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- W4306648534 abstract "Abstract Small open reading frames (sORFs) have been reported to play important roles in growth, regulation of morphogenesis, and abiotic stress responses in various plant species. However, their sequences and functions remain poorly understood in many plant species including Cucumis sativus. Cucumis sativus (commonly known as cucumber) is Asia’s fourth most important vegetable and the second most important crop in Western Europe. The breeding of climate-resilient cucumbers is of great importance to ensure their sustainability under extreme climate conditions. In this study, we aim to isolate the intergenic sORFs from C. sativus var. hardwickii genome and determine their sequence diversity and expression profiles in C. sativus var. hardwickii and different cultivars of C. sativus var. sativus using bioinformatics tools. We identified a total of 50,191 coding sORFs with coding potential (coding sORFs) from C. sativus var. hardwickii genome. In addition, 1,311 transcribed sORFs were detected in RNA-seq datasets of C. sativus var. hardwickii and shared homology to sequences deposited in the cucumber EST database, and among these, 91 transcribed sORFs with translation potential were detected. A total of 629 high-confident C. sativus -specific sORFs were identified in both varieties. Varietal-specific transcribed sORFs were also identified in C. sativus var. hardwickii (87) and C. sativus var. sativus (2,906). Furthermore, cultivar- and tissue-specific transcribed sORFs were identified in different cultivars and tissue samples. The findings of this study provide insight into sequence diversity and expression patterns of sORFs in C. sativus , which could help in developing climate-resilient cucumbers." @default.
- W4306648534 created "2022-10-18" @default.
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- W4306648534 date "2022-10-17" @default.
- W4306648534 modified "2023-09-26" @default.
- W4306648534 title "<i>In silico</i>comparative RNA-seq analysis reveals varietal-specific intergenic small open reading frames in<i>Cucumis sativus</i>L." @default.
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- W4306648534 doi "https://doi.org/10.1101/2022.10.13.512045" @default.
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