FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4306704105> ?p ?o ?g. }

• W4306704105 abstract "Urinary tract infections (UTIs) pose a major public health burden. The vast majority of UTIs are caused by Gram-negative bacteria. Current culture-based pathogen identification methods may require up to 24 to 48 h of incubation. In this study, we developed and evaluated a method for Gram-negative pathogen identification direct from urine, without culture, via matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) in approximately 1 h. Urine samples were collected (n = 137) from the University of Maryland Medical Center clinical microbiology laboratory. To identify bacteria direct from urine, two methods were evaluated. First, 1 μL of urine was directly spotted onto the MALDI target plate, and second, 1 mL of urine was centrifuged at 8,000 rpm for 5 min before processing using the fast lipid analysis technique (FLAT). Mass spectra were acquired on the Bruker MALDI Biotyper sirius system in the negative-ion mode. Results were compared to those of standard culture methods. When 1 μL of urine was directly spotted, positive agreement was 81.5% (101/124) and, after centrifugation, 94.4% (117/124) relative to that of standard culture methods. Negative agreement for both methods was 100% (13/13). The time to results for both of the specimen preparation methods using the FLAT extraction protocol was approximately 1 h, with minimal hands-on time required (<5 min). The ability to rapidly identify pathogens directly from urine, without the need for culture, allows for faster turnaround times and, potentially, improved patient outcomes. Overall, the FLAT extraction protocol, in combination with lipid A identification, provides a reproducible and accurate method to rapidly identify urinary pathogens. IMPORTANCE This study describes and evaluates a direct-from-urine extraction method that allows identification of Gram-negative bacteria via MALDI-TOF MS within 1 h. Currently, identification of urinary pathogens requires 24 h of culture prior to identification. While this method may not replace culture, we demonstrate its utility in screening for common urinary pathogens. By providing identifications in under 1 h, clinicians can potentially treat patients sooner with more-targeted antimicrobial therapy. In turn, earlier treatment can improve patient outcome and antimicrobial stewardship. Furthermore, MADLI-TOF MS is a readily available, easy-to-use diagnostic tool in clinical laboratories, making implementation of this method possible." @default.
• W4306704105 created "2022-10-19" @default.
• W4306704105 creator A5005038707 @default.
• W4306704105 creator A5008315421 @default.
• W4306704105 creator A5017969903 @default.
• W4306704105 creator A5023542709 @default.
• W4306704105 creator A5034985119 @default.
• W4306704105 creator A5043813423 @default.
• W4306704105 date "2022-12-21" @default.
• W4306704105 modified "2023-09-30" @default.
• W4306704105 title "A Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Direct-from-Urine-Specimen Diagnostic for Gram-Negative Pathogens" @default.
• W4306704105 cites W1511667485 @default.
• W4306704105 cites W1645619903 @default.
• W4306704105 cites W1805560940 @default.
• W4306704105 cites W1968932738 @default.
• W4306704105 cites W1975367242 @default.
• W4306704105 cites W2013637885 @default.
• W4306704105 cites W2021431692 @default.
• W4306704105 cites W2031240692 @default.
• W4306704105 cites W2031763940 @default.
• W4306704105 cites W2063356448 @default.
• W4306704105 cites W2072284217 @default.
• W4306704105 cites W2100073472 @default.
• W4306704105 cites W2112264573 @default.
• W4306704105 cites W2119918510 @default.
• W4306704105 cites W2155800098 @default.
• W4306704105 cites W2155860425 @default.
• W4306704105 cites W2163186898 @default.
• W4306704105 cites W2172112723 @default.
• W4306704105 cites W2179036997 @default.
• W4306704105 cites W2189060050 @default.
• W4306704105 cites W2604518601 @default.
• W4306704105 cites W2619162616 @default.
• W4306704105 cites W2737314490 @default.
• W4306704105 cites W2746249114 @default.
• W4306704105 cites W2903295599 @default.
• W4306704105 cites W2903491237 @default.
• W4306704105 cites W2905932961 @default.
• W4306704105 cites W2915041558 @default.
• W4306704105 cites W2918647516 @default.
• W4306704105 cites W2967890395 @default.
• W4306704105 cites W2979566712 @default.
• W4306704105 cites W3109474890 @default.
• W4306704105 cites W3111179909 @default.
• W4306704105 cites W3111872950 @default.
• W4306704105 cites W3196187656 @default.
• W4306704105 cites W3212923234 @default.
• W4306704105 cites W4200351956 @default.
• W4306704105 cites W4210528702 @default.
• W4306704105 doi "https://doi.org/10.1128/spectrum.03730-22" @default.
• W4306704105 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36255333" @default.
• W4306704105 hasPublicationYear "2022" @default.
• W4306704105 type Work @default.
• W4306704105 citedByCount "1" @default.
• W4306704105 countsByYear W43067041052023 @default.
• W4306704105 crossrefType "journal-article" @default.
• W4306704105 hasAuthorship W4306704105A5005038707 @default.
• W4306704105 hasAuthorship W4306704105A5008315421 @default.
• W4306704105 hasAuthorship W4306704105A5017969903 @default.
• W4306704105 hasAuthorship W4306704105A5023542709 @default.
• W4306704105 hasAuthorship W4306704105A5034985119 @default.
• W4306704105 hasAuthorship W4306704105A5043813423 @default.
• W4306704105 hasBestOaLocation W43067041051 @default.
• W4306704105 hasConcept C126322002 @default.
• W4306704105 hasConcept C162356407 @default.
• W4306704105 hasConcept C185592680 @default.
• W4306704105 hasConcept C2779225474 @default.
• W4306704105 hasConcept C2780026642 @default.
• W4306704105 hasConcept C43617362 @default.
• W4306704105 hasConcept C71924100 @default.
• W4306704105 hasConcept C86803240 @default.
• W4306704105 hasConcept C89423630 @default.
• W4306704105 hasConceptScore W4306704105C126322002 @default.
• W4306704105 hasConceptScore W4306704105C162356407 @default.
• W4306704105 hasConceptScore W4306704105C185592680 @default.
• W4306704105 hasConceptScore W4306704105C2779225474 @default.
• W4306704105 hasConceptScore W4306704105C2780026642 @default.
• W4306704105 hasConceptScore W4306704105C43617362 @default.
• W4306704105 hasConceptScore W4306704105C71924100 @default.
• W4306704105 hasConceptScore W4306704105C86803240 @default.
• W4306704105 hasConceptScore W4306704105C89423630 @default.
• W4306704105 hasFunder F4320309123 @default.
• W4306704105 hasIssue "6" @default.
• W4306704105 hasLocation W43067041051 @default.
• W4306704105 hasLocation W43067041052 @default.
• W4306704105 hasLocation W43067041053 @default.
• W4306704105 hasOpenAccess W4306704105 @default.
• W4306704105 hasPrimaryLocation W43067041051 @default.
• W4306704105 hasRelatedWork W2392924105 @default.
• W4306704105 hasRelatedWork W2394092719 @default.
• W4306704105 hasRelatedWork W2539168248 @default.
• W4306704105 hasRelatedWork W2748952813 @default.
• W4306704105 hasRelatedWork W2772070920 @default.
• W4306704105 hasRelatedWork W2783350430 @default.
• W4306704105 hasRelatedWork W2899084033 @default.
• W4306704105 hasRelatedWork W2971700887 @default.
• W4306704105 hasRelatedWork W3173126061 @default.
• W4306704105 hasRelatedWork W4323288223 @default.
• W4306704105 hasVolume "10" @default.
• W4306704105 isParatext "false" @default.

• next