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- W4306641915 abstract "Exocytosis plays an important role in plant-microbe interactions, in both pathogenesis and symbiosis. Exo70 proteins are integral components of the exocyst, an octameric complex that mediates tethering of vesicles to membranes in eukaryotes. Although plant Exo70s are known to be targeted by pathogen effectors, the underpinning molecular mechanisms and the impact of this interaction on infection are poorly understood. Here, we show the molecular basis of the association between the effector AVR-Pii of the blast fungus Maganaporthe oryzae and rice Exo70 alleles OsExo70F2 and OsExo70F3, which is sensed by the immune receptor pair Pii via an integrated RIN4/NOI domain. The crystal structure of AVR-Pii in complex with OsExo70F2 reveals that the effector binds to a conserved hydrophobic pocket in Exo70, defining an effector/target binding interface. Structure-guided and random mutagenesis validates the importance of AVR-Pii residues at the Exo70 binding interface to sustain protein association and disease resistance in rice when challenged with fungal strains expressing effector mutants. Furthermore, the structure of AVR-Pii defines a zinc-finger effector fold (ZiF) distinct from the MAX (Magnaporthe Avrs and ToxB-like) fold previously described for a majority of characterized M. oryzae effectors. Our data suggest that blast fungus ZiF effectors bind a conserved Exo70 interface to manipulate plant exocytosis and that these effectors are also baited by plant immune receptors, pointing to new opportunities for engineering disease resistance." @default.
- W4306641915 created "2022-10-18" @default.
- W4306641915 creator A5004338192 @default.
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- W4306641915 date "2022-10-17" @default.
- W4306641915 modified "2023-10-06" @default.
- W4306641915 title "A blast fungus zinc-finger fold effector binds to a hydrophobic pocket in host Exo70 proteins to modulate immune recognition in rice" @default.
- W4306641915 cites W1502336081 @default.
- W4306641915 cites W1508614407 @default.
- W4306641915 cites W1560516060 @default.
- W4306641915 cites W1586396057 @default.
- W4306641915 cites W1773424352 @default.
- W4306641915 cites W1965366405 @default.
- W4306641915 cites W1979796171 @default.
- W4306641915 cites W1998671658 @default.
- W4306641915 cites W1999214874 @default.
- W4306641915 cites W2011314862 @default.
- W4306641915 cites W2029835563 @default.
- W4306641915 cites W2040845455 @default.
- W4306641915 cites W2053275587 @default.
- W4306641915 cites W2058198287 @default.
- W4306641915 cites W2064903987 @default.
- W4306641915 cites W2079684828 @default.
- W4306641915 cites W2087157154 @default.
- W4306641915 cites W2110643324 @default.
- W4306641915 cites W2114776285 @default.
- W4306641915 cites W2128029989 @default.
- W4306641915 cites W2129059025 @default.
- W4306641915 cites W2134233898 @default.
- W4306641915 cites W2144998676 @default.
- W4306641915 cites W2146059247 @default.
- W4306641915 cites W2146949085 @default.
- W4306641915 cites W2157502333 @default.
- W4306641915 cites W2158924989 @default.
- W4306641915 cites W2162665092 @default.
- W4306641915 cites W2177524008 @default.
- W4306641915 cites W2179901684 @default.
- W4306641915 cites W2224298134 @default.
- W4306641915 cites W2279882219 @default.
- W4306641915 cites W2286410612 @default.
- W4306641915 cites W2376573086 @default.
- W4306641915 cites W2463928272 @default.
- W4306641915 cites W2577995238 @default.
- W4306641915 cites W2581461850 @default.
- W4306641915 cites W2590735293 @default.
- W4306641915 cites W2598527232 @default.
- W4306641915 cites W2600568133 @default.
- W4306641915 cites W2608723263 @default.
- W4306641915 cites W2747555641 @default.
- W4306641915 cites W2781719859 @default.
- W4306641915 cites W2783331331 @default.
- W4306641915 cites W2783705795 @default.
- W4306641915 cites W2783965229 @default.
- W4306641915 cites W2790329381 @default.
- W4306641915 cites W2793991964 @default.
- W4306641915 cites W2814127638 @default.
- W4306641915 cites W2865801967 @default.
- W4306641915 cites W2896861545 @default.
- W4306641915 cites W2920570109 @default.
- W4306641915 cites W2951870836 @default.
- W4306641915 cites W2953178252 @default.
- W4306641915 cites W2961711147 @default.
- W4306641915 cites W2971641771 @default.
- W4306641915 cites W2973396428 @default.
- W4306641915 cites W2977192640 @default.
- W4306641915 cites W3051231894 @default.
- W4306641915 cites W3083406432 @default.
- W4306641915 cites W3092501779 @default.
- W4306641915 cites W3106735838 @default.
- W4306641915 cites W3124505447 @default.
- W4306641915 cites W3125340241 @default.
- W4306641915 cites W3128262323 @default.
- W4306641915 cites W3134990295 @default.
- W4306641915 cites W3165486161 @default.
- W4306641915 cites W3177828909 @default.
- W4306641915 cites W3196518801 @default.
- W4306641915 cites W3199291386 @default.
- W4306641915 cites W3207986610 @default.
- W4306641915 cites W3210729941 @default.
- W4306641915 cites W3213786893 @default.
- W4306641915 cites W4220905794 @default.
- W4306641915 cites W4226282791 @default.
- W4306641915 cites W4233003322 @default.
- W4306641915 cites W4281382582 @default.
- W4306641915 cites W4281790889 @default.
- W4306641915 cites W4282981730 @default.
- W4306641915 cites W4283826677 @default.
- W4306641915 doi "https://doi.org/10.1073/pnas.2210559119" @default.
- W4306641915 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36252011" @default.
- W4306641915 hasPublicationYear "2022" @default.
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