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- W4306353454 abstract "ABSTRACT Methods for creating seamless genome modifications are an essential part of the microbial genetic toolkit that allows for strain engineering through the recycling of selectable markers. Here, we report the development of a method, termed SLICER, which can be used to create seamless genome modifications in D. radiodurans . We used SLICER to sequentially target four putative restriction-modification (R-M) system genes, recycling the same selective and screening markers for each subsequent deletion. A fifth R-M gene was replaced by a selectable marker to create a final D. radiodurans strain with 5 of the 6 putative R-M systems deleted. While we observed no significant increase in transformation efficiency, SLICER is a promising method to obtain a fully restriction-minus strain and expand the synthetic biology applications of D. radiodurans including as an in vivo DNA assembly platform. GRAPHICAL ABSTRACT" @default.
- W4306353454 created "2022-10-16" @default.
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- W4306353454 date "2022-10-16" @default.
- W4306353454 modified "2023-09-28" @default.
- W4306353454 title "SLICER: Seamless Loss of Integrated Cassettes Using Endonuclease Cleavage and Recombination in<i>Deinococcus radiodurans</i>" @default.
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- W4306353454 doi "https://doi.org/10.1101/2022.10.15.512367" @default.
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