Matches in SemOpenAlex for { <https://semopenalex.org/work/W4306292093> ?p ?o ?g. }
- W4306292093 abstract "Abstract Sticholysins are α-pore-forming toxins produced by the sea-anemone Stichodactyla helianthus . These toxins exert their activity by forming pores on sphingomyelin-containing membranes. Recognition of sphingomyelin by sticholysins is required to start the process of pore formation. Sphingomyelin recognition is coupled with membrane binding and followed by membrane penetration and oligomerization. Many features of these processes are known. However, the extent of contact with each of the different kinds of lipids present in the membrane has received little attention. To delve into this question, we have used a phosphatidylcholine analogue labeled at one of its acyl chains with a doxyl moiety, a known quencher of tryptophan emission. Here we present evidence for the contact of sticholysins with phosphatidylcholine lipids in the sticholysin oligomer, and for how each sticholysin isotoxin is affected differently by the inclusion of cholesterol in the membrane. Furthermore, using phosphatidylcholine analogs that were labeled at different positions of their structure (acyl chains and headgroup) in combination with a variety of sticholysin mutants, we also investigated the depth of the tryptophan residues of sticholysins in the bilayer. Our results indicate that the position of the tryptophan residues relative to the membrane normal is deeper when cholesterol is absent from the membrane." @default.
- W4306292093 created "2022-10-15" @default.
- W4306292093 creator A5036054869 @default.
- W4306292093 creator A5047740127 @default.
- W4306292093 creator A5054271026 @default.
- W4306292093 creator A5066756492 @default.
- W4306292093 creator A5070167104 @default.
- W4306292093 creator A5071195722 @default.
- W4306292093 creator A5075323087 @default.
- W4306292093 creator A5075612265 @default.
- W4306292093 date "2022-10-15" @default.
- W4306292093 modified "2023-10-03" @default.
- W4306292093 title "Determination of the boundary lipids of sticholysins using tryptophan quenching" @default.
- W4306292093 cites W1449218956 @default.
- W4306292093 cites W145022389 @default.
- W4306292093 cites W1551656287 @default.
- W4306292093 cites W1604232295 @default.
- W4306292093 cites W1655861343 @default.
- W4306292093 cites W1670108590 @default.
- W4306292093 cites W1965114699 @default.
- W4306292093 cites W1966169046 @default.
- W4306292093 cites W1971737783 @default.
- W4306292093 cites W1973226816 @default.
- W4306292093 cites W1974478131 @default.
- W4306292093 cites W1975915958 @default.
- W4306292093 cites W1976001472 @default.
- W4306292093 cites W1979048239 @default.
- W4306292093 cites W1979722406 @default.
- W4306292093 cites W1982257706 @default.
- W4306292093 cites W1983351621 @default.
- W4306292093 cites W1984076817 @default.
- W4306292093 cites W1988181071 @default.
- W4306292093 cites W1991146845 @default.
- W4306292093 cites W1994038290 @default.
- W4306292093 cites W1994446262 @default.
- W4306292093 cites W1995725157 @default.
- W4306292093 cites W1995880118 @default.
- W4306292093 cites W2002475067 @default.
- W4306292093 cites W2003709444 @default.
- W4306292093 cites W2005040236 @default.
- W4306292093 cites W2006790257 @default.
- W4306292093 cites W2009308784 @default.
- W4306292093 cites W2013301687 @default.
- W4306292093 cites W2017698288 @default.
- W4306292093 cites W2018289835 @default.
- W4306292093 cites W2018531782 @default.
- W4306292093 cites W2020283744 @default.
- W4306292093 cites W2021551854 @default.
- W4306292093 cites W2025117402 @default.
- W4306292093 cites W2028877302 @default.
- W4306292093 cites W2038458076 @default.
- W4306292093 cites W2038497192 @default.
- W4306292093 cites W2040911397 @default.
- W4306292093 cites W2047695457 @default.
- W4306292093 cites W2049877637 @default.
- W4306292093 cites W2051673588 @default.
- W4306292093 cites W2052069688 @default.
- W4306292093 cites W2054127621 @default.
- W4306292093 cites W2054740812 @default.
- W4306292093 cites W2056235355 @default.
- W4306292093 cites W2060250212 @default.
- W4306292093 cites W2072069399 @default.
- W4306292093 cites W2076924373 @default.
- W4306292093 cites W2078759678 @default.
- W4306292093 cites W2080948385 @default.
- W4306292093 cites W2087828115 @default.
- W4306292093 cites W2090415455 @default.
- W4306292093 cites W2094095233 @default.
- W4306292093 cites W2094512218 @default.
- W4306292093 cites W2097902994 @default.
- W4306292093 cites W2099559490 @default.
- W4306292093 cites W2100837269 @default.
- W4306292093 cites W2102206503 @default.
- W4306292093 cites W2103725795 @default.
- W4306292093 cites W2120363432 @default.
- W4306292093 cites W2124634836 @default.
- W4306292093 cites W2129388681 @default.
- W4306292093 cites W2132668893 @default.
- W4306292093 cites W2134068468 @default.
- W4306292093 cites W2138993728 @default.
- W4306292093 cites W2143761147 @default.
- W4306292093 cites W2155195157 @default.
- W4306292093 cites W2155921993 @default.
- W4306292093 cites W2159152993 @default.
- W4306292093 cites W2164186503 @default.
- W4306292093 cites W2168201096 @default.
- W4306292093 cites W2195753589 @default.
- W4306292093 cites W2213825066 @default.
- W4306292093 cites W2293442890 @default.
- W4306292093 cites W2306939891 @default.
- W4306292093 cites W2317164129 @default.
- W4306292093 cites W2333308840 @default.
- W4306292093 cites W2491458126 @default.
- W4306292093 cites W2548773199 @default.
- W4306292093 cites W2552941677 @default.
- W4306292093 cites W2758552779 @default.
- W4306292093 cites W2769587033 @default.
- W4306292093 cites W2804104118 @default.
- W4306292093 cites W2911712006 @default.
- W4306292093 cites W2914316268 @default.