FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4306811590> ?p ?o ?g. }

• W4306811590 endingPage "101" @default.
• W4306811590 startingPage "88" @default.
• W4306811590 abstract "Selective targeting of germline B cells with specifically designed germline-targeting HIV-1 envelope immunogens (GT-Env) is considered a feasible vaccination strategy to elicit broadly neutralizing antibodies (bnAbs). BnAbs are extremely valuable because they neutralize genetically distant viral strains at the same time. To overcome its inherently low affinity to germline B cells, the aim of the study was to present GT-Env via different immobilization strategies densely arrayed on the surface of nanoparticles. We engineered a prefusion-stabilized GT-Env trimer with affinity to VRC01 germline B cells using a bioinformatics-supported design approach. Distinct glycan modifications and amino acid substitutions yielded a GT-Env trimer which bound to the receptor with a KD of 11.5 µM. Silica nanoparticles with 200 nm diameter (SiNPs) were used for the multivalent display of the novel GT-Env with a 15 nm mean centre-to-centre spacing either by site-specific, covalent conjugation or at random, non-specific adsorption. Oriented, covalent GT-Env conjugation revealed better binding of structure dependent bnAbs as compared to non-specifically adsorbed GT-Env. In addition, GT-Env covalently attached activated a B cell line expressing the germline VRC01 receptor at an EC50 value in the nanomolar range (4 nM), while soluble GT-Env required 1,000-fold higher concentrations to induce signalling. The significantly lower GT-Env concentration was likely required due to avidity effects, which were in the picomolar range. Thus, low affinity antigens may particularly benefit from a particulate and multivalent delivery. In future, SiNPs are ideal to be modified in a modular design with various GT-Env variants that target different stages of germline and bnAb precursor B cells." @default.
• W4306811590 created "2022-10-20" @default.
• W4306811590 creator A5000276308 @default.
• W4306811590 creator A5001634837 @default.
• W4306811590 creator A5002078560 @default.
• W4306811590 creator A5006775077 @default.
• W4306811590 creator A5009283691 @default.
• W4306811590 creator A5010962396 @default.
• W4306811590 creator A5020871813 @default.
• W4306811590 creator A5072870154 @default.
• W4306811590 creator A5075966059 @default.
• W4306811590 creator A5088212001 @default.
• W4306811590 date "2022-12-01" @default.
• W4306811590 modified "2023-10-07" @default.
• W4306811590 title "Multivalent display of engineered HIV-1 envelope trimers on silica nanoparticles for targeting and in vitro activation of germline VRC01 B cells" @default.
• W4306811590 cites W1533750546 @default.
• W4306811590 cites W1785108662 @default.
• W4306811590 cites W1915453701 @default.
• W4306811590 cites W1968360645 @default.
• W4306811590 cites W1971777539 @default.
• W4306811590 cites W1978913715 @default.
• W4306811590 cites W1989468850 @default.
• W4306811590 cites W2006458110 @default.
• W4306811590 cites W2016453064 @default.
• W4306811590 cites W2029135056 @default.
• W4306811590 cites W2036524475 @default.
• W4306811590 cites W2052937459 @default.
• W4306811590 cites W2096406493 @default.
• W4306811590 cites W2108868448 @default.
• W4306811590 cites W2114716910 @default.
• W4306811590 cites W2122617162 @default.
• W4306811590 cites W2124445678 @default.
• W4306811590 cites W2125878189 @default.
• W4306811590 cites W2130479394 @default.
• W4306811590 cites W2145909184 @default.
• W4306811590 cites W2155201844 @default.
• W4306811590 cites W2158417216 @default.
• W4306811590 cites W2163407803 @default.
• W4306811590 cites W2167423357 @default.
• W4306811590 cites W2199211191 @default.
• W4306811590 cites W2302583067 @default.
• W4306811590 cites W2381153372 @default.
• W4306811590 cites W2515956381 @default.
• W4306811590 cites W2520562417 @default.
• W4306811590 cites W2580067296 @default.
• W4306811590 cites W2581209976 @default.
• W4306811590 cites W2594897395 @default.
• W4306811590 cites W2607794913 @default.
• W4306811590 cites W2738411688 @default.
• W4306811590 cites W2751727630 @default.
• W4306811590 cites W2755429532 @default.
• W4306811590 cites W2780290167 @default.
• W4306811590 cites W2804815996 @default.
• W4306811590 cites W2811359070 @default.
• W4306811590 cites W2898776257 @default.
• W4306811590 cites W2900234070 @default.
• W4306811590 cites W2901648220 @default.
• W4306811590 cites W2903852362 @default.
• W4306811590 cites W2905349504 @default.
• W4306811590 cites W2921646015 @default.
• W4306811590 cites W2953089157 @default.
• W4306811590 cites W2983314124 @default.
• W4306811590 cites W2990564187 @default.
• W4306811590 cites W3011701994 @default.
• W4306811590 cites W3020606853 @default.
• W4306811590 cites W3052698549 @default.
• W4306811590 cites W3093233531 @default.
• W4306811590 cites W3168555285 @default.
• W4306811590 cites W3181421594 @default.
• W4306811590 cites W3186676469 @default.
• W4306811590 doi "https://doi.org/10.1016/j.ejpb.2022.10.007" @default.
• W4306811590 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36272655" @default.
• W4306811590 hasPublicationYear "2022" @default.
• W4306811590 type Work @default.
• W4306811590 citedByCount "1" @default.
• W4306811590 countsByYear W43068115902023 @default.
• W4306811590 crossrefType "journal-article" @default.
• W4306811590 hasAuthorship W4306811590A5000276308 @default.
• W4306811590 hasAuthorship W4306811590A5001634837 @default.
• W4306811590 hasAuthorship W4306811590A5002078560 @default.
• W4306811590 hasAuthorship W4306811590A5006775077 @default.
• W4306811590 hasAuthorship W4306811590A5009283691 @default.
• W4306811590 hasAuthorship W4306811590A5010962396 @default.
• W4306811590 hasAuthorship W4306811590A5020871813 @default.
• W4306811590 hasAuthorship W4306811590A5072870154 @default.
• W4306811590 hasAuthorship W4306811590A5075966059 @default.
• W4306811590 hasAuthorship W4306811590A5088212001 @default.
• W4306811590 hasConcept C104317684 @default.
• W4306811590 hasConcept C109825262 @default.
• W4306811590 hasConcept C147483822 @default.
• W4306811590 hasConcept C153911025 @default.
• W4306811590 hasConcept C159654299 @default.
• W4306811590 hasConcept C178790620 @default.
• W4306811590 hasConcept C179223381 @default.
• W4306811590 hasConcept C180577832 @default.
• W4306811590 hasConcept C185592680 @default.
• W4306811590 hasConcept C202751555 @default.
• W4306811590 hasConcept C2776403692 @default.

• next