Gram-positive bacteria. Importantly, all Legionella positive samples were also positive for amoeba, and this co-occurrence was statistically significant (p < 0.05). According to qPCR and fluorescence in situ hybridization, V. vermiformis and Allovahlkampfia harboured intracellular L. pneumophila. To our knowledge, this is the first time Allovahlkampfia and Stenamoeba have been demonstrated as hosts of L. pneumophila in potable water. These results demonstrate the importance of amoebae in engineered water systems, both as a pathogen and as a reservoir of Legionella. The high frequency of gymnamoebae detected in this study from Australian engineered water systems identifies an issue of significant public health concern. Future water management protocols should incorporate treatments strategies to control amoebae to reduce the risk to end users." @default.
- W4306195276 created "2022-10-14" @default.
- W4306195276 creator A5002261008 @default.
- W4306195276 creator A5022395606 @default.
- W4306195276 creator A5023290225 @default.
- W4306195276 creator A5028978450 @default.
- W4306195276 creator A5072890158 @default.
- W4306195276 creator A5074590954 @default.
- W4306195276 date "2022-11-01" @default.
- W4306195276 modified "2023-09-30" @default.
- W4306195276 title "Molecular screening and characterization of Legionella pneumophila associated free-living amoebae in domestic and hospital water systems" @default.
- W4306195276 cites W1701869895 @default.
- W4306195276 cites W1965379078 @default.
- W4306195276 cites W1967006923 @default.
- W4306195276 cites W1974186892 @default.
- W4306195276 cites W1977348564 @default.
- W4306195276 cites W1980169232 @default.
- W4306195276 cites W1980766205 @default.
- W4306195276 cites W1983289122 @default.
- W4306195276 cites W1985336221 @default.
- W4306195276 cites W1986084410 @default.
- W4306195276 cites W1997625271 @default.
- W4306195276 cites W2003341565 @default.
- W4306195276 cites W2004568202 @default.
- W4306195276 cites W2016542926 @default.
- W4306195276 cites W2017671477 @default.
- W4306195276 cites W2018789919 @default.
- W4306195276 cites W2020037351 @default.
- W4306195276 cites W2020037457 @default.
- W4306195276 cites W2035602065 @default.
- W4306195276 cites W2041256640 @default.
- W4306195276 cites W2048789295 @default.
- W4306195276 cites W2049328235 @default.
- W4306195276 cites W2062357874 @default.
- W4306195276 cites W2073890852 @default.
- W4306195276 cites W2075232887 @default.
- W4306195276 cites W2082251585 @default.
- W4306195276 cites W2088490049 @default.
- W4306195276 cites W2089337424 @default.
- W4306195276 cites W2098231476 @default.
- W4306195276 cites W2100140896 @default.
- W4306195276 cites W2100509550 @default.
- W4306195276 cites W2104752214 @default.
- W4306195276 cites W2105376921 @default.
- W4306195276 cites W2111244087 @default.
- W4306195276 cites W2112471680 @default.
- W4306195276 cites W2130278065 @default.
- W4306195276 cites W2131427475 @default.
- W4306195276 cites W2135076950 @default.
- W4306195276 cites W2151779981 @default.
- W4306195276 cites W2167658241 @default.
- W4306195276 cites W2192239723 @default.
- W4306195276 cites W2289427235 @default.
- W4306195276 cites W2332956386 @default.
- W4306195276 cites W2340415401 @default.
- W4306195276 cites W2376695922 @default.
- W4306195276 cites W2390753559 @default.
- W4306195276 cites W2411541915 @default.
- W4306195276 cites W2529963494 @default.
- W4306195276 cites W2624829897 @default.
- W4306195276 cites W2741649116 @default.
- W4306195276 cites W2766857787 @default.
- W4306195276 cites W2771040219 @default.
- W4306195276 cites W2811352542 @default.
- W4306195276 cites W2909706287 @default.
- W4306195276 cites W2915580099 @default.
- W4306195276 cites W2975783368 @default.
- W4306195276 cites W2978978722 @default.
- W4306195276 cites W3007044210 @default.
- W4306195276 cites W3009001322 @default.
- W4306195276 cites W3014044170 @default.
- W4306195276 cites W3017203947 @default.
- W4306195276 cites W3094938478 @default.
- W4306195276 cites W3163465970 @default.
- W4306195276 cites W3178493031 @default.
- W4306195276 cites W4230079656 @default.
- W4306195276 doi "https://doi.org/10.1016/j.watres.2022.119238" @default.
- W4306195276 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36270142" @default.
- W4306195276 hasPublicationYear "2022" @default.
- W4306195276 type Work @default.
- W4306195276 citedByCount "7" @default.
- W4306195276 countsByYear W43061952762023 @default.
- W4306195276 crossrefType "journal-article" @default.
- W4306195276 hasAuthorship W4306195276A5002261008 @default.
- W4306195276 hasAuthorship W4306195276A5022395606 @default.
- W4306195276 hasAuthorship W4306195276A5023290225 @default.
- W4306195276 hasAuthorship W4306195276A5028978450 @default.
- W4306195276 hasAuthorship W4306195276A5072890158 @default.
- W4306195276 hasAuthorship W4306195276A5074590954 @default.
- W4306195276 hasConcept C104317684 @default.
- W4306195276 hasConcept C2776118409 @default.
- W4306195276 hasConcept C2776172558 @default.
- W4306195276 hasConcept C2778566189 @default.
- W4306195276 hasConcept C2778868110 @default.
- W4306195276 hasConcept C2779164008 @default.
- W4306195276 hasConcept C49105822 @default.
- W4306195276 hasConcept C523546767 @default.
- W4306195276 hasConcept C54355233 @default.