Matches in SemOpenAlex for { <https://semopenalex.org/work/W4306672922> ?p ?o ?g. }
- W4306672922 abstract "Integration of mechanical cues in conventional 2D or 3D cell culture platforms is an important consideration for in vivo and ex vivo models of lung health and disease. Available commercial and published custom-made devices are frequently limited in breadth of applications, scalability, and customization. Herein we present a technical report on an open-source, cell and tissue (CaT) stretcher, with modularity for different in vitro and ex vivo systems, that includes the following features: 1) Programmability for modeling different breathing patterns, 2) scalability to support low to high-throughput experimentation, and 3) modularity for submerged cell culture, organ-on-chips, hydrogels, and live tissues. The strategy for connecting the experimental cell or tissue samples to the stretching device were designed to ensure that traditional biomedical outcome measurements including, but not limited to microscopy, soluble mediator measurement, and gene and protein expression remained possible. Lastly, to increase the uptake of the device within the community, the system was built with economically feasible and available components. To accommodate diverse in vitro and ex vivo model systems we developed a variety of chips made of compliant polydimethylsiloxane (PDMS) and optimized coating strategies to increase cell adherence and viability during stretch. The CaT stretcher was validated for studying mechanotransduction pathways in lung cells and tissues, with an increase in alpha smooth muscle actin protein following stretch for 24 h observed in independent submerged monolayer, 3D hydrogel, and live lung tissue experiments. We anticipate that the open-source CaT stretcher design will increase accessibility to studies of the dynamic lung microenvironment through direct implementation by other research groups or custom iterations on our designs." @default.
- W4306672922 created "2022-10-19" @default.
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- W4306672922 date "2022-10-18" @default.
- W4306672922 modified "2023-10-17" @default.
- W4306672922 title "The CaT stretcher: An open-source system for delivering uniaxial strain to cells and tissues (CaT)" @default.
- W4306672922 cites W1576773659 @default.
- W4306672922 cites W1802322664 @default.
- W4306672922 cites W1921845417 @default.
- W4306672922 cites W1969049128 @default.
- W4306672922 cites W1969955215 @default.
- W4306672922 cites W1983639828 @default.
- W4306672922 cites W1995592152 @default.
- W4306672922 cites W1996906010 @default.
- W4306672922 cites W2004829388 @default.
- W4306672922 cites W2025522134 @default.
- W4306672922 cites W2031938767 @default.
- W4306672922 cites W2038798818 @default.
- W4306672922 cites W2051513660 @default.
- W4306672922 cites W2057629069 @default.
- W4306672922 cites W2058039595 @default.
- W4306672922 cites W2062461864 @default.
- W4306672922 cites W2076922024 @default.
- W4306672922 cites W2078205253 @default.
- W4306672922 cites W2086166247 @default.
- W4306672922 cites W2112404993 @default.
- W4306672922 cites W2116043657 @default.
- W4306672922 cites W2118997818 @default.
- W4306672922 cites W2131159715 @default.
- W4306672922 cites W2135514741 @default.
- W4306672922 cites W2146337514 @default.
- W4306672922 cites W2146883927 @default.
- W4306672922 cites W2153010576 @default.
- W4306672922 cites W2159785500 @default.
- W4306672922 cites W2168072233 @default.
- W4306672922 cites W2174788854 @default.
- W4306672922 cites W2256963343 @default.
- W4306672922 cites W2260133387 @default.
- W4306672922 cites W2460939637 @default.
- W4306672922 cites W2492011816 @default.
- W4306672922 cites W2597070792 @default.
- W4306672922 cites W2616699005 @default.
- W4306672922 cites W2713709209 @default.
- W4306672922 cites W2778403639 @default.
- W4306672922 cites W2790455901 @default.
- W4306672922 cites W2796867306 @default.
- W4306672922 cites W2804064570 @default.
- W4306672922 cites W2806674349 @default.
- W4306672922 cites W2883787173 @default.
- W4306672922 cites W2884435515 @default.
- W4306672922 cites W2896782101 @default.
- W4306672922 cites W2916127417 @default.
- W4306672922 cites W2941683871 @default.
- W4306672922 cites W2953914728 @default.
- W4306672922 cites W2959654103 @default.
- W4306672922 cites W2961583814 @default.
- W4306672922 cites W2979745944 @default.
- W4306672922 cites W2981228580 @default.
- W4306672922 cites W2994686491 @default.
- W4306672922 cites W3002549336 @default.
- W4306672922 cites W3005768133 @default.
- W4306672922 cites W3012646569 @default.
- W4306672922 cites W3033829129 @default.
- W4306672922 cites W3046714906 @default.
- W4306672922 cites W3049638334 @default.
- W4306672922 cites W3093185654 @default.
- W4306672922 cites W3112703389 @default.
- W4306672922 cites W3123938533 @default.
- W4306672922 cites W3126540362 @default.
- W4306672922 cites W3127810338 @default.
- W4306672922 cites W3154399372 @default.
- W4306672922 cites W3157186884 @default.
- W4306672922 cites W3157288445 @default.
- W4306672922 cites W3165992805 @default.
- W4306672922 cites W3175201732 @default.
- W4306672922 cites W3178577242 @default.
- W4306672922 cites W4221110509 @default.
- W4306672922 cites W4225500737 @default.
- W4306672922 doi "https://doi.org/10.3389/fbioe.2022.959335" @default.
- W4306672922 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36329705" @default.
- W4306672922 hasPublicationYear "2022" @default.
- W4306672922 type Work @default.