Matches in SemOpenAlex for { <https://semopenalex.org/work/W4306253640> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W4306253640 endingPage "S203" @default.
- W4306253640 startingPage "S202" @default.
- W4306253640 abstract "INTRODUCTION: One of the main deleterious side effects of radiation is fibrosis of the surrounding skin and soft tissue. Clinically, this phenomenon is seen routinely and can be ameliorated with fat grafting; however, the underlying pathophysiologic changes driving this effect are not well understood. With this study, we aim to better delineate these mechanisms driving fat grafting’s regenerative effects. METHODS: Twenty adult wild-type mice underwent 5 Gy of radiation every other day for 12 days, 30 Gy total, of the dorsal skin. After 4 weeks of recovery after radiation, mice were separated into 2 treatment cohorts: fat grafted and nongrafted groups. Fat grafts were placed into the subcutaneous space of the dorsal skin. The grafts were maintained for 4 weeks and then the skin was harvested. Samples underwent single-cell RNA sequencing to identify transcriptionally distinct subpopulations and cross-referenced with Enrichr to identify relevant genetic pathways. RESULTS: Dermal thickness and collagen deposition was significantly decreased in the fat grafted cohort (Fig. A, B). Five distinct fibroblast subpopulations were identified, including inflammatory, immunomodulatory, ECM, angiogenic, and mechano-fibrotic Fig. C–E). Angiogenic fibroblasts expression was significantly enhanced with fat grafting, whereas inflammatory fibroblast expression was significantly dampened by fat grafting. Mechano-fibrotic fibroblast expression was also significantly inhibited with fat grafting. Last, the immunomodulatory fibroblast cluster had an almost 10-fold increase in expression with fat grafting (Fig. F). CONCLUSION: Modulation of these heterogenous fibroblast subpopulations with fat grafting provides insight into the cellular changes that manifest clinically in fat grafting’s ability to improve radiation-induced fibrosis.Figure" @default.
- W4306253640 created "2022-10-15" @default.
- W4306253640 creator A5004048630 @default.
- W4306253640 creator A5004377109 @default.
- W4306253640 creator A5004783535 @default.
- W4306253640 creator A5005728573 @default.
- W4306253640 creator A5025739767 @default.
- W4306253640 creator A5036888850 @default.
- W4306253640 creator A5046925887 @default.
- W4306253640 creator A5074417121 @default.
- W4306253640 creator A5089500258 @default.
- W4306253640 date "2022-10-17" @default.
- W4306253640 modified "2023-10-16" @default.
- W4306253640 title "Fibroblast Subpopulations Are Modified with Fat Grafting to Treat Radiation-Induced Fibrosis" @default.
- W4306253640 doi "https://doi.org/10.1097/01.xcs.0000894568.35895.12" @default.
- W4306253640 hasPublicationYear "2022" @default.
- W4306253640 type Work @default.
- W4306253640 citedByCount "0" @default.
- W4306253640 crossrefType "journal-article" @default.
- W4306253640 hasAuthorship W4306253640A5004048630 @default.
- W4306253640 hasAuthorship W4306253640A5004377109 @default.
- W4306253640 hasAuthorship W4306253640A5004783535 @default.
- W4306253640 hasAuthorship W4306253640A5005728573 @default.
- W4306253640 hasAuthorship W4306253640A5025739767 @default.
- W4306253640 hasAuthorship W4306253640A5036888850 @default.
- W4306253640 hasAuthorship W4306253640A5046925887 @default.
- W4306253640 hasAuthorship W4306253640A5074417121 @default.
- W4306253640 hasAuthorship W4306253640A5089500258 @default.
- W4306253640 hasBestOaLocation W43062536401 @default.
- W4306253640 hasConcept C126322002 @default.
- W4306253640 hasConcept C142724271 @default.
- W4306253640 hasConcept C171089720 @default.
- W4306253640 hasConcept C178790620 @default.
- W4306253640 hasConcept C185592680 @default.
- W4306253640 hasConcept C202751555 @default.
- W4306253640 hasConcept C2775937945 @default.
- W4306253640 hasConcept C2780381497 @default.
- W4306253640 hasConcept C2780559512 @default.
- W4306253640 hasConcept C521977710 @default.
- W4306253640 hasConcept C55493867 @default.
- W4306253640 hasConcept C71924100 @default.
- W4306253640 hasConcept C86803240 @default.
- W4306253640 hasConceptScore W4306253640C126322002 @default.
- W4306253640 hasConceptScore W4306253640C142724271 @default.
- W4306253640 hasConceptScore W4306253640C171089720 @default.
- W4306253640 hasConceptScore W4306253640C178790620 @default.
- W4306253640 hasConceptScore W4306253640C185592680 @default.
- W4306253640 hasConceptScore W4306253640C202751555 @default.
- W4306253640 hasConceptScore W4306253640C2775937945 @default.
- W4306253640 hasConceptScore W4306253640C2780381497 @default.
- W4306253640 hasConceptScore W4306253640C2780559512 @default.
- W4306253640 hasConceptScore W4306253640C521977710 @default.
- W4306253640 hasConceptScore W4306253640C55493867 @default.
- W4306253640 hasConceptScore W4306253640C71924100 @default.
- W4306253640 hasConceptScore W4306253640C86803240 @default.
- W4306253640 hasIssue "5" @default.
- W4306253640 hasLocation W43062536401 @default.
- W4306253640 hasLocation W43062536402 @default.
- W4306253640 hasOpenAccess W4306253640 @default.
- W4306253640 hasPrimaryLocation W43062536401 @default.
- W4306253640 hasRelatedWork W1977157116 @default.
- W4306253640 hasRelatedWork W2806003448 @default.
- W4306253640 hasRelatedWork W2895473996 @default.
- W4306253640 hasRelatedWork W2902432231 @default.
- W4306253640 hasRelatedWork W2973100752 @default.
- W4306253640 hasRelatedWork W3080725906 @default.
- W4306253640 hasRelatedWork W3132805953 @default.
- W4306253640 hasRelatedWork W4307985188 @default.
- W4306253640 hasRelatedWork W4318479278 @default.
- W4306253640 hasRelatedWork W4362676210 @default.
- W4306253640 hasVolume "235" @default.
- W4306253640 isParatext "false" @default.
- W4306253640 isRetracted "false" @default.
- W4306253640 workType "article" @default.