Matches in SemOpenAlex for { <https://semopenalex.org/work/W4306366845> ?p ?o ?g. }
- W4306366845 endingPage "3241" @default.
- W4306366845 startingPage "3241" @default.
- W4306366845 abstract "Cell replacement therapies may be key in achieving functional recovery in neurodegenerative optic neuropathies diseases such as glaucoma. One strategy that holds promise in this regard is the use of human embryonic stem cell and induced pluripotent stem-derived retinal ganglion cells (hRGCs). Previous hRGC transplantation studies have shown modest success. This is in part due to the low survival and integration of the transplanted cells in the host retina. The field is further challenged by mixed assays and outcome measurements that probe and determine transplantation success. Thefore, we have devised a transplantation assay involving hRGCs and mouse retina explants that bypasses physical barriers imposed by retinal membranes. We show that hRGC neurites and somas are capable of invading mouse explants with a subset of hRGC neurites being guided by mouse RGC axons. Neonatal mouse retina explants, and to a lesser extent, adult explants, promote hRGC integrity and neurite outgrowth. Using this assay, we tested whether suppmenting cultures with brain derived neurotrophic factor (BDNF) and the adenylate cyclase activator, forskolin, enhances hRGC neurite integration, neurite outgrowth, and integrity. We show that supplementing cultures with a combination BDNF and forskolin strongly favors hRGC integrity, increasing neurite outgrowth and complexity as well as the invasion of mouse explants. The transplantation assay presented here is a practical tool for investigating strategies for testing and optimizing the integration of donor cells into host tissues." @default.
- W4306366845 created "2022-10-17" @default.
- W4306366845 creator A5010200894 @default.
- W4306366845 creator A5035743592 @default.
- W4306366845 creator A5040841311 @default.
- W4306366845 creator A5044389200 @default.
- W4306366845 creator A5048089471 @default.
- W4306366845 creator A5051128521 @default.
- W4306366845 creator A5076526686 @default.
- W4306366845 date "2022-10-15" @default.
- W4306366845 modified "2023-10-11" @default.
- W4306366845 title "Ex Vivo Integration of Human Stem Retinal Ganglion Cells into the Mouse Retina" @default.
- W4306366845 cites W1562429511 @default.
- W4306366845 cites W1969723032 @default.
- W4306366845 cites W1984418778 @default.
- W4306366845 cites W2019262432 @default.
- W4306366845 cites W2027504059 @default.
- W4306366845 cites W2027846728 @default.
- W4306366845 cites W2041612070 @default.
- W4306366845 cites W2056475801 @default.
- W4306366845 cites W2057879920 @default.
- W4306366845 cites W2101365155 @default.
- W4306366845 cites W2128749718 @default.
- W4306366845 cites W2131095682 @default.
- W4306366845 cites W2143189496 @default.
- W4306366845 cites W2158718046 @default.
- W4306366845 cites W2160605010 @default.
- W4306366845 cites W2164591831 @default.
- W4306366845 cites W2165908983 @default.
- W4306366845 cites W2318035006 @default.
- W4306366845 cites W2409724652 @default.
- W4306366845 cites W2460681702 @default.
- W4306366845 cites W2531683815 @default.
- W4306366845 cites W2580292590 @default.
- W4306366845 cites W2761739377 @default.
- W4306366845 cites W2763984188 @default.
- W4306366845 cites W2770690804 @default.
- W4306366845 cites W2894302604 @default.
- W4306366845 cites W2899595347 @default.
- W4306366845 cites W2952325380 @default.
- W4306366845 cites W2966692055 @default.
- W4306366845 cites W2984519822 @default.
- W4306366845 cites W3107281376 @default.
- W4306366845 cites W3108729425 @default.
- W4306366845 cites W3112042299 @default.
- W4306366845 cites W3112356895 @default.
- W4306366845 cites W3116618454 @default.
- W4306366845 cites W3131400436 @default.
- W4306366845 cites W3159640149 @default.
- W4306366845 cites W3171993855 @default.
- W4306366845 cites W3192931997 @default.
- W4306366845 cites W4206485229 @default.
- W4306366845 cites W4211089624 @default.
- W4306366845 cites W4220937678 @default.
- W4306366845 cites W4224099117 @default.
- W4306366845 cites W4281779507 @default.
- W4306366845 doi "https://doi.org/10.3390/cells11203241" @default.
- W4306366845 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36291110" @default.
- W4306366845 hasPublicationYear "2022" @default.
- W4306366845 type Work @default.
- W4306366845 citedByCount "4" @default.
- W4306366845 countsByYear W43063668452023 @default.
- W4306366845 crossrefType "journal-article" @default.
- W4306366845 hasAuthorship W4306366845A5010200894 @default.
- W4306366845 hasAuthorship W4306366845A5035743592 @default.
- W4306366845 hasAuthorship W4306366845A5040841311 @default.
- W4306366845 hasAuthorship W4306366845A5044389200 @default.
- W4306366845 hasAuthorship W4306366845A5048089471 @default.
- W4306366845 hasAuthorship W4306366845A5051128521 @default.
- W4306366845 hasAuthorship W4306366845A5076526686 @default.
- W4306366845 hasBestOaLocation W43063668451 @default.
- W4306366845 hasConcept C104317684 @default.
- W4306366845 hasConcept C107459253 @default.
- W4306366845 hasConcept C113246987 @default.
- W4306366845 hasConcept C126322002 @default.
- W4306366845 hasConcept C145103041 @default.
- W4306366845 hasConcept C150903083 @default.
- W4306366845 hasConcept C153911025 @default.
- W4306366845 hasConcept C160539049 @default.
- W4306366845 hasConcept C169760540 @default.
- W4306366845 hasConcept C170493617 @default.
- W4306366845 hasConcept C202751555 @default.
- W4306366845 hasConcept C207001950 @default.
- W4306366845 hasConcept C26291073 @default.
- W4306366845 hasConcept C2777093970 @default.
- W4306366845 hasConcept C2777624874 @default.
- W4306366845 hasConcept C2780827179 @default.
- W4306366845 hasConcept C28328180 @default.
- W4306366845 hasConcept C2911091166 @default.
- W4306366845 hasConcept C55493867 @default.
- W4306366845 hasConcept C71924100 @default.
- W4306366845 hasConcept C86803240 @default.
- W4306366845 hasConcept C95444343 @default.
- W4306366845 hasConceptScore W4306366845C104317684 @default.
- W4306366845 hasConceptScore W4306366845C107459253 @default.
- W4306366845 hasConceptScore W4306366845C113246987 @default.
- W4306366845 hasConceptScore W4306366845C126322002 @default.
- W4306366845 hasConceptScore W4306366845C145103041 @default.